为探讨菌根辅助细菌(MHB)对杨树生长的影响,在温室条件下,将筛选到的菌根辅助细菌芽孢杆菌Bacillus sp. DZ18分别与两种外生菌根真菌彩色豆马勃(Pisolithus tinctorius(Pers.)Coker & Couch,Pt2)、劣味乳菇(Lactarius insulsus Fr. Li)双接种于1年生美洲黑杨(Populus deltoides),处理120 d后,测定菌根形成率以及对杨树苗高、地径、根生物量、茎生物量、光合作用、矿物质代谢能力的影响。结果表明,芽孢杆菌DZ18与Pt2、Li双接种时与单接种Pt2、Li相比显著提高了杨树的菌根形成率,且无论单接种DZ18还是DZ18与两种外生菌根真菌双接种均对杨树的各项生长指标具有显著的促进作用。
Abstract
The present greenhouse study was undertaken to evaluate the effects of co-inoculating the ectomycorrhizal(ECM)fungus Pisolithus tinctorius(Pers.)Coker & Couch(Pt2), Lactarius insulsus Fr.(Li)with the mycorrhiza helper bacterium Bacillus sp. DZ18 on the growth of poplar seedlings. After 120 days treatment, ectomycorrhizal colonization rate, shoot length, root crown diameter, root biomass, stem biomass, photosynthesis, and mineral metabolism ability of poplar were measured. The results showed that Bacillus DZ18 coinoculated with Pisolithus tinctorius(Pt2)or Lactarius insulsus(Li)significantly improved mycorrhizal formation rate of poplar compared with a single inoculation Pt2 or Li, and whether single inoculation DZ18 or DZ18 coinoculation with Pt2 and Li, the poplar growth indices were significantly promoted. Therefore, MHB strain DZ18 had potential value to become an excellent bio-fertilizer strain resources.
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基金
收稿日期:2013-12-18 修回日期:2014-03-30
基金项目:国家林业公益性行业科研专项项目(201004061); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:赵柳,硕士。*通信作者:吴小芹,教授。E-mail: xqwu@njfu.edu.cn。
引文格式:赵柳,吴小芹,叶建仁. 一株菌根辅助细菌(MHB)芽孢杆菌Bacillus sp.对杨树生长的影响[J]. 南京林业大学学报:自然科学版,2015,39(1):22-26.
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