拟松材线虫组织蛋白酶基因Bmcath1是一个在松材线虫与拟松材线虫中差异表达的基因,为了解该基因的生物学功能,对Bmcath1基因进行了生物信息学分析。笔者使用BioEdit、SignalP 4.1、BLASTP、MEGA 4. 0等软件对Bmcath1基因进行了生物信息学分析,发现Bmcath1 cDNA序列全长1 294 bp,含有一个长度为1 158 bp的开放阅读框。由Bmcath1基因编码的蛋白质CATH1含有386个氨基酸,分子质量为42.38 ku,等电点为8.52。蛋白质CATH1存在一个Peptidase_C1A subfamily功能域结构,在不同物种上的氨基酸序列多样性丰富。采用Real-Time PCR方法对不同培养方式获得的拟松材线虫中Bmcath1基因的表达量进行检测,发现该基因在分离自黑松的拟松材线虫中高量表达,据此推测拟松材线虫Bmcath1基因可能在线虫处于松树体内生长、繁殖时发挥更为重要的作用。
Abstract
Cathepsin gene Bmcath1 of Bursaphelenchus mucronatus is differentially expressed between B. xylophilus and B. mucronatus. In order to understand its biological function, bioinformational analysis was conducted using software BioEdit, SignalP 4.1, BLASTP and MEGA 4. 0. The result showed that the full-length cDNA of Bmcath1 was 1 294 bp long, which included an ORF of 1 158 bp. The deduced amino sequence of CATH1 contained 386 amino acids with molecular mass of 42.38 ku and pI of 8.52. Protein CATH1 contained a Peptidase-C1A conserved domain. The amino sequences of cathepsins varied between different species. With the method of real-time PCR, gene expression profiling showed that Bmcath1 expressed much more in B. mucronatus separated from Pinus thunbergii seedlings comparing with expression level of Bmcath1 in monoxenic culture nematodes. The result suggested that function of Bmcath1 may be related to infection process of B. mucronatus.
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基金
收稿日期:2014-07-05 修回日期:2015-04-11
基金项目:国家自然科学基金项目(31270683); 江苏高校自然科学研究重大项目(11KJA220002); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:潘妍毅,硕士生。*通信作者:吴小芹,教授。E-mail: xqwu@njf.edu.cn。
引文格式:潘妍毅,黄麟,吴小芹. 拟松材线虫组织蛋白酶基因Bmcath1的序列分析与转录水平检测[J]. 南京林业大学学报:自然科学版,2015,39(6):12-16.
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