一株菌根辅助细菌(MHB)芽孢杆菌Bacillus sp.对杨树生长的影响

doi:10.3969/j.issn.1000-2006.2015.01.005

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (01): 22-26.doi: 10.3969/j.issn.1000-2006.2015.01.005

一株菌根辅助细菌(MHB)芽孢杆菌Bacillus sp.对杨树生长的影响

赵柳,吴小芹^*,叶建仁

南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037

出版日期:2015-01-31 发布日期:2015-01-31
基金资助:
收稿日期:2013-12-18 修回日期:2014-03-30
基金项目:国家林业公益性行业科研专项项目(201004061); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:赵柳,硕士。*通信作者:吴小芹,教授。E-mail: xqwu@njfu.edu.cn。
引文格式:赵柳,吴小芹,叶建仁. 一株菌根辅助细菌(MHB)芽孢杆菌Bacillus sp.对杨树生长的影响[J]. 南京林业大学学报:自然科学版,2015,39(1):22-26.

Effect of mycorrhiza helper Bacillus sp. on the growth of poplar seedlings

ZHAO Liu, WU Xiaoqin^*, YE Jianren

Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2015-01-31 Published:2015-01-31

摘要/Abstract

摘要： 为探讨菌根辅助细菌(MHB)对杨树生长的影响,在温室条件下,将筛选到的菌根辅助细菌芽孢杆菌Bacillus sp. DZ18分别与两种外生菌根真菌彩色豆马勃(Pisolithus tinctorius(Pers.)Coker & Couch,Pt2)、劣味乳菇(Lactarius insulsus Fr. Li)双接种于1年生美洲黑杨(Populus deltoides),处理120 d后,测定菌根形成率以及对杨树苗高、地径、根生物量、茎生物量、光合作用、矿物质代谢能力的影响。结果表明,芽孢杆菌DZ18与Pt2、Li双接种时与单接种Pt2、Li相比显著提高了杨树的菌根形成率,且无论单接种DZ18还是DZ18与两种外生菌根真菌双接种均对杨树的各项生长指标具有显著的促进作用。

Abstract: The present greenhouse study was undertaken to evaluate the effects of co-inoculating the ectomycorrhizal(ECM)fungus Pisolithus tinctorius(Pers.)Coker & Couch(Pt2), Lactarius insulsus Fr.(Li)with the mycorrhiza helper bacterium Bacillus sp. DZ18 on the growth of poplar seedlings. After 120 days treatment, ectomycorrhizal colonization rate, shoot length, root crown diameter, root biomass, stem biomass, photosynthesis, and mineral metabolism ability of poplar were measured. The results showed that Bacillus DZ18 coinoculated with Pisolithus tinctorius(Pt2)or Lactarius insulsus(Li)significantly improved mycorrhizal formation rate of poplar compared with a single inoculation Pt2 or Li, and whether single inoculation DZ18 or DZ18 coinoculation with Pt2 and Li, the poplar growth indices were significantly promoted. Therefore, MHB strain DZ18 had potential value to become an excellent bio-fertilizer strain resources.

中图分类号:

S718.83

赵柳,吴小芹,叶建仁. 一株菌根辅助细菌(MHB)芽孢杆菌Bacillus sp.对杨树生长的影响[J]. 南京林业大学学报（自然科学版）, 2015, 39(01): 22-26.

ZHAO Liu, WU Xiaoqin, YE Jianren. Effect of mycorrhiza helper Bacillus sp. on the growth of poplar seedlings[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 39(01): 22-26.DOI: 10.3969/j.issn.1000-2006.2015.01.005.

参考文献

[1] Cripps C, Miller Jr O K. Ectomycorrhizal fungi associated with aspen on three sites in the north-central Rocky Mountains[J]. Canadian Journal of Botany, 1993(14):14-20.
[2] Smith S E, Reid D. Mycorrhizalsymbiosis[M]. London: Academic Press, 1997: 11-16.
[3] Rico’n A, Alvarez I F, Pera J. Inoculation of containerized Pinus pinea L. seedlings with seven ectomycorrhizalfungi[J]. Mycorrhiza,2001(11): 265-271.
[4] Teste F P, Schmidt M G, Berch S M, et al. Effects of ectomycorrhizal inoculants on survival and growth of interior douglas-fir seedlings on reforestation sites and partially rehabilitated landings[J]. Canadian Journal of Forest Research, 2004, 34(10): 2074-2088.
[5] Ortega U, Dunabeitia M, Menedez S, et al. Effectiveness of mycorrhizal inoculation in thenursery on growth and water relations of Pinus radiata in different water regime[J]. Tree Physiology, 2004(24): 65-73.
[6] Godbout C, Fortin J A. Synthesized ectomycorrhizae of aspen: fungal genus level of structural characterization[J]. Canadian Journal of Botany, 1985(63): 252-262.
[7] 宋微, 吴小芹, 叶建仁. 江苏几种杨树优良外生菌根真菌的筛选[J]. 南京林业大学学报:自然科学版, 2009, 33(2): 81-84. Song W, Wu X Q, Ye J R. Screening elite ectomycorrhizal fungi for poplars in Jiangsu[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2009, 33(2): 81-84.
[8] Garbaye J. Mycorrhiza helper bacteria: a new dimension to the mycorrhizalsymbiosis[J]. New Phytol,1994(128): 197-210.
[9] Founoune H, Duponnois R, Meyer J M, et al. Interactions between ectomycorrhizal symbiosis and fluorescent Pseudomonads on Acacia holosericea: isolation of mycorrhization helper bacteria(MHB)from a soudano-sahelian soil[J]. FEMS Microbiology and Ecology, 2002(41): 37-46.
[10] Frey-Klett P, Pierrat J C, Garbaye J. Location and survival of mycorrhizal helper pseudomonas fluorescens during establishment of ectomycorrhizal symbiosis between Laccaria bicolor and Douglas fir[J]. Applied and Environmental Microbiology, 1997(63):139-144.
[11] 盛江梅, 吴小芹. 一株黑松-美味牛肝菌菌根辅助细菌的筛选[J]. 应用与环境生物学报, 2010,16(5): 701-704.Sheng J M, Wu X Q.Isolation and identification of a MHB strain from the rhizosphere soil of Pinus thunbergi inoculated with Boletus edulis[J]. Chin J Appl Environ Biol, 201016(5): 701-704.
[12] Wu X Q, Hou L L, Sheng J M, et al. Effects of ectomycorrhizal fungus Boletus edulis and mycorrhiza helper Bacillus cereus on the growth and nutrient uptake by Pinus thunbergii[J]. Biology and Fertility of Soils, 2012, 48(4): 385-391.
[13] Zhao L, Wu X Q, Ye J R, et al. Isolation and characterization of a mycorrhiza helper bacterium from rhizosphere soils of poplar stands[J]. Biology and Fertility of Soils, 2014, 50(4): 593-601.
[14] Probanza A, Mateos J L, Lucas Garcia J A, et al. Effects of inoculation with PGPR bacillus and Pisolithus tinctorius on Pinus pinea L. growth, bacterial rhizospherecolonialization and mycorrhizalinfection[J]. Microbial Ecology,2001(41): 140-148.
[15] Founoune H, Duponnois R, Ba A M, et al. Mycorrhiza helper bacteria stimulate ectomycorrhizal symbiosis of Acacia holosericea with Pisolithus alba[J]. New Phytologist, 2002, 153(1): 81-89.

一株菌根辅助细菌(MHB)芽孢杆菌Bacillus sp.对杨树生长的影响

Effect of mycorrhiza helper Bacillus sp. on the growth of poplar seedlings

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