间歇出酶对里氏木霉产纤维素酶的影响

doi:10.3969/j.issn.1000-2006.2016.01.014

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (01): 87-91.doi: 10.3969/j.issn.1000-2006.2016.01.014

间歇出酶对里氏木霉产纤维素酶的影响

白合超,蒋露,王步成,勇强,余世袁^*

南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京 210037

出版日期:2016-02-18 发布日期:2016-02-18
基金资助:
收稿日期:2015-02-15 修回日期:2015-07-08
基金项目:“十二五”国家科技支撑计划(2015BAD15B09)
第一作者:白合超(645233834@qq.com)。*通信作者:余世袁(syu@njfu.edu.cn),教授。
引文格式:白合超,蒋露,王步成,等. 间歇出酶对里氏木霉产纤维素酶的影响[J]. 南京林业大学学报(自然科学版),2016,40(1):87-91.

Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei

BAI Hechao, JIANG Lu, WANG Bucheng, YONG Qiang,YU Shiyuan^*

Key Laboratory of Forest Genetics &
Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Online:2016-02-18 Published:2016-02-18

摘要/Abstract

摘要： 在分批补料的基础上,采用间歇出酶的方法,对里氏木霉产纤维素酶进行了研究。结果表明:分批补料过程中最佳的补料速度为5.5 g/(L·d),在此条件下产酶第8天滤纸酶活力和β-葡萄糖苷酶活力达到14.40 U/mL和1.59 U/mL。在分批补料的基础上进行间歇出酶,与对照相比,第4、6、8天出酶模式(模式1)时,总滤纸酶活力提高18.14%; 第4、7天出酶模式(模式2)时,总滤纸酶活力提高17.75%; 第5、8天出酶模式(模式3)时,总滤纸酶活力提高27.35%。研究表明,通过间歇出酶可以有效提高纤维素酶总滤纸酶的活力。

Abstract: This paper investigated fed-batch and periodic harvest of enzyme for cellulase production by Trichoderma reesei. The results showed that the best feeding rate was 5.5 g/(L·d)cellulose per day, and the filter paper activity and β-glucosidase were 14.40 U/mL and 1.59 U/mL after 8 days of cultivation. Based on fed-batch, when harvesting enzyme at the 4^th, 6^th, 8^th day, the total filter paper activity improved by 18.14%. When harvesting at the 4^th,7^th day, the total filter paper activity improved by 17.75%. When harvesting at the 5^th,8^th day, the total filter paper activity improved by 27.35%.This paper concluded that periodic harvest of enzyme could improve the total filter paper activity efficiently.

中图分类号:

TQ351
Q814

白合超,蒋露,王步成,等. 间歇出酶对里氏木霉产纤维素酶的影响[J]. 南京林业大学学报（自然科学版）, 2016, 40(01): 87-91.

BAI Hechao, JIANG Lu, WANG Bucheng, YONG Qiang,YU Shiyuan. Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(01): 87-91.DOI: 10.3969/j.issn.1000-2006.2016.01.014.

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间歇出酶对里氏木霉产纤维素酶的影响

Effect of periodic harvest of enzyme on cellulase production by Trichoderma reesei

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