榉树枯萎病的发生为害调查及病原鉴定

doi:10.3969/j.issn.1000-2006.2015.06.005

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (06): 24-28.doi: 10.3969/j.issn.1000-2006.2015.06.005

榉树枯萎病的发生为害调查及病原鉴定

刘侃诚¹,许伟¹,郁世军²,李英²,韩正敏^1*

1.南京林业大学林学院,江苏南京 210037;
2.江苏农景生态建设有限公司,江苏句容 212400

出版日期:2015-11-30 发布日期:2015-11-30
基金资助:
收稿日期:2014-06-08 修回日期:2014-10-08
基金项目:江苏省农业科技支撑计划(BE2013409)
第一作者:刘侃诚,博士生。*通信作者:韩正敏,教授。E-mail: zmhan@njfu.edu.cn。
引文格式:刘侃诚,许伟,郁世军,等. 榉树枯萎病的发生为害调查及病原鉴定[J]. 南京林业大学学报:自然科学版,2015,39(6):24-28.

Investigations on blight disease of Zelkova schneideriana and pathogen identification

LIU Kancheng¹,XU Wei¹,YU Shijun²,LI Ying²,HAN Zhengmin^1*

1.College of Forestry,Nanjing Forestry University,Nanjing 210037,China;
2.Jiangsu Nongjing Ecological Construction Co., Ltd., Jurong 212400,China

Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 榉树枯萎病(blight disease of Zelkova schneideriana)是在榉树上发现的一种新的病害,为了解该病害的发生发展,确定该病害的病原,笔者对病害的发生和为害情况进行了实地调查,用柯赫氏法则确定了病原,并对病原进行了形态与分子鉴定。研究结果表明,病害在林地和苗圃均有分布,导致榉树大树和幼苗死亡,榉树地上部分逐渐失绿枯萎,根部变色腐烂。对枯萎病病原进行了分离、接种和再分离,确定该病病原,并通过形态学特性和rDNA-ITS序列分析,鉴定该病害病原为尖孢镰刀菌(Fusarium oxysporum)。对该病的传染性进行分析确定了该病害为土传病害,病害的发生与地形地势有关,低洼地段病害往往发生较重,有随地表水流到处传播特点。病原物尖孢镰刀菌产生大量厚垣孢子,孢子从植物根部入侵,造成根部皮层腐烂,地上部分枯萎。

Abstract: Blight disease of Zelkova schneideriana is a disease found firstly in the Z. schneideriana. In order to investige the blight disease of Z.schneideriana and pathogen identification. The occurrence of the blight disease in Zhenjiang area was investigated. The pathogen was determined by Koch's postulates. The species of the pathogen were identified based on morphological and molecular characteristics. The detailed results are listed as follows: The disease occurs in forest and nursery, and leads to the death of trees and saplings. The symptoms include slow chlorosis of leaves and rot of its roots. The pathogen was identified through isolation,inoculation and re-isolation. After further research on morphologic properties and rDNA-ITS sequences, the pathogen was identified as Fusarium oxysporum. This study also indicates that the disease is a soil-borne diseases, and the occurrence of disease is concerned with topography, and severe disease often occurs in low-lying wet areas in the field. The disease is transmitted through surface water. Large quantities of chlamydospores are produced by F. oxysporum. These spores invade the roots. Then root cortex begins to rot, tree begins to wither.

中图分类号:

S763.15

刘侃诚,许伟,郁世军,等. 榉树枯萎病的发生为害调查及病原鉴定[J]. 南京林业大学学报（自然科学版）, 2015, 58(06): 24-28.

LIU Kancheng,XU Wei,YU Shijun,LI Ying,HAN Zhengmin. Investigations on blight disease of Zelkova schneideriana and pathogen identification[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(06): 24-28.DOI: 10.3969/j.issn.1000-2006.2015.06.005.

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榉树枯萎病的发生为害调查及病原鉴定

Investigations on blight disease of Zelkova schneideriana and pathogen identification

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