单分散荧光微球的制备及其光学性能研究

doi:10.3969/j.jssn.1000-2006.2010.03.004

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (03): 15-19.doi: 10.3969/j.jssn.1000-2006.2010.03.004

单分散荧光微球的制备及其光学性能研究

吴伟兵¹,王明亮²,景宜¹,戴红旗¹

1.南京林业大学轻工科学与工程学院，江苏省制浆造纸科学与技术重点实验室，江苏南京210037; 2.东南大学化学化工学院，江苏南京211189

出版日期:2010-06-29 发布日期:2010-06-29
基金资助:
收稿日期：2009-09-14修回日期：2009-12-24基金项目：江苏省制浆造纸科学与技术重点实验室开放基金课题(200907)作者简介：吴伟兵(1982—),讲师,博士。Email: wbwu@yahoo.cn。引文格式：吴伟兵,王明亮,景宜,等.

Preparation of monodisperse fluorescent microspheres and their optical properties

WU Weibing¹, WANG Mingliang², JING Yi¹, DAI Hongqi¹

1.Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China; 2.School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China

Online:2010-06-29 Published:2010-06-29

摘要/Abstract

摘要： 采用两步分散聚合法通过包覆负载染料的方式制备了单分散聚苯乙烯荧光微球。以高分子质量(Mw=300 000 g/mol)的聚乙烯吡咯烷酮作稳定剂,采用间断式连续滴加染料单体的方式进行制备。该方法制备的荧光微球单分散性好(标准偏差<7 %),染料负载效率高。扫描电镜显示微球粒径均匀，表面形态光滑均匀，无明显缺陷。通过比较发现在微球球壳的保护作用下，染料可有效避免荧光猝灭，荧光信号比同浓度的溶液高几个数量级；此外,微球荧光发射稳定，不受外界环境介质变化的影响。

Abstract: Fluorescent monodisperse polystyrene microspheres were prepared by dispersion polymerization by modified two stage dispersion polymerization in which polyvinylpyrrolidone with high molecular weight (Mw=300 000 g/mol) was used as the stabilizer and the dye was added in a relative long period after nucleation stage. Two stage dispersion polymerization combined with embedment method resulted in more uniform microspheres and higher dye concentration compared to adsorption and conventional dispersion polymerization method. The surface morphology, average diameter, size distribution and photoluminescence properties of microspheres were investigated. The found intense fluorescence and fluorescent resistance to the solvents indicate that the microsphere structure protects dye molecules from fluorescence quenching and ambient medium. This type of monodisperse fluorescent microspheres has potential application in bioassays.

中图分类号:

O632
TB34

吴伟兵,王明亮,景宜,等. 单分散荧光微球的制备及其光学性能研究[J]. 南京林业大学学报（自然科学版）, 2010, 34(03): 15-19.

WU Weibing, WANG Mingliang, JING Yi, DAI Hongqi. Preparation of monodisperse fluorescent microspheres and their optical properties[J].Journal of Nanjing Forestry University (Natural Science Edition), 2010, 34(03): 15-19.DOI: 10.3969/j.jssn.1000-2006.2010.03.004.

参考文献

[1]Wolf S F, Haines L. Rapid hybridization kinetics of DNA attached to submicron latex particles[J]. Nucleic Acids Res, 1987, 15: 2911-2915.
[2]Flynn M A, Odovotz Y V, Kornowskir R, et al. Use of fluorescent microspheres to localize in vivo gene transfer injection sites[J]. Biotechniques, 2000, 28: 470-476.
[3]Finger V H, GuoHaihong, Lu Zhaohai, et al. Expression of chemokine receptors by endothelial cells: detection by intravital microscopy using chemokinelocated fluorescent microspheres[J]. Methods Enzymol, 1997, 288: 148-158.
[4]Yang C S, Chang C H, Tsai P J, et al. Nanoparticlebased in vivo investigation on bloodbrain barrier permeability following ischemia and reperfusion[J]. Anal Chem, 2004, 76: 4465-4471.
[5]Ugelstad J, Mrk P C, Kaggerud K H, et al. Swelling of oligomerpolymer particles. New methods of preparation[J]. Adv Coll Interfac Sci, 1980, 13: 101-140.
[6]Chandler M B, Chandler D J. Microparticles attached to nanoparticles labeled with fluorescent dye US Patent, 6268222[P]. 2001.
[7]Singer V L, Haugland R P. Specific binding assays using fluorescent microparticlescontg.cascade of fluorescent dyes giving high Stokes shift, for detecting wide variety of materials, e.g.proteins, nucleotide(s) and glycolipid(s) US Patent, 5573909[P]. 1996.
[8]Begum Elmas, Murvet Tuncel, Gamze Yalcm, et al. Synthesis of uniform, fluorescent poly(glycidyl metharylate) based particles and their characterization by confocal laser scanning microscopy[J]. Colloids and Surfaces A, 2005, 269: 125-134.
[9]Bosma G, Pathmamanoharan C, de Hoog E, et al. Preparation of monodisperse, fluorescent PMMALatex Colloids by dispersion Polymerization[J]. J Colloid Interface Sci, 2002, 245: 292-300.
[10]Hua Hu, Ronald G Larson. Preparation of fluorescent particles with long excitation and emission wavelengths dispersible in organic solvents[J]. Langmuir, 2004, 20: 7436-7443.
[11]Ugelstad J, Mrk P C, Kaggerud K H, et al. Emulsion Polymerization[M]. New York: Academic, 1982.
[12]Bradford E B, Vanderhoff J W. Electron microscopy of monodisperse latexes[J]. J Appl Phys, 1955, 26: 864-871.
[13]Barrett K E J. Dispersion Polymerization in Organic Media[M]. New York: Wiley, 1975.
[14]Paine A J, Luymes W, McNulty J. Dispersion polymerization of styrene in polar solvents[J]. Macromolecules, 1990, 23: 3104-3109.
[15]Winnik F M, Ober C K. Colored particles by dispersion polymerization[J]. Eur Polym J, 1987, 23: 617-622.
[16]Song J S, Tronc F, Winnik M A. Twostage dispersion polymerization toward monodisperse, controlled micrometersized copolymer particles[J]. J Am Chem Soc, 2004, 126: 6562-6563.
[17]Song J S, Tronc F, Winnik M A. Monodisperse, controlled micronsize dyelabeled polystyrene particles by twostage dispersion polymerization[J]. Polymer, 2006, 47: 817-825.
[18]Lacroixdesmazes P, Guillot J. Dispersion polymerization of styrene in ethanolwater media: monomer partitioning behavior and locus of polymerization[J]. J Polym Sci Part B: Polym Phys, 1998, 36: 325-335.
[19]Kinnunen P K J, Tulkki AP, Lemmetyinen H, et al. Characteristics of excimer formation in Langmuir Blodgett assemblies of 1palmitoyl2pyrenedecanoylphosphatidylcholine and dipalmitoylphosphatidylcholine[J]. Chem Phys Lett, 1987, 136: 539-545.
[20]Grabowski Z R, Rotkiewicz K. Structural changes accompanying intramolecular electron transfer: focus on twisted intramolecular chargetransfer states and structures[J]. Chem Rev, 2003, 103: 3899-4031.
[21]Wittmershaus B P, Skibicki J J, McLafferty J B, et al. Spectral properties of single BODIPY dyes in polystyrene microspheres and in solutions[J]. J Fluoresc, 2001, 11: 119-128.

单分散荧光微球的制备及其光学性能研究

Preparation of monodisperse fluorescent microspheres and their optical properties

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

作者

读者

审稿