短丝木犀转录组测序及类胡萝卜素生物合成相关基因表达分析

陈林; 李龙娜; 戴亚平; 杨国栋

doi:10.3969/j.issn.1000-2006.2016.05.004

陈林,李龙娜,戴亚平,杨国栋

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (05) : 21-28.

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (05) : 21-28. DOI: 10.3969/j.issn.1000-2006.2016.05.004

研究论文

短丝木犀转录组测序及类胡萝卜素生物合成相关基因表达分析

陈林¹,李龙娜²,戴亚平¹,杨国栋¹

作者信息 +

De novo transcriptome sequencing and analysis of carotenoids biosynthesis related gene expression in Osmanthus serrulatus

CHEN Lin¹, LI Longna², DAI Yaping¹, YANG Guodong¹

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文章历史 +

摘要

短丝木犀(Osmanthus serrulatus)是我国特有的木犀属香花树种,具有极高的开发应用前景。笔者以短丝木犀为研究材料,应用Illumina HISeq^TM2000高通量测序平台,对短丝木犀的花和叶芽进行转录组深度测序和拼接组装,构建和预测了短丝木犀不同组织间的基因表达谱及其代谢通路。转录组测序共获得16.94 G的有效数据,经Trinity从头组装得到 92 798条单基因簇(unigenes),其中35 851条unigenes与Nr、Nt、Pfam、KOG、Swiss-Prot、KEGG、PFAM等7大公共数据库比对获得了基因功能注释信息。由KEGG 代谢通路分析发现,共有8 779条unigenes参与到262个代谢通路中,其中参与到色素和香味代谢的基因分别有53条和335条。此外,针对6条在短丝木犀花和叶芽间显著差异表达的参与类胡萝卜素生物合成的相关基因,通过实时荧光定量PCR,验证了它们在不同组织间的表达模式。

Abstract

Osmanthus serrulatus is an endemic species of China with high potentiality in the practical application. To investigate the transcriptome profiling of this species, a high throughput RNA sequencing via Illumina HISeq^TM2000 platforms was applied on the flowers and leaf buds of O. serrulatus. Amounting to 16.94 G of clean reads was generated from the sequencing, and then was de novo assembled via trinity. The draft transcriptome assembly consisted of 92 798 unigenes, of which 35 851 unigenes were successfully annotated against the following public databases: Nr, Nt, Swiss-Prot, GO, COG, KEGG and PFAM. The KEGG results showed that a total of 8 779 unigenes could be mapped onto 262 pathways, among which 53 and 335 unigenes involved in the biosynthesis of floral pigment and of fragrance, respectively. In addition, differential gene expression patterns between flowers and leaf buds of O. serrulatus were confirmed by the results of qRT-PCR on 6 genes that are related to the biosynthesis of carotenoid. Our study provides a valuable resource for future studies aimed at identifying the molecular mechanisms of synthesizing flora pigment and fragrance on O. serrulatus.

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陈林,李龙娜,戴亚平,杨国栋. 短丝木犀转录组测序及类胡萝卜素生物合成相关基因表达分析[J]. 南京林业大学学报（自然科学版）. 2016, 40(05): 21-28 https://doi.org/10.3969/j.issn.1000-2006.2016.05.004

CHEN Lin, LI Longna, DAI Yaping, YANG Guodong. De novo transcriptome sequencing and analysis of carotenoids biosynthesis related gene expression in Osmanthus serrulatus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(05): 21-28 https://doi.org/10.3969/j.issn.1000-2006.2016.05.004

中图分类号： S685.13 Q781

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基金

收稿日期:2015-10-14 修回日期:2016-02-25
基金项目:国家自然科学基金项目(31300558); 江苏省基础研究计划项目(BK20130972); 江苏高校优势学科建设工程资助项目(PAPD); 南京林业大学高学历人才基金项目(GXL201308)
第一作者:陈林(clinechen@njfu.edu.cn),主要负责实验设计、执行及论文修改; 李龙娜(lln2013034@njau.edu.cn),主要负责数据分析和论文初稿写作。
引文格式:陈林,李龙娜,戴亚平,等. 短丝木犀转录组测序及类胡萝卜素生物合成相关基因表达分析[J]. 南京林业大学学报(自然科学版),2016,40(5):21-28.