基于SCAR标记的20个樱花品种的分子鉴别

doi:10.3969/j.issn.1000-2006.2016.05.006

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (05): 34-40.doi: 10.3969/j.issn.1000-2006.2016.05.006

基于SCAR标记的20个樱花品种的分子鉴别

赵庆杰^1,2,李海波¹, 屈燕²,王青华³, 郭佳⁴,徐梁^{1 Symbolj@@}

1.浙江省林业科学研究院, 浙江省森林资源生物与化学利用重点实验室,浙江杭州 310023;
2.西南林业大学园林学院,云南昆明 650224;
3.武汉东湖生态旅游风景区,湖北武汉 430074;
4.浙江诚邦园林股份有限公司,浙江杭州 310008

出版日期:2016-10-18 发布日期:2016-10-18
基金资助:
收稿日期:2015-09-01 修回日期:2016-05-09
基金项目:浙江省花卉新品种选育重大科技专项重点项目(2012C12909-5); 浙江省科研院所扶持专项项目(2013F10020,2014F30002,2016F30013); 浙江省林业科研成果推广项目(2013B08); 杭州市科技项目(20130932H11)
第一作者:赵庆杰(1018799912@qq.com)。*通信作者:徐梁(123298232@qq.com),助理研究员。
引文格式:赵庆杰,李海波, 屈燕,等. 基于SCAR标记的20个樱花品种的分子鉴别[J]. 南京林业大学学报(自然科学版),2016,40(5):34-40.

SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars

ZHAO Qingjie^1,2, LI Haibo¹, QU Yan², WANG Qinghua³, GUO Jia⁴, XU Liang^{1 Symbolj@@}

1.Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023, China;
2. School of Landscape, Southwest Forestry University, Kunming 650224, China;
3. East Lake Scenic Area of Wuhan, Wuhan 430074, China;
4. Zhejiang Chengbang Landscape Architecture Company, Hangzhou 310008, China

Online:2016-10-18 Published:2016-10-18

摘要/Abstract

摘要： 以20个广泛栽培与应用的樱花品种为试材,基于特异SCAR分子标记的开发鉴别樱花品种。结果表明:4条SAPD引物和96对SRAP引物分别在20个樱花品种中的扩增得到了33条nSAPD多态片段和13条SRAP多态性片段,其中10条nSAPD和4条SRAP多态片段被成功转化为14个稳定可靠的特异SCAR标记。这14个SCAR标记在20个樱花品种中的分布不同,其中,‘普贤象'、‘松月'、‘御衣黄'、‘关山'、‘大提灯'和‘旭山樱'只有1个SCAR标记,‘郁金'、‘红丰'、‘咲耶姬'、‘朱雀'和‘菊垂樱'有2个SCAR标记,‘市原虎之尾'和‘平野妹背'有3个SCAR标记,‘八重红枝垂'和‘雨情枝垂'有4个SCAR标记,‘红笠'、‘红华'、‘一叶'、‘福禄寿'和‘杨贵妃'则没有SCAR标记。这些SCAR标记可作为品种特异性的DNA指纹,辅助用于20个樱花品种的鉴别,也可用于分子水平建立樱花品种分类鉴定系统。

Abstract: In the present study, 20 cultivars of flowering cherries(Cerasus), which were widely cultivated in gardens, were identified by developing specific sequence characterized amplified region(SCAR)markers from amplified polymorphic DNA(SAPD)and sequence-related amplified polymorphism(SRAP)techniques. Forty-six polymorphic bands(33 from SAPD and 13 from SRAP)among the 20 studied cultivars were obtained by using 4 SAPD primers and 96 SRAP primers. Fourteen(10 from SAPD and 4 from SRAP)of 46 polymorphic bands were converted into 14 stable and reproducible SCAR markers by designing specific SCAR primers from these sequenced polymorphic bands and by verifying PCR amplification with SCAR primers. The 14 SCAR markers was distributed in 20 different Cerasus cultivars. The cultivars C. serrulata var. lannesiana ‘Albo-rosea', C. serrulata var. lannesiana ‘Superba', C. serrulata var. lannesiana ‘Gioiko', C. serrulata var. lannesiana ‘Kanzan', C. serrulata var. lannesiana ‘Ojochin' and C. serrulata var. lannesiana ‘Asahiyama' possess only one SCAR marker, C.serrulata var. lannesiana ‘Grandifora', Cerasus × sieboldii ‘Beni-yutaka', Cerasus × yedoensis ‘Sakuyahime', C.serrulata var. lannesiana ‘Shujaku' and C. serrulata var. lannesiana ‘Plena-pendula' possess two SCAR markers, C. jamasakura ‘Albo Plena' and C. serrulata var. lannesiana ‘Imose' possess three SCAR markers, C. subhirtella ‘Plena Rosea' and C.subhirtella‘Ujou-shidare' possess four SCAR markers, and C. serrulata var. lannesiana ‘Benigasa', C. serrulata var. lannesiana ‘Kouka', C.serrulata var. lannesiana ‘Hisakura', C. serrulata var. lannesiana ‘Contorta' and C. serrulata var. lannesiana‘Mollis'possess no SCAR markers. These SCAR markers can be used as cultivar-specific DNA fingerprints to promote the identification of 20 Cerasus cultivars, as well as privide molecular data for the establishment of identification system of Cerasus cultivars and for the further study of Cerasus germplasm.

中图分类号:

S685

赵庆杰,李海波,屈燕,等. 基于SCAR标记的20个樱花品种的分子鉴别[J]. 南京林业大学学报（自然科学版）, 2016, 40(05): 34-40.

ZHAO Qingjie, LI Haibo, QU Yan, WANG Qinghua, GUO Jia, XU Liang^{1 Symbolj@@}. SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(05): 34-40.DOI: 10.3969/j.issn.1000-2006.2016.05.006.

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基于SCAR标记的20个樱花品种的分子鉴别

SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars

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