当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化

doi:10.12302/j.issn.1000-2006.202204017

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2): 45-50.doi: 10.12302/j.issn.1000-2006.202204017

• 专题报道Ⅱ: 乡村振兴视域下特色林果培育专题（执行主编李维林方升佐） • 上一篇下一篇

当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化

赵晓龙¹(), 沈家怡¹, 刘涛², 吴家胜¹, 胡渊渊¹^,^*()

1.亚热带森林培育国家重点实验室,浙江农林大学林业与生物技术学院,浙江杭州 311300
2.杭州市原种场,浙江杭州 311115

收稿日期:2022-03-04 修回日期:2022-04-18 出版日期:2024-03-30 发布日期:2024-04-08
通讯作者: *胡渊渊(hyy_1985@zafu.edu.cn),教授。
作者简介:赵晓龙(zhaoxiaolong199860@qq.com)。
基金资助:
国家自然科学基金区域创新发展联合基金(U20A2049);国家自然科学面上基金项目(32271922)

Seasonal variations in photosynthetic efficiency and antioxidant characteristics of the current and one year-old leaves in Torreya grandis‘Merrillii’

ZHAO Xiaolong¹(), SHEN Jiayi¹, LIU Tao², WU Jiasheng¹, HU Yuanyuan¹^,^*()

1. State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A & F University, Hangzhou 311300, China
2. Hangzhou Raw Seed Growing Farm, Hangzhou 311115, China

Received:2022-03-04 Revised:2022-04-18 Online:2024-03-30 Published:2024-04-08

摘要/Abstract

摘要：

【目的】通过比较分析香榧(Torreya grandis ‘Merrillii’)主要功能叶的叶绿素荧光参数、抗氧化酶活性及丙二醛(MDA)含量等参数的季节性变化,初步探讨香榧叶片光合特性对高温、低温的温度变化的适应机制。【方法】以人工林中16年生香榧的当年生叶片和越年生叶片为研究对象,测定5、6、8、11月和次年1月中旬香榧叶片的叶绿素荧光参数、比叶质量(SLW)、抗氧化酶活性及可溶性蛋白含量的变化。【结果】 ①与5月中旬相比,8月中旬香榧当年生和越年生叶片光系统Ⅱ(PSⅡ)的实际最大光化学量子效率(Φ_PSⅡ)均明显上调,而PSⅡ处调节的能量耗散量子效率(Φ_NPQ)均明显下调,其PSⅡ处非调节性能量热耗散量子效率(Φ_NO)未发生明显变化;香榧当年生叶片的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均明显增强,过氧化物酶(POD)活性则明显减弱,丙二醛(MDA)含量则无明显变化。②与11月中旬相比, 次年1月的平均温度约降低了近10 ℃,香榧当年生和越年生叶片的Φ_PSⅡ和Φ_NPQ均显著降低,而其Φ_NO呈明显增加趋势,且MDA含量均呈显著增加趋势。【结论】与5月中旬相比,8月中旬温度较高,香榧叶片可以吸收较多光能,并用于电子传递,以热的形式耗散能量较少,其光合机构未发生光抑制,其较强的抗氧化酶体系能协调地助其适应高温高光环境,因此其当年生叶片和越年生叶片的Φ_PSⅡ明显增强;与11月中旬相比,次年1月中旬香榧当年生叶片和越年生叶片在越冬期间受到了冷害,叶片光合机构受到光损伤。综上所述,香榧叶片对环境表现为耐热不耐冷,这可能是其分布北扩的限制因素。

关键词: 香榧, 叶龄, 抗氧化酶, 光合特性, 季节性变化

Abstract:

【Objective】 Determination of seasonal changes in chlorophyll fluorescence parameters, antioxidant enzymes, malondialdehyde (MDA) and other parameters in current and 1 year-old leaves of Torreya grandis ‘Merrillii’ was to study the adaptive mechanism of the photosynthetic characteristics of T. grandis ‘Merrillii’ leaves to temperature changes during season. 【Method】 The chlorophyll fluorescence parameters, specific leaf weight (SLW), antioxidant enzyme activity and soluble protein changes of T. grandis ‘Merrillii’ leaves were measured in May, June, August, November and January of the second year. 【Result】 (1) Compared with mid-May, Φ_PSⅡ of T. grandis ‘Merrillii’ leaves in mid-August was significantly up-regulated, while Φ_NPQ was significantly down-regulated, and Φ_NOwith little change. SOD and CAT activities in T. grandis ‘Merrilli’ current leaves were significantly increased, POD activity was significantly decreased, and MDA with little change.(2) Compared with mid-November, the average temperature in January of the second year decreased by nearly 10 ℃. Φ_PSⅡ and Φ_NPQ of T. grandis ‘Merrillii’ current leaves and one-year old leaves decreased significantly, while their Φ_NOand MDA content increased significantly. 【Conclusion】 Compared with mid-May, T. grandis ‘Merrillii’ current leaves and one-year old leaves markedly improved Φ_PSⅡ in mid-August is due to the high temperature and its leaves to absorb more light, and used for electron transfer, less dissipation of energy in the form of heat, the photosynthetic photoinhibition not occurring, its strong antioxidant enzyme system can coordinate to help them adapt to the high temperature specular environment. Compared with mid-November, T. grandis ‘Merrillii’ current leaves and one-year old leaves suffered cold damage during overwintering in mid-January of the second year, and the photosynthetic organs of leaves suffered light damage. Above all, T. grandis ‘Merrillii’ leaves are resistant to heat weather but not to cold weather, which may be the limiting factor of northern expansion.

Key words: Torreya grandis ‘Merrillii’, different leaf age, antioxidant enzymes, photosynthetic characteristics, seasonal change

中图分类号:

S791.43

赵晓龙,沈家怡,刘涛,等. 当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 45-50.

ZHAO Xiaolong, SHEN Jiayi, LIU Tao, WU Jiasheng, HU Yuanyuan. Seasonal variations in photosynthetic efficiency and antioxidant characteristics of the current and one year-old leaves in Torreya grandis‘Merrillii’[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(2): 45-50.DOI: 10.12302/j.issn.1000-2006.202204017.

图/表 4

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月份 month	平均最高气温 mean max air temperature	平均最低气温 mean min air temperature
5	28.06±3.63 b	18.26±2.00 b
6	27.83±2.41 b	21.40±1.55 b
8	35.06±2.10 a	26.71±1.04 a
11	17.80±2.33 c	11.37±2.74 c
1	8.09±4.25 d	1.81±3.64 d

当年和越年生香榧叶片的光合效率及抗氧化特性的季节性变化

Seasonal variations in photosynthetic efficiency and antioxidant characteristics of the current and one year-old leaves in Torreya grandis‘Merrillii’

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