Abstract: In this study, hybrid plants from the crosses between ‘Royal Lace’ × ‘High Class’ were analyzed with Giemsa C-banding and 45S rDNA FISH. The results showed that, the number of chromosomes was 2n=3x=36 for ‘Royal Lace’, 2n=2x=24 for ‘High Class’ and 2n=3x=36 or 2n=4x=48 for two different hybrids, respectively. The special C-banging patterns of two parents can be found in their hybrids. By FISH analysis, 14 hybridization locies were detected in the hybrids of 2n=36. Two chromosomes from ‘Royal Lace’ and three chromosomes from ‘High Class’ can be identified clearly. And there were 19 hybridization locies to be distinguished for 2n=48 hybrids，which also suggested that these hybrids might relate to be the contributed by unreduced male gametes (2n). All of these evidences clearly confirmed that, the plants were true hybrids.