In order to isolate target genes for controlling the wood formation and quality components, a cDNA library was constructed from Cuninghamia lanceolata stems cambium tissue with the ZAP express phage vector. The host bacteria is E. coli XL1-Blue, and subclone bacteria is E. coli XLOLR. The compacity of primary library is 6.1 X l0s pfu, and the titer of amplified library is 5.2×109 pfu/mL, in which 95% of phages were recombinant, the size of fragments inserted rang from 0.5 kb to 3.0 kb.
YANG Li-wei, SHI Ji-sen*.
Construction, of cDNA Library from Stem Cambium Tissue of Cuninghamia lanceolata (Lam.) Hook[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2005, 29(06): 85-87 https://doi.org/10.3969/j.jssn.1000-2006.2005.06.021