南京林业大学学报(自然科学版) ›› 2020, Vol. 44 ›› Issue (4): 119-124.doi: 10.3969/j.issn.1000-2006.201912030

• 研究论文 • 上一篇    下一篇

盐胁迫下丛枝菌根真菌对桢楠根系生长和激素的影响

崔令军(), 刘瑜霞, 林健, 石开明()   

  1. 生物资源保护与利用湖北省重点实验室,湖北民族大学,湖北 恩施,445000
  • 收稿日期:2019-12-18 修回日期:2020-03-04 出版日期:2020-07-22 发布日期:2021-07-09
  • 通讯作者: 石开明
  • 作者简介:崔令军(lingjuncui@163.com)
  • 基金资助:
    生物资源保护与利用湖北省重点实验室开放基金(PKLHB2007);湖北省“双一流”建设专项资金;湖北民族大学博士启动基金(MY2017B029)

Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress

CUI Lingjun(), LIU Yuxia, LIN Jian, SHI Kaiming()   

  1. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, China
  • Received:2019-12-18 Revised:2020-03-04 Online:2020-07-22 Published:2021-07-09
  • Contact: SHI Kaiming

摘要: 目的

研究不同浓度盐胁迫下,桢楠接种丛枝菌根真菌(arbuscular mycorrhizal fungi, AMF)后根系形态结构和激素含量及其动态变化情况,为桢楠在盐碱地造林提供技术支撑。

方法

采用爱普生Perfection V700双镜头系统扫描整个根系并用Winrhizo软件进行分析,菌根侵染率采用根段频率标准法测定,激素含量测定采用酶联免疫吸附测定法(ELISA)。

结果

① AMF接种处理显著促进了桢楠根系的生长,在NaCl浓度为300 mmol/L时,AMF接种处理根系总长度、表面积、体积和侧根数分别比未接种处理高21. 11%、40. 98%、18. 71%和32. 58%, ②随着NaCl浓度的增加,AMF接种与未接种处理桢楠根系GA3、IAA、ZR含量均呈下降趋势,ABA含量则呈上升趋势。③ AMF接种处理显著降低了根系激素的比值,在NaCl浓度为300 mmol/L时,AMF接种处理的根系m(ABA)/m(GA3) (ABA/ GA3,下同)、ABA/ IAA、ABA/ ZR、ABA/(GA3+IAA+ZR)分别比未接种处理降低18. 55%、28. 13%、89. 91%和24. 74%。

结论

在不同盐浓度胁迫下,AMF显著提高了桢楠根系的总长度、表面积、体积和侧根数量,打破激素间的平衡,增加促进根系生长的激素GA3、IAA、ZR含量,降低抑制根系生长的激素ABA含量,表明AMF能够增强桢楠的耐盐性,进而促进根系生长。

关键词: 桢楠, 丛枝菌根真菌, 盐胁迫, 激素

Abstract: Objective

The combined effects of arbuscular mycorrhizal fungi (AMF) and salt stress on the morphological structure, endogenous hormone content, and dynamic changes in Phoebe zhennan were studied to provide a theoretical support for P. zhennan afforestation in saline-alkali conditions.

Method

An Epson Perfection V700 dual-lens system was used to scan the full root system. An analysis was performed using Winrhizo software; the mycorrhizal infection rates were determined by the standard method of root segment frequency, and the endogenous hormone content and dynamic changes were determined by enzyme-linked immunosorbent assay (ELISA).

Result

The NaCl concentration was 300 mmol/L, the total length, surface area, volume and number of lateral roots following the AMF treatment were 21.11%, 40.98%, 18.71% and 32.58% higher than those of the non-AMF treatment, respectively; and AMF treatment significantly increased the growth of the root system. With NaCl concentrations increasing, the contents of GA3, IAA and ZR in P. zhennan roots treated with AMF decreased, but the ABA content increased. The NaCl concentration was 300 mmol/L, and the mass ratios of ABA to GA3, ABA to IAA, ABA to ZR, ABA to (GA3 +IAA +ZR) following the AMF treatment were 18.55%, 28.13%, 89.91% and 24.74% lower than those of the non-AMF treatment, respectively; the AMF treatment significantly reduced the root hormone ratio.

Conclusion

In P. zhennan plants under varying degrees of salt stress, AMF treatment increased the total length, surface area, volume and number of lateral roots, altered the hormonal balance, increased the contents of GA3, IAA and ZR, and reduced the content of ABA, indicating that AMF can enhance the salt tolerance of P. zhennan and promote root growth.

Key words: Phoebe zhennan, arbuscular mycorrhizal fungi(AMF), salt stress, endogenous hormone

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