通过野外定位观测,对鹅毛竹与异叶苦竹的开花物候、开花动态及繁育系统、花粉-胚珠比(P/O)、杂交指数(OCI)进行了观察和检测。在显微镜下观测柱头及花粉-胚珠比,用联苯胺-过氧化氢法测定柱头可授性,用套袋法检测繁育系统。结果表明:鹅毛竹在上年的10月进行花芽的分化,盛花期为次年的3月,花芽的形成到开花跨越2个年度; 异叶苦竹当年3月形成花芽,其盛花期为4月。在各种套袋试验中,鹅毛竹均不结实; 而异叶苦竹收获有数量不等的种子。繁育系统检测表明,异叶苦竹和鹅毛竹无融合生殖现象,异叶苦竹的繁育系统以异交为主,部分自交亲和,需要传粉者。
Abstract
By means of orientational observation in the field, flowering dynamic, breeding system, P/O and OCI of the two bamboo species, Shibataea chinensis and Arundinaria simonii f. heterophylla, were determined. The stigma receptivity was estimated by benzidine-H2O2, the pollen-ovule ratio, morphology of stigma were examined with microscopy, and bagging was used to examine the breeding system.The main results were as follows:the flower buds differentiated in October last year and bloomed in March this year for Shibataea chinensis. While the flower buds differentiated in March and bloomed in April for Arundinaria simonii f. heterophylla. Artificial pollination can improve the seed setting. The seeds could be harvested more or less every year. However, Shibataea chinensis never bore any seeds. The breeding system of Arundinaria simonii f. heterophylla is mainly outcrossing, but partly may be self-compatible.
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参考文献
[1] Li Y F,Zhou G M, Jiang P K,et al. Carbon accumulation and carbon forms in tissues during the growth of young bamboo(Phyllostachy pubescens)[J].The Botanical Review,2011,77(3):278-286.
[2] Zhang F,Wan X Q,Zhang H Q,et al.The effect of cold stress on endogenous hormones and CBF1 homolog in four contrasting bamboo species[J]. Journal of Forest Research, 2012,17(1):72-78.
[3] 高志民,杨学文,彭镇华,等.绿竹BoSUT2基因的克隆、分析与亚细胞定位研究[J].林业科学,2010,46(2):45-49. Gao Z M,Yang X W,Peng Z H,et al. Molecular characterization and subcellular localization of BoSUT2 from Bambusa oldhamii[J]. Scientia Silvae Sinicae, 2010,46(2):45-49.
[4] Huang S Q, Yang C F, Lu B, Takahashi Y, et al. Honeybee-assisted wind pollination in bamboo Phyllostachys nidularia(Bambusoideae:Poaceae)[J] Botanical Journal of the Linnean Society, 2002, 138:1-7.
[5] Ramanayake S M S D, Weerawardene T E. Flowering in a bamboo, Melocanna baccifera(Bambusoideae:Poaceae)[J]. Botanical Journal of the Linnean Society, 2003, 143:287-291.
[6] 林树燕.鹅毛竹和异叶苦竹的生殖生物学研究[D].南京:南京林业大学,2009 Lin S Y. Studies on the reproductive biology of Shibataea chinensis and Arundinaria Simonii f. Albostriatus[D].Nanjing:Nanjing Forestry University,2009.
[7] Dafni A. Pollination Ecology [M].New York:Oxford Univ Press,1992.
[8] Cruden R W. Pollen-ovule rations:a conservative indicator of breeding systems in flowering plants[J]. Evolution,1977,31:32-36.
[9] 张志良.植物生理学实验指导[M]. 3版.北京:高等教育出版社,2003.
[10] 张文燕,马乃训.竹类植物花粉的生活力和自然受粉[J].林业科学研究, 1990, 3(3):250-55. Zhang W Y, Ma N X. The research on the pollen viability and natural pollination of bamboo plants[J]. Research of the Forestry Science, 1990, 3(3):250-55.
[11] 李昆,尹伟伦,罗长维.小桐子繁育系统与传粉生态学研究[J].林业科学研究,2007,20(6):775-781. Li K, Yin W L, Luo C W. Breeding system and pollination ecology in Jatropha curcas[J]. Forest Research, 2007,20(6):775-781.
[12] 朱念德.植物学(形态解剖部分)[M].广州:中山大学出版社, 2000.
[13] McClure F A.New genera and species of Bambusaceae from eastern[J].Asia Lingnan Science Bulletin,1940,9:66-67.
[14] 耿伯介.世界竹亚科各属的考订[J].竹子研究汇刊,1982,1(1):1-19. Geng B J. The research on each genus of the Bambusoideae [J]. Journal of Bamboo Research, 1982,1(1):1-19.
[15] 耿以礼.中国主要植物图说——禾本科[M].北京:科学出版社,1959.
[16] 方伟.竹子分类学[M].北京:中国林业出版社,1995.
[17] 袁晓亮, 雷竹花期生物学特性与花发育解剖学研究[D].临安:浙江林学院,2007. Yuan X L. Studies on florescence biological characteristics and flower developmental anatomy of Phyllostachys praecox[D].Lin’an:Zhejiang Forestry College,2007.
[18] 林树燕,丁雨龙.鹅毛竹大小孢子及雌雄配子体发育[J].西北植物学报,2012,32(5):907-914. Lin S Y, Ding Y L. Development of the male and female gametophytes in Shibataea chinensis(Bambusoideae)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2012,32(5):907-914.
[19] 林树燕,丁雨龙,张昊.5种竹子花粉萌发率及开花特性[J].林业科学,2008,44(10):159-163. Lin S Y, Ding Y L, Zhang H. Pollen germination percentage and the floral character of five bamboo species[J]. Scientia Silvae Sinicae, 2008,44(10):159-163.
[20] 林树燕,骆仁祥,丁雨龙,等. 月月竹的花粉萌发力及贮藏力研究[J].林业科技开发,2008,22(5):22-25. Lin S Y, Luo R X, Ding Y L, et al.Studies on the pollen vitality and storage capacity in Chimonobambusa szechuanensis[J]. China Forestry Science and Technology, 2008,22(5):22-25.
[21] 刘林德,祝宁,申家恒,等. 刺五加、短梗五加的开花动态及繁育系统的比较研究[J].生态学报, 2002,22(07):1041-1048. Liu L D, Zhu N, Shen J H, et al. Comparative studies on floral dynamics and breeding system between Eleutherococcus senticosus and E.sessiliflorus[J].Acta Ecologica Sinica, 2002,22(07):1041-1048.
[22] 肖宜安,何平,李晓红.濒危植物长柄双花木的花部综合特征与繁育系统[J].植物生态学报, 2004, 28(3):333-340 Xiao Y A, He P, Li X H. Floral syndrome and breeding system of the endangered plant Disanthus cercidifolius Maxim. var. longipes[J]. Acta Phytoecologica Sinica,2004, 28(3):333-340.
[23] 张仁波,窦全丽,何平,等. 濒危植物缙云卫矛繁育系统研究[J].广西植物,2006,26(3):308-312 Zhang R B, Dou Q L, He P, et al. Study on the breeding system of the endangered plant Euonymus chloranthoides Yang[J]. Guihaia, 2006,26(3):308-312.
基金
收稿日期:2011-11-20 修回日期:2012-12-20
基金项目:国家自然科学基金项目(31000294)
第一作者:林树燕,副教授,博士。*通信作者:丁雨龙,教授,博士。E-mail: ylding@vip.163.com。
引文格式:林树燕,丁雨龙. 鹅毛竹与异叶苦竹的繁育系统研究[J]. 南京林业大学学报:自然科学版,2013,37(3):1-5.
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