南京林业大学学报(自然科学版) ›› 1993, Vol. 17 ›› Issue (04): 57-61.doi: 10.3969/j.jssn.1000-2006.1993.04.012

• 研究论文 • 上一篇    下一篇

马尾松染色体的银染与G带复合技术的探索

陈天华;徐进;赖焕林   

  1. 南京林业大学林学系;南京林业大学林学系;南京林业大学林学系
  • 出版日期:1993-12-18 发布日期:1993-08-18

AN APPROACH TO THE DISPLAYING SILVERSTAINED NUCLEOLUS ORGANIZES REGION AND GIMESA BANDING TECHNIQUE IN MASSON PINE CHROMOSOME

Chen Tianhua Xu jing Lai Huanlin   

  1. Department of Forestry
  • Online:1993-12-18 Published:1993-08-18

摘要: <正>首次报道了马尾松(P.massoniana)染色体银染和G带复合分带的成功,在同一细胞内同时显示出染色体的Ag-NOR和G带。通过计算机扫描,每个染色体的带型清晰。该方法制作简易,效果良好。对植物染色体G带的分带条件等问题进行了初步讨论。

Abstract: The displaying silverstained Ag-NOR and G-Banding technique was first reported in massoniana Lamb. Chromosomal Ag-NOR and G-Band were displayed simultaneously in one cell. G-Bahds were clearly shown with the help of computer-scanning. The technique reported is easy to use with cogent results. Some problems about requirements for G-Banding technique were also discussed.