研究不同贮藏方法对桂花花粉活力的影响,探索桂花花粉保存的适宜条件。以4个品种群中的9种桂花品种为试验材料,对其进行常温、低温、低温冷冻和超低温冷冻贮藏,并对不同贮藏时间内的花粉活力进行检测。结果表明:桂花花粉在10%的蔗糖、0.01%硼酸和1%的琼脂糖组成的固体培养基上,25 ℃恒温培养24 h后花粉基本充分萌发,可用于萌发率的统计; 光照和黑暗对花粉萌发无显著影响。不同贮藏条件下花粉保存效果差异显著,室温条件(15～20 ℃)下,大多数品种花粉寿命为15～20 d; 低温贮藏(4 ℃)花粉活力可延长至50 d,低温冷冻(-20 ℃)和超低温冷冻(-70 ℃)贮藏100 d后大多数桂花品种的花粉萌发率差异不显著。在低温冷冻和超低温冷冻条件下桂花花粉活力下降较慢,可用于花粉较长时间的保存。
Abstract
The aim was to research the effects of different storage methods on pollen vitality of Osmanthus fragrans(Thunb.)Lour., and explore the suitable temperature condition for pollen storage of sweet Osmanthus. Taking the pollens of nine sweet osmanthus cultivars of four groups as test material, the pollens were preserved in room temperature, low temperature, freezing and supper-low temperature. The pollen viabilities during different storage time were determined. The results indicated that the pollen germination was well developed in the solid medium composed by sucrose 10%, H3BO3 0.01% and agarose 1%. The germination rate could be calculated after 24 hours cultivating under a constant temperature of 25 ℃. The light and dark showed no significant effect on pollen germination. The ability of pollen germination demonstrateed a significant difference in different storage conditions. Under room temperature(15-20 ℃), O. fragrans pollen could survive after 15-20 days. In low temperature, pollen life can be extended to 50 days. In freezing temperature and supper-low temperature, pollen life can be extended longer, and the most(78%)pollen germination of cultivated O. fragrans showed no significant difference after 100 days. Pollen vitality of O. fragrans decline slower under the condition of freezing temperature and supper-low temperature, it can be preserved for longer.
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基金
收稿日期:2014-09-10
基金项目:江苏省博士创新基金项目; 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:张洪伟,硕士生。*通信作者:王贤荣,教授,博士。E-mail: wangxianrong66@njfu.edu.cn。
引文格式:张洪伟,段一凡,李稚,等. 不同贮藏方法对桂花花粉活力影响的研究[J]. 南京林业大学学报:自然科学版,2014,38(S1):6-12.
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