吡咯伯克霍尔德氏菌JK-SH007分批发酵
动力学模型的构建

doi:10.3969/j.issn.1000-2006.2016.02.029

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (02): 173-178.doi: 10.3969/j.issn.1000-2006.2016.02.029

吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建

李浩^{1, 2},任嘉红^{1, 3},叶建仁^1*,王小月¹

1.南京林业大学林学院,南京林业大学南方现代林业协同创新中心,江苏南京 210037;
2.中国科学院合肥物质科学研究院医学物理与技术中心,安徽合肥 230031;
3.长治学院生物科学与技术系,山西长治 046011

出版日期:2016-04-18 发布日期:2016-04-18
基金资助:
收稿日期:2014-11-26 修回日期:2015-12-26
基金项目:国家林业公益性行业科研专项项目(201304404); 江苏高校优势学科建设工程资助项目(PAPD); 国家自然科学基金项目(31270684,31470645)
第一作者:李浩(lihaonfu@163.com)。*通信作者:叶建仁(jrye@njfu.edu.cn),教授。
引文格式:李浩,任嘉红,叶建仁,等. 吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建[J]. 南京林业大学学报(自然科学版),2016,40(2):173-178.

Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007

LI Hao^1,2, REN Jiahong^1,3, YE Jianren^1*, WANG Xiaoyue¹

1.College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2. Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sc

Online:2016-04-18 Published:2016-04-18

摘要/Abstract

摘要： 吡咯伯克霍尔德氏菌JK-SH007是分离自杨树中的一种细菌,其对杨树病原真菌具有抑制作用,是一种具有生防应用潜力的有益菌株。为深入了解该菌株的发酵进程,放大发酵规模,并对发酵过程进行最优化控制,笔者对JK-SH007菌株液体分批发酵产生抑菌活性的动力学进行了研究。根据发酵实验数据,分别选用Logistic 方程、Luedeking-Piret 方程和Luedeking-Piret like方程建立了模拟菌体生长、抑制真菌活性产生和葡萄糖消耗的动力学模型。经拟合分析发现,动力学模型的计算值与实验值拟合良好,可直观反映JK-SH007菌株分批发酵过程中的动力学特征。

Abstract: Burkholderia pyrrocinia JK-SH007 isolated from poplar can inhibit the pathogenic fungi in poplar. To optimum fermentation process of strain JK-SH007, fermentation kinetics were investigated in this study. A kinetic model for the simulation of bacterial growth, the inhibition of fungal activity generation and glucose consumption were constructed by using Logistic equation, Luedeking-Piret equation and Luedeking-Piret like equation, respectively, based on the data from fermentation experiment. The calculated values in kinetic models fit well with experimental values which directly reflected the kinetic characteristics during the batch fermentation process of strain JK-SH007. Moreover, the fermentation kinetics model provided a foundation for the optimization and amplification of fermentation process.

中图分类号:

Q939.95

李浩,任嘉红,叶建仁,等. 吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建[J]. 南京林业大学学报（自然科学版）, 2016, 40(02): 173-178.

LI Hao, REN Jiahong, YE Jianren, WANG Xiaoyue. Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(02): 173-178.DOI: 10.3969/j.issn.1000-2006.2016.02.029.

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吡咯伯克霍尔德氏菌JK-SH007分批发酵动力学模型的构建

Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007

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Construction of batch fermentation kinetics model of Burkholderia pyrrocinia strain JK-SH007

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