利用Giemsa C-带和染色体荧光原位杂交(45S rDNA FISH)的方法对‘Royal Lace’בHigh Class’的杂种后代分别进行了鉴定。结果表明：‘Royal Lace’的染色体数2n=3x=36，‘High Class’的染色体数2n=2x=24。杂种后代的染色体数出现2n=3x=36和2n=4x=48两种类型。经Giemsa C-带和45S rDNA FISH方法对两个亲本和具2n=4x=48的杂种后代分析结果表明，杂种后代的根尖染色体C-带可观察到来自双亲的可以特异追踪的染色体带纹。FISH分析表明，杂种后代中分别有两条来自‘Royal Lace’和‘High Class’的染色体。两个亲本的荧光原位杂交点数分别为10和9。杂种后代染色体为2n=36的个体有14个杂交信号，可以确定两条来自‘Royal Lace’，另外3条来自‘High Class’。杂种后代中2n=48的个体的杂交信号点为19，从而证实所获得的杂种后代的真实性。同时，对于2n=48，而杂交信号为19 的多倍体起源于未减数分裂的2n雄配子体的可能性进行了探讨。综合研究结果表明，Giemsa C-带和45S rDNA FISH方法可以准确地对百合的杂种真实性进行鉴定。
Abstract
In this study, hybrid plants from the crosses between ‘Royal Lace’ × ‘High Class’ were analyzed with Giemsa C-banding and 45S rDNA FISH. The results showed that, the number of chromosomes was 2n=3x=36 for ‘Royal Lace’, 2n=2x=24 for ‘High Class’ and 2n=3x=36 or 2n=4x=48 for two different hybrids, respectively. The special C-banging patterns of two parents can be found in their hybrids. By FISH analysis, 14 hybridization locies were detected in the hybrids of 2n=36. Two chromosomes from ‘Royal Lace’ and three chromosomes from ‘High Class’ can be identified clearly. And there were 19 hybridization locies to be distinguished for 2n=48 hybrids，which also suggested that these hybrids might relate to be the contributed by unreduced male gametes (2n). All of these evidences clearly confirmed that, the plants were true hybrids.
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基金
收稿日期：2009-02-26修回日期：2009-05-10基金项目：国家林业局“948”创新项目(2007-4-06)；上海农业科技攻关项目(2006-4-1)作者简介：胡凤荣(1969—)，高级工程师，博士。*施季森(通讯作者)，教授，研究方向为林木遗传育种、林木分子生物学。Email: jshi@njfu.edu.cn。引文格式：胡凤荣,刘光欣,罗凤霞,等. 利用Giemsa C-带和45S rDNA FISH的方法鉴定百合杂种[J]. 南京林业大学学报:自然科学版,2009,33(6):30-34.
