拟南芥、水稻和毛果杨中CesA基因的进化和表达分析

doi:10.3969/j.issn.1000-2006.2013.03.003

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (03): 11-16.doi: 10.3969/j.issn.1000-2006.2013.03.003

拟南芥、水稻和毛果杨中CesA基因的进化和表达分析

朱煜,谭梦月,邹爱兰,张文举,戚金亮,杨永华^*

南京大学生命科学学院,医药生物技术国家重点实验室,南京大学-南京林业大学植物分子生物学联合研究所, 江苏南京 210093

出版日期:2013-06-18 发布日期:2013-06-18
基金资助:
收稿日期:2012-02-09 修回日期:2013-03-08
基金项目:教育部创新团队项目(IRT1020)
第一作者:朱煜, 硕士生; 谭梦月,硕士生。*通信作者:杨永华,教授。E-mail:yangyh@nju.edu.cn。
引文格式:朱煜,谭梦月,邹爱兰,等. 拟南芥、水稻和毛果杨中CesA基因的进化和表达分析[J]. 南京林业大学学报:自然科学版,2013,37(3):11-16.

Phylogenetic and expression analysis of CesA genes in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa

ZHU Yu, TAN Mengyue, ZOU Ailan, ZHANG Wenju, QI Jinliang, YANG Yonghua^*

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, NJU-NJFU Plant Molecular Biology Institute, Nanjing University, Nanjing 210093,China

Online:2013-06-18 Published:2013-06-18

摘要/Abstract

摘要： 南京大学生命科学学院,医药生物技术国家重点实验室,南京大学-南京林业大学植物分子生物学联合研究所, 江苏南京 210093

Abstract: Cellulose is the main component of plant cell walls, and the cellulose synthase encoded by CesA gene is the most important enzyme involved in cellulose biosynthesis. In this study, genome sequence of three model plant species(Arabidopsis thaliana, Oryza sativa, Populus trichocarpa)had been utilized to investigate the phylogenetic relationship of the CesA gene family. The results showed that CesA genes had already existed before species differentiation and that gene expansion occurred after species differentiation. There was a variation in the degree of gene expansion among different species and the entire gene family showed negative selection pressure. To further demonstrate the expression regulation patterns of CesA genes, the promoters of OsCesAs were analyzed and the results suggest that OsCesA genes might be regulated by various stresses and hormones. RT-PCR results indicated that some OsCesAs were down-regulated by abscisic acid.

中图分类号:

Q756

朱煜,谭梦月,邹爱兰,等. 拟南芥、水稻和毛果杨中CesA基因的进化和表达分析[J]. 南京林业大学学报（自然科学版）, 2013, 37(03): 11-16.

ZHU Yu, TAN Mengyue, ZOU Ailan, ZHANG Wenju, QI Jinliang, YANG Yonghua. Phylogenetic and expression analysis of CesA genes in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa[J].Journal of Nanjing Forestry University (Natural Science Edition), 2013, 37(03): 11-16.DOI: 10.3969/j.issn.1000-2006.2013.03.003.

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拟南芥、水稻和毛果杨中CesA基因的进化和表达分析

Phylogenetic and expression analysis of CesA genes in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa

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