枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定

doi:10.3969/j.issn.1000-2006.2014.01.033

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (01): 183-186.doi: 10.3969/j.issn.1000-2006.2014.01.033

枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定

程晓甜^1,2,阿地力·沙塔尔^1,2*,张伟³,李新泉^1,2

1.新疆农业大学林学与园艺学院,新疆乌鲁木齐 830052;
2.新疆农业大学,新疆教育厅干旱区林业生态与产业技术重点实验室,新疆乌鲁木齐 830052;
3.新疆出入境检验检疫局技术中心,新疆乌鲁木齐 830083

出版日期:2014-01-15 发布日期:2014-01-15
基金资助:
收稿日期:2012-12-31 修回日期:2013-10-09
基金项目:新疆维吾尔自治区科技计划项目(201130102-3); 新疆维吾尔自治区重点学科森林培育科研项目
第一作者:程晓甜,硕士生。*通信作者:阿地力·沙塔尔,教授。E-mail:adl1968@163.com。
引文格式:程晓甜,阿地力·沙塔尔,张伟,等. 枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定[J]. 南京林业大学学报:自然科学版,2014,38(1):183-185.

Rapid identification of Carpomya vesuviana Costa and five relative species of fruit flies by PCR-RFLP

CHENG Xiaotian^1,2,ADILI Sattar^1,2*,ZHANG Wei³,LI Xinquan^1,2

1.College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
2.Key Laboratory of Forestry Ecology and Industry Technology in Arid Region, Education Department of Xinjiang, Xinjiang Agricultural University, Urumqi 830052, China;
3.Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi 830083, China

Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要： 枣实蝇(Carpomya vesuviana Costa)是一种新入侵检疫性有害生物。应用PCR-RFLP技术,快速区分鉴定了枣实蝇与我国口岸截获频率较高的5种检疫性实蝇。研究表明:设计出的2对引物对6种供试实蝇线粒体DNA(mtDNA)进行PCR扩增,其扩增片断大小分别为350 bp和450 bp。PCR扩增产物用2种限制性内切酶Dra I和MseI进行酶切,根据不同的酶切位点准确区分了6种供试的实蝇。此方法可用于口岸截获实蝇的快速检疫鉴定。

Abstract: Carpomya vesuviana Costa has been a new quarantine pest in China. In this paper, the method of rapid identification of C. vesuviana Costa and 5 relative species of fruit flies was studied based on PCR-RFLP of the mitochondrial DNA(mtDNA). The results showed that DNAs, which were estimated about 350 bp and 450 bp respectively, were obtained by means of PCR with two pairs of primers designed from mtDNA of target pests.The PCR-products could be registered by restriction enzymes(Dra I and Mse I). All the 6 species could be discriminated according to the banding patterns of the restriction enzymes. It is suggested that the method is reliable for quick discrimination of fruit flies.

中图分类号:

Q965

程晓甜,阿地力·沙塔尔,张伟,等. 枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定[J]. 南京林业大学学报（自然科学版）, 2014, 38(01): 183-186.

CHENG Xiaotian,ADILI Sattar,ZHANG Wei,LI Xinquan. Rapid identification of Carpomya vesuviana Costa and five relative species of fruit flies by PCR-RFLP[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(01): 183-186.DOI: 10.3969/j.issn.1000-2006.2014.01.033.

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枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定

Rapid identification of Carpomya vesuviana Costa and five relative species of fruit flies by PCR-RFLP

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