诱导杨树结瘤固氮的研究

韩素芬;张福海;邓振宇

南京林业大学学报(自然科学版) ›› 1999, Vol. 23 ›› Issue (03) : 42-45.

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南京林业大学学报(自然科学版) ›› 1999, Vol. 23 ›› Issue (03) : 42-45. DOI: 10.3969/j.jssn.1000-2006.1999.03.012
研究论文

诱导杨树结瘤固氮的研究

  • 韩素芬;张福海;邓振宇
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Studies on Inducing Poplar to Nodulate and Fix Nitrogen

  • Author Han SufengZhang FuhaiDeng Zhenyu
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摘要

<正>6种植物激素(2,4-D、NAA、IAA、IBA、6-BA、S.L.)用不同浓度处理杨树无性系NL-80106组培苗根系,其中2,4-D、IBA、S.L.有不同程度的诱瘤作用。以2,4-D和IBA诱导结瘤最为稳定有效,2,4-D诱瘤的最适浓度1.0~1.5mg/L,IBA为2.5~3.0mg/L。分离、回接和显微镜观察表明根瘤菌在诱瘤剂作用下已侵入诱导瘤。诱瘤剂在接种根瘤菌情况下对苗木生长有较为明显的促进作用,无论株高、鲜重和叶绿素含量都高于对照,尤以IBA(3.0mg/L)最为显著。

Abstract

When the roots of tissue culture seedlings of Poplar clone NL_80106 are treated with six plant hormones (2,4-D,NAA,IAA,IBA,6-BA,S.L.)in different concentrations, inducing nodulation is observed in seedlings treated with 2,4-D,IBA and S.L..2,4-D and IBA are the stablest and most effective. The suitable concentrations of 2,4-D and IBA are separately 1.01.5 mg/L and 2.53.0 mg/L in inducing nodulation. Isolation, reciprocal inculation and microscope observation indicate that inducement nodules have been intected by rhizobia. Inductors obviously promote growth of seedlings when inoculating rhizobium on seedlings. The height growth, fresh weight and chlorophyll content of tested seedlings exceed those of contrast seedlings, specially in IBA concentration of 3.0 mg/L.

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韩素芬;张福海;邓振宇. 诱导杨树结瘤固氮的研究[J]. 南京林业大学学报(自然科学版). 1999, 23(03): 42-45 https://doi.org/10.3969/j.jssn.1000-2006.1999.03.012
Author Han SufengZhang FuhaiDeng Zhenyu. Studies on Inducing Poplar to Nodulate and Fix Nitrogen[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 1999, 23(03): 42-45 https://doi.org/10.3969/j.jssn.1000-2006.1999.03.012

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