低温胁迫下冬青叶片细胞内Ca2+水平及可溶性糖含量的变化

赵明明; 周余华; 彭方仁; 郝明灼; 梁有旺; 任莺; 华宏

doi:10.3969/j.issn.1000-2006.2013.05.001

低温胁迫下冬青叶片细胞内Ca²⁺水平及可溶性糖含量的变化

赵明明，周余华,彭方仁*，郝明灼,梁有旺,任莺，华宏

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 1-5.

PDF(2120239 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2120239 KB)

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 1-5. DOI: 10.3969/j.issn.1000-2006.2013.05.001

研究论文

低温胁迫下冬青叶片细胞内Ca²⁺水平及可溶性糖含量的变化

赵明明¹，周余华²,彭方仁¹*，郝明灼¹,梁有旺¹, 任莺³，华宏³

作者信息 +

Changes of Ca2+ level and soluble sugar content in cells of Ilex L. leaflets under low temperature stress

ZHAO Mingming¹, ZHOU Yuhua², PENG Fangren¹*, HAO Mingzhuo¹, LIANG Youwang¹, REN Ying³, HUA Hong³

Author information +

文章历史 +

摘要

以杂种冬青（Ilex×‘Nellie R.Steven’）、大别山冬青（I. dabieshannensis）、蓝少女冬青（I. ×mersevae ‘Blue Gir’）、蓝少年冬青（I.× mersevae ‘Blue Boy’）2年生扦插苗为材料，研究了低温处理后冬青叶片细胞内Ca2+ 水平及可溶性糖含量的变化。结果表明:冬青叶片细胞内Ca2+沉淀随温度的降低而转移并增加，细胞膜系统、叶绿体、细胞核等结构都受到不同程度的破坏，可溶性糖含量均随着处理温度的降低而增加。4种冬青的抗寒性由高至低依次为：杂种冬青>蓝少女冬青>蓝少年冬青>大别山冬青。

Abstract

In this paper, two years old seedlings of four types of Ilex×‘Nellie R.Steven’, I.dabieshannensis, I.× mersevae ‘Blue Gir’, I.×mersevae ‘Blue Boy’ were placed in different low temperature condition, the changes of Ca2+ level in the cells of Ilex L. leaf and changes of soluble sugar content under chilling stress were studied. The results showed that:as the temperature decreased, Ca2+ gradually transfered and deposits added in the cells of Ilex L. Leaf for all the tested samples, their membrane systems, organelle structure stabilities and nucleus structures were damaged at different degree and soluble sugar content showed an upward trend. The results revealed that cold resistance of four culttings from strong to weak were I.×‘Nellie R.Steven’>I. mersevae ‘Blue Gir’>I. mersevae ‘Blue Boy’>I. dabieshanensis.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

赵明明，周余华,彭方仁*，郝明灼,梁有旺,任莺，华宏. 低温胁迫下冬青叶片细胞内Ca²⁺水平及可溶性糖含量的变化[J]. 南京林业大学学报（自然科学版）. 2013, 37(05): 1-5 https://doi.org/10.3969/j.issn.1000-2006.2013.05.001

ZHAO Mingming, ZHOU Yuhua, PENG Fangren*, HAO Mingzhuo, LIANG Youwang, REN Ying, HUA Hong. Changes of Ca2+ level and soluble sugar content in cells of Ilex L. leaflets under low temperature stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(05): 1-5 https://doi.org/10.3969/j.issn.1000-2006.2013.05.001

中图分类号： S718

参考文献

［1］欧静. 贵州省冬青科野生观赏树木资源的园林利用[J]. 中国野生植物资源, 2002, 21(4):41-42. Ou J. The utilization of the resources of wild ornamental woody plants of Aquifoliaceae in Guizhou province[J]. Chinese Wild Plant Resources, 2002, 21(4):41-42.
［2］Taylor D W. Paleobiogeographic relationships of angiosperms from the Cretaceous and early Tertiary of the North American area[J]. The Botanical Review, 1990, 56(4):279-417.
［3］Pott R. Die nacheiszeitliche Ausbreitung und heutige pflanzensoziologische stellung von Ilex aquifolium L[J]. Tuexenia, 1990, 10:497-512.
［4］魏朝阳. 冬青种子休眠机理及解除方法研究[D]. 南京: 南京林业大学, 2007. Wei C Y.Studies on the mechanisms and breaking methods of Ilex purpurea seed dormancy[D].Nanjing:Nanjing Forestry University,2007.
［5］陆小清, 郑贵鸣, 李云龙, 等. 大别山冬青引种栽培技术[J]. 江苏林业科技, 2007, 34(5):32-33. Lu X Q, Zheng G M, Li Y L, et al. The introduction and cultivation technology of Ilex dabieshanensis[J]. Jiangsu Forestry Science and Technology, 2007, 34(5):32-33.
［6］Bush D S. Calcium regulation in plant cells and its role in signaling[J]. Annual Review of Plant Biology, 1995, 46(1):95-122.
［7］王红, 简令成, 张举仁. 低温胁迫下水稻幼叶细胞内Ca2+水平的变化[J]. 植物学报, 1994, 36(8):587-591. Wang H,Jian L C,Zhang J R. Changes of the Ca2+ in cells of rice seedlings under low temperature stress[J]. Journal of Integrative Plant Biology, 1994, 36(8):587-591.
［8］Minorsky P V. Temperature sensing by plants:a review and hypothesis[J]. Plant Cell Environ, 1989, 12(2):119- 135.
［9］Knight M R, Campbell A K, Smith S M, et al. Transgenic plant aequorin reports the effects of touch and cold shock and elicitors on cytoplasmic calcium[J]. Nature, 1991, 352:524-526.
［10]Olien C R. Preliminary classification of polysaccharide freezing inhibitors[J].Corp Science, 1967, 7(2):156-157.
［11]赵明明. 几种冬青属植物抗寒性比较研究[D]. 南京:南京林业大学, 2012. Zhao M M. Comparative study on cold resistance of several Ilex trees[J]. Nanjing:Nanjing Forestry University,2012.
［12]朱政, 蒋家月, 江昌俊. 低温胁迫对茶树叶片SOD、可溶性蛋白和可溶性糖含量的影响[J]. 安徽农业大学学报, 2011, 38 (1):24-26. Zhu Z,Jiang J Y,Jiang J C.Effects of low temperature stress on SOD activity, soluble protein content and soluble sugar content in Camellia sinensis leaves[J]. Journal of Anhui Agricultural University, 2011, 38(1):24-26.
［13]王荣富. 植物抗寒指标的种类及其应用[J]. 植物生理学通讯, 1987 (3):49-55. Wang R F.T he kinds of plant hardiness criteria and their application[J]. Plant Physiology Communication, 1987 (3):49-55.
［14]袁淑珍, 栗淑媛, 乔辰. 低温胁迫对螺旋藻体内可溶性糖含量的影响[J]. 中国农学通报, 2008, 24(5):113-116. Yuan S Z, Li S Y, Qiao C. The effect of low temperature stress on content of soluble sugar in Spirulina(Arthrospira) [J]. Chinese Agricultural Science Bulletin, 2008,24(5):113-116.
［15]Hepler P K. Calcium:a central regulator of plant growth and development[J]. Plant Cell, 2005, 17(8):2142- 2155.
［16]张展鹏. 植物Ca2+信号的研究进展[J]. 贵州农业科学, 2008, 36(3):26-28. Zhang Z P. Research progress on Ca2+ signal in plants[J]. Guizhou Agricultural Sciences, 2008, 36(3):26-28.
［17]王颖. 盐胁迫和干旱胁迫下胡杨和意大利杨细胞内Ca2+和Ca2+-ATPase水平变化[D]. 北京:北京林业大学, 2010. Wang Y. Changes of Ca2 +and Ca2+-ATPase activities in cells of Populus euphratica and P.euramericana under salt and drought stress[D].Beijing:Beijing Forestry University, 2010.
［18]姜勇, 罗深秋. 细胞信号转导的分子基础与功能调控[M]. 北京:科学出版社, 2005.
［19]许东新, 庄炳莉, 薛建辉, 等. 南京地区5个常绿阔叶树种的抗寒性评价[J]. 南京林业大学学报:自然科学版, 2010, 34(3):72- 76. Xu D X, Zhuang B L, Xue J H, et al.Evaluation on cold resistance of five evergreen broadleaved ornamental tree species in the area of Nanjing[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2010, 34(3):72-76.
［20]李小安. 低温胁迫对青藏扁蓿豆与和田苜蓿种子游离脯氨酸和可溶性糖含量的影响[J]. 青海大学学报:自然科学版, 2011, 29(4):10-13. Li X A. Effects of chilling stress on proline and soluble sugar content of Melilotus ruthenicus and Hetian alfalfa [J]. Journal of Qinghai University:Nature Science, 2011, 29(4):10-13.
［21]简令成, 王红. 钙(Ca2+)在植物抗寒中的作用[J]. 细胞生物学杂志, 2002, 24(3):166-171. Jian L C, Wang H. Effects of Ca2+ in plant cold resistance[J]. Chinese Journal of Cell Biology, 2002, 24(3):166-171.

基金

收稿日期：2012-06-16修回日期：2013-04-07 基金项目：江苏省林业三项工程项目（lysx[2010]20）第一作者：赵明明,硕士生。 *通信作者：彭方仁，教授。 Email： frpeng@njfu.edu.cn。引文格式：赵明明，周余华,彭方仁，等. 低温胁迫下冬青叶片细胞内Ca2+水平及可溶性糖含量的变化[J]. 南京林业大学学报：自然科学版，2013，37（5）：1-5.