DNA宏条形码技术在动植物法医鉴定中的应用进展

doi:10.12302/j.issn.1000-2006.202005015

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (1): 235-241.doi: 10.12302/j.issn.1000-2006.202005015

DNA宏条形码技术在动植物法医鉴定中的应用进展

李磊¹^,²(), 蒋敬², 陈云霞²^,^*()

1.南京林业大学,江苏南京 210037
2.南京森林警察学院,江苏南京 210023

收稿日期:2020-05-12 出版日期:2021-01-30 发布日期:2021-02-01
通讯作者: 陈云霞
基金资助:
中央高校基本科研业务费资助项目(LGZD201908);江苏省应用型本科院校建设与发展研究课题(2019yl05);江苏省高校优秀科技创新团队项目;江苏高校“青蓝工程”项目

Recent advances in the application of DNA metabarcoding technology in forensic identification of animals and plants

LI Lei¹^,²(), JIANG Jing², CHEN Yunxia²^,^*()

1. Nanjing Forestry University, Nanjing 210037, China
2. Nanjing Forest Police College, Nanjing 210023, China

Received:2020-05-12 Online:2021-01-30 Published:2021-02-01
Contact: CHEN Yunxia

摘要/Abstract

摘要：

DNA宏条形码技术是随着2代高通量测序技术的发展而出现的一种物种鉴别技术。该技术结合了DNA条形码技术和高通量测序技术的优点,可以对混合样本中的多物种来源进行同步鉴定。本研究介绍DNA宏条形码技术的含义以及目前的应用需求,分析DNA宏条形码技术在食品、药品监管、打击野生动植物犯罪、刑事案件侦查等司法实践中的应用现状,总结和讨论DNA宏条形码技术应用于动植物法医鉴定领域所面临的问题与挑战,对该技术的发展及应用前景进行展望。目前DNA宏条形码技术已被应用于生命科学的多个领域,但在动植物法医鉴定中的应用仍在起步阶段,虽然现在还面临着许多挑战,但随着测序技术与生物技术的发展,DNA宏条形码技术一定会在动植物法医鉴定领域实现常规化应用,为野生动植物相关犯罪活动的打击、食品药品安全性与合法性的监管提供技术支持。

关键词: DNA条形码, DNA宏条形码, 高通量测序, 动植物物种鉴定

Abstract:

DNA metabarcoding is a new technology for species identification that has emerged in recent years with the development of high-throughput sequencing techniques. This technology combines the advantages of DNA barcoding technology and high-throughput sequencing technology, which can simultaneously identify the source of multiple species in the mixed samples. This review introduces the definition of DNA metabarcoding technology and its current application requirements. Then, we review the application status of DNA metabarcoding technology in judicial practice, such as food and drug supervision, wildlife crime fighting and criminal investigations. Finally, we summarize the difficulties and challenges of metabarcoding in the field of forensic identification of animals and plants, and discuss the development and application prospects of this technology. At present, DNA metabarcoding technology has been applied in many fields of life sciences, but its use in the forensic identification of animals and plants is still in its early stage. Although it still faces many challenges, with the rapid development of sequencing technology and biotechnology, DNA metabarcoding technology will achieve routine applications in the field of forensic identification of animals and plants, providing technical support to combat wildlife-related criminals and supervise food and drug safety and legality.

Key words: DNA barcoding, DNA metabarcoding, high-throughput generation sequencing, species identification of animals and plants

中图分类号:

李磊,蒋敬,陈云霞. DNA宏条形码技术在动植物法医鉴定中的应用进展[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 235-241.

LI Lei, JIANG Jing, CHEN Yunxia. Recent advances in the application of DNA metabarcoding technology in forensic identification of animals and plants[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(1): 235-241.DOI: 10.12302/j.issn.1000-2006.202005015.

参考文献 37

[1]	SHOKRALLA S, SPALL J L, GIBSON J F, et al. Next-generation sequencing technologies for environmental DNA research[J]. Mol Ecol, 2012,21(8):1794-1805. DOI: 10.1111/j.1365-294x.2012.05538.x. doi: 10.1111/j.1365-294X.2012.05538.x pmid: 22486820
[2]	HEBERT P D N, CYWINSKA A, BALL S L, et al. Biological identifications through DNA barcodes[J]. Proceedings Biological sciences, 2003,270:313-321. DOI: 10.1098/rspb.2002.2218.
[3]	杨倩倩, 刘苏汶, 俞晓平. DNA条形码分析方法研究进展[J]. 应用生态学报, 2018,29(3):1006-1014.
	YANG Q Q, LIU S W, YU X P. Research progress on DNA barcoding analysis methods[J]. Chinese J of App Ecol, 2018,29(3):1006-1014. DOI: 10.13287/j.1001-9332.201803.032.
[4]	RATNASINGHAM S, HEBERT P D N. BOLD: the barcode of life data system[J]. Molecular Ecology Notes, 2007,7(3):355-364. DOI: 10.1111/j.1471-8286.2007.01678.x. doi: 10.1111/j.1471-8286.2007.01678.x pmid: 18784790
[5]	凌胜男, 吴亚君, 韩建勋, 等. DNA条形码技术在深加工动物制品源性成分鉴定中的应用研究进展[J]. 肉类研究, 2017,31(1):48-54.
	LING S N, WU Y J, HAN J X, et al. A review of recent advances in the application of dna barcoding in identification of animal species in highly processed animal-derived products[J]. Meat Research, 2017,31(1):48-54.DOI: 10.7506/rlyj1001-8123-201701009.
[6]	PALMER J D, HERBON L A. Plant mitochondrial DNA evolved rapidly in structure, but slowly in sequence[J]. J Mol Evol, 1988,28(1-2):87-97. DOI: 10.1007/bf02143500. doi: 10.1007/BF02143500 pmid: 3148746
[7]	王沙沙. 冬虫夏草线粒体基因特异性片段筛选与PCR鉴定研究[D]. 成都:成都中医药大学, 2019.
	WANG S S. The study of selecting mitochondrial gene specific fragments and PCR identification for Ophiocordyceps sinensis[D]. Chengdu:Chengdu University of Traditional Chinese Medicine, 2019. DOI: 10.26988/d.cnki.gcdzu.2019.000371.
[8]	辛天怡. 长白山区药用植物DNA条形码数据库构建及两种中成药生物组分监测研究[D]. 北京:北京协和医学院, 2019.
	XIN T Y. Construction of DNA barcode database of medicinal plants in Changbai Mountain and monitoring of biological components of two Chinese patent medicine[D]. Beijing: Peking Union Medical College, 2019.
[9]	Plant Working Group. A DNA barcode for land plants[J]. Proceedings of the National Academy of Sciences, 2009,106(31):12794-12797. DOI: 10.1073/pnas.0905845106.
[10]	张柠, 赵斐, 杨朔, 等. DNA条形码技术在法医学中的研究进展[J]. 昆明医科大学学报, 2018,39(10):130-133.
	ZHANG N, ZHAO F, YANG S, et al. Research progress of DNA barcoding technology in forensic medicine[J]. Journal of Kunming Medical University, 2018,39(10):130-133.
[11]	ANSORGE W J. Next-generation DNA sequencing techniques[J]. N Biotechnol, 2009,25:195-203. DOI: 10.1016/j.nbt.2008.12.009. doi: 10.1016/j.nbt.2008.12.009 pmid: 19429539
[12]	岳桂东, 高强, 罗龙海, 等. 高通量测序技术在动植物研究领域中的应用[J]. 中国科学:生命科学, 2012,42(2):107-124.
	YUE G D, GAO Q, LUO L H, et al. The application of high-throughput sequencing technology in plant and animal research[J]. SCIENTIA SINICA Vitae, 2012,42(2):107-124.DOI: 10.1360/052011-634.
[13]	张丁予, 章婷曦, 王国祥. 第二代测序技术的发展及应用[J]. 环境科学与技术, 2016,39(9):96-102.
	ZHANG D Y, ZHANG T X, WANG G X. Development and application of second-generation sequencing technology[J]. Environmental Science & Technology, 2016,39(9):96-102. DOI: 10.3969/j.issn.1003-6504.2016.09.017.
[14]	LOMAN N J, MISRA R V, DALLMAN T J, et al. Performance comparison of benchtop high-throughput sequencing platforms[J]. Nat Biotechnol, 2012,30(5):434-439. DOI: 10.1038/nbt.2198. doi: 10.1038/nbt.2198 pmid: 22522955
[15]	SALIPANTE S J, KAWASHIMA T, ROSENTHAL C, et al. Performance comparison of illumina and ion torrent next-generation sequencing platforms for 16S rRNA-based bacterial community profiling[J]. Appl Environ Microbiol, 2014,80(24):7583-7591. DOI: 10.1128/aem.02206-14. doi: 10.1128/AEM.02206-14 pmid: 25261520
[16]	MARDIS E R. The impact of next-generation sequencing technology on genetics[J]. Trends in Genetics, 2008,24(3):133-141. DOI: 10.1016/j.tig.2007.12.007. doi: 10.1016/j.tig.2007.12.007 pmid: 18262675
[17]	杜玲, 刘刚, 陆健, 等. 高通量测序技术的发展及其在生命科学中的应用[J]. 中国畜牧兽医, 2014(12):116-123.
	DU L, LIU G, LU J, et al. Development of high-throughput sequencing technology and its application in bioscience[J]. Chinese Animal Husbandry & Veterinary Medicine, 2014(12):116-123.DOI: CNKI:SUN:GWXK.0.2014-12-028.
[18]	PROSSER S W J, HEBERT P D N. Rapid identification of the botanical and entomological sources of honey using DNA metabarcoding[J]. Food Chemistry, 2017,214:183-191. DOI: 10.1016/j.foodchem.2016.07.077. doi: 10.1016/j.foodchem.2016.07.077 pmid: 27507464
[19]	邢冉冉, 吴亚君, 陈颖. 宏条形码技术在食品物种鉴定中的应用及展望[J]. 食品科学, 2018,39(13):280-288.
	XING R R, WU Y J, CHEN Y. DNA metabarcoding in food species identification: current applications and future prospects[J]. Food Science, 2018,39(13):280-288. DOI: 10.7506/spkx1002-6630-201813042.
[20]	STEFANIE D, MARION B, THOMAS W, et al. Development of a DNA metabarcoding method for the identification of fifteen mammalian and six poultry species in food[J]. Food Chemistry, 2018:S030881461831433X-. DOI: 10.1016/j.foodchem.2018.08.032. doi: 10.1016/j.foodchem.2021.129150 pmid: 33513529
[21]	蔡一村, 林颖峥, 刘群秀, 等. 高通量二代测序基因条形码技术在动物源性制品物种鉴定中的应用[J]. 现代食品科技, 2020,36(3):252-259.
	CAI Y C, LIN Y Z, LIU Q X, et al. Application of high-throughput sequencing and gene barcode technology in species identification of animal-derived products[J]. Modern Food Science and Technology, 2020,36(3):252-259. DOI: 10.13982/j.mfst.1673-9078.2020.3.033.
[22]	张恩迪, 李冰. 中药资源与濒危野生动植物保护[M]. 上海: 上海中医药大学出版社, 2004.
	ZHANG E D, LI B. Traditional Chinese medicine resources and protection of endangered wildlife[M]. Shanghai: Shanghai University of Traditional Chinese Medicine Press, 2004.
[23]	MARTIJN S, ALFRED J A, BARBARA G, et al. Advances in DNA metabarcoding for food and wildlife forensic species identification[J]. Anal Bioanal Chem, 2016,408:4615-4630. DOI: 10.1007/s00216-016-9595-8 doi: 10.1007/s00216-016-9595-8 pmid: 27178552
[24]	COGHLAN M L, HAILE J, HOUSTON J, et al. Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns[J]. PLoS Genetics, 2012,8(4):e1002657.DOI: 10.1371/journal.pgen.1002657. doi: 10.1371/journal.pgen.1002657 pmid: 22511890
[25]	CHENG X W, SU X Q, CHEN X H, et al. Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan[J]. Sci Rep, 2014(4):e5147. DOI: 10.1038/srep05147.
[26]	RACLARIU A C, MOCAN A, POPA M O, et al. Veronica officinalis product authentication using DNA metabarcoding and HPLC-MS reveals widespread adulteration with Veronica chamaedrys[J]. Front Pharmacol, 2017,8:378. DOI: 10.3389/fphar.2017.00378. doi: 10.3389/fphar.2017.00378 pmid: 28674497
[27]	石林春, 刘金欣, 宋经元, 等. 基于DNA metabarcoding 技术的如意金黄散处方成分鉴定研究[J]. 中国科学: 生命科学, 2018,48(4):490-497.
	SHI L C, LIU J X, SONG J Y, et al. DNA metabarcoding identification of prescription ingredients in traditional medicine Ruyi Jinhuang San[J]. Sci Sin Vitae, 2018,48(4):490-497.DOI: 10.1360/N052017-00204.
[28]	刘萌妍, 刘艳磊, 吴平, 等. 利用人体携带植物基因信息推断居住地[J]. 法医学杂志, 2019,35(6):710-715.
	LIU M Y, LIU Y L, WU P, et al. Determination of place of residence using the gene information of plants carried by the human body[J]. Journal of Forensic Medicine, 2019,35(6):710-715.DOI: 10.12116/j.issn.1004-5619.2019.06.012.
[29]	BERNARDO G D, GAUDIO S D, GALDERISI U, et al. Comparative evaluation of different DNA extraction procedures from food samples[J]. Biotechnol Progr, 2007,23(2):297-301. DOI: 10.1021/bp060182m. doi: 10.1021/bp060182m
[30]	FERNANDES T J, OLIVEIRA M B P, MAFRA I. Tracing transgenic maize as affected by breadmaking process and raw material for the production of a traditional maize bread, broa[J]. Food Chem, 2013,138(1):687-692. DOI: 10.1016/j.foodchem.2012.10.068. doi: 10.1016/j.foodchem.2012.10.068
[31]	GRYSON N. Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review[J]. Anal Bioanal Chem, 2010,396(6):2003-2022. DOI: 10.1007/s00216-009-3343-2. doi: 10.1007/s00216-009-3343-2 pmid: 20012944
[32]	马兰. DNA微型条形码和复合条形码研究进展[J]. 农业灾害研究, 2013,3(6):58-60,63.
	MA L. Research progress in DNA minibarcoding and metabarcoding[J]. Journal of Agricultural Catastrophology, 2013,3(6):58-60,63. DOI: 10.19383/j.cnki.nyzhyj.2013.06.021.
[33]	高梓童. 中成药的DNA条形码鉴定: 从mini-barcode到meta-barcode[D]. 北京:北京协和医学院, 2019.
	GAO Z T. DNA barcode identification of Chinese medicine: from mini-barcode to meta-barcode[D]. Beijing: Peking Union Medical College, 2019.
[34]	王荣亮, 段润平, 包丹, 等. 从物种识别到生物多样性评估: DNA条形码与DNA metabarcoding技术[J]. 生物学通报, 2016,51(4):10-13.
	WANG R L, DUAN R P, BAO D, et al. From species identification to biodiversity assessment: DNA barcode and DNA metabarcoding technology[J]. Bulletin of Biology, 2016,51(4):10-13. DOI: CNKI:SUN:SWXT.0.2016-04-003.
[35]	COISSAC E, RIAZ T, PUILLANDRE N. Bioinformatic challenges for DNA metabarcoding of plants and animals[J]. Molecular Ecology, 2012,21(8):1834-1847. DOI: 10.1111/j.1365-294x.2012.05550.x. doi: 10.1111/j.1365-294X.2012.05550.x pmid: 22486822
[36]	田李, 张颖, 赵云峰. 新一代测序技术的发展和应用[J]. 生物技术通报, 2015,31(11):1-8.
	TIAN L, ZHANG Y, ZHAO Y F. The next generation sequencing technology and its applications[J]. Biotechnology Bulletin, 2015,31(11):1-8. DOI: 10.13560/j.cnki.biotech.bull.1985.2015.11.003.
[37]	ARULANDHU A J, STAATS M, HAGELAAR R, et al. Development and validation of a multi-locus DNA metabarcoding method to identify endangered species in complex samples[J]. Gigascience, 2017,6:1-18. DOI: 10.1093/gigascience/gix080. doi: 10.1093/gigascience/gix090 pmid: 29048539

DNA宏条形码技术在动植物法医鉴定中的应用进展

Recent advances in the application of DNA metabarcoding technology in forensic identification of animals and plants

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