松材线虫携带的荧光假单胞菌Pseudomonas fluorescens GcM5-1A菌株分泌的毒素及其对黑松的毒性

doi:10.3969/j.jssn.1000-2006.2010.03.039

.青岛大学生物系，山东青岛0；.南京林业大学森林资源与环境学院，江苏南京00

南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (03) : 179-180.

PDF(191389 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(191389 KB)

南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (03) : 179-180. DOI: 10.3969/j.jssn.1000-2006.2010.03.039

研究简报

松材线虫携带的荧光假单胞菌Pseudomonas fluorescens GcM5-1A菌株分泌的毒素及其对黑松的毒性

¹.青岛大学生物系，山东青岛²⁶⁶0⁷¹；².南京林业大学森林资源与环境学院，江苏南京²¹00³⁷

作者信息 +

Toxins secreted by Pseudomonas fluorescens GcM5-1A carried by pine wood nematode and their toxicities to Japanese black pine

LI Shengnan¹, GUO Daosen¹, ZHAO Boguang², LI Ronggui¹

Author information +

文章历史 +

摘要

从松材线虫携带的致病细菌荧光假单胞菌Pseudomonas fluorescens GcM5-1A菌株的培养液中分离、纯化得到一种50 ku的蛋白质，其N-末端氨基酸顺序为ALSVNTNITS，证明其为荧光假单胞标准菌株P.fluorescens Pf-5的鞭毛蛋白相似，同时该蛋白能与Pf-5菌株鞭毛蛋白抗体反应，证明其为相同的鞭毛蛋白。两个环二肽同时从GcM5-1菌株培养液中被分离和鉴定，它们是cyclo(-Pro-Val-)和cyclo(-Pro-Tyr-)。生测表明此次分离鉴定的鞭毛蛋白和两个环二肽对黑松愈伤组织及幼苗均具毒性。采用细胞免疫荧光分析、细胞染色观察、电导率测定、基因组DNA电泳分析技术对上述鉴定的鞭毛蛋白与黑松愈伤组织的作用及致死方式进行了研究，结果表明：鞭毛蛋白与黑松细胞之间存在直接的互作；鞭毛蛋白处理的细胞其细胞膜皱缩变形、细胞质浓缩、细胞核变形，形成若干小核，细胞质中的RNA降解；处理细胞着色力增强，细胞培养液的电导率增加，说明鞭毛蛋白科增加细胞膜的透性；基因组DNA电泳分析证实处理细胞的DNA发生断裂，但无明显的梯子形成，推测鞭毛蛋白对黑松细胞的致死方式为非典型的细胞凋亡。

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

.青岛大学生物系，山东青岛0；.南京林业大学森林资源与环境学院，江苏南京00. 松材线虫携带的荧光假单胞菌Pseudomonas fluorescens GcM5-1A菌株分泌的毒素及其对黑松的毒性[J]. 南京林业大学学报（自然科学版）. 2010, 34(03): 179-180 https://doi.org/10.3969/j.jssn.1000-2006.2010.03.039

LI Shengnan, GUO Daosen, ZHAO Boguang, LI Ronggui. Toxins secreted by Pseudomonas fluorescens GcM5-1A carried by pine wood nematode and their toxicities to Japanese black pine[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2010, 34(03): 179-180 https://doi.org/10.3969/j.jssn.1000-2006.2010.03.039

中图分类号： S763

参考文献

[1]Zhao B G, Liang B, Zhao L G. Influence of pine wood nematode on production of phytotoxins of an accompanying pathogenic bacterial strain[J]. Journal of Beijing Forestry University, 2005, 27: 71-75.
[2]Zhao B G, Wang H L, Han S F, et al. Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China[J]. Nematology, 2003, 5: 899-906.
[3]Schmitz F J, Vanderah D J, Hollenbeak K H. Metabolites from the marine sponge Ted aniaignis[J]. Journal of Organic Chemistry, 1983, 8: 39-41.
[4]Mota M M, Braasch H B, Penas A C, et al. First report of Bursaphelenchus xylophilus in Portugal and in Europe[J]. Nematology, 1999, 1: 727-734.
[5]Guo D S, Cong P J, Li L, et al. Determination of bacterial number carried by a pine wood nematodes and culture of sterilized nematodes on calli of Pinus thunbergii[J]. Journal of Qingdao University, 2002, 15: 29-31.
[6]Kawazu K, Kaneko N. Asepsis of the pine wood nematode isolateOKD-3 causes it to lose its pathogenicity[J]. Japanese Journal of Nematology, 1997, 27: 76-80.
[7]Oku H, Shiraishi T, Kurozumi S. Pine wilt toxin, the metabolite of a bacterium associated with a nematode[J]. Naturwissenschaften, 1980, 67: 198-199.
[8]Zhao B G, Li R G. Pine Wilt Disease[M]. New York: Springer Publishing Company, 2008.
[9]Guo Q Q, Guo D S, Zhao B G, et al. Two cyclic dipeptides from Pseudomonas fluorescens GcM5-1A carried by the pine wood nematode and their toxicities to Japanese black pine suspension cells and seedlings in vitro[J]. Journal of Nematology, 2007, 39(3): 243-247.
[10]Guo D S, Zhao B G, Li R G, et al. Purification of flagellin of Pseudomonas fluorescens GcM5-1A carried by the pine wood nematote, Bursaphelenchus xylophilus, and its in vitro toxicity to a suspension of cells of Pinus thunbergii[J]. Russian Journal of Nematology, 2008, 16(2): 151-157.
[11]Li S N, Guo D S, Zhao B G, et al. Lethal effect of flagellin secreted by Pseudomonas fluorescens on cells of Pinus thunbergii[J]. Acta Botanica BorealiOccidentalia Sinica, 2008, 28(11): 2154-2158.
[12]Ormerod M G, O’nell C F, Robertson D, et al . Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA[J]. Exp Cell Res, 1994, 211(2): 231-237.
[13]Catchpole D, Bernard W S. Etoposideinduced cytotoxicity in two human Tcell Leukemic lines :delayed membrane permeability rather than DNA fragmentation as an indicator of programmed cell death[J] . Cancer Res, 1993, 53(18): 4287-4296.
[14]Gross H, Stockwell V O, Henkels M D, et al. The genomisotopic approach: a systematic method to isolate products of orphan biosynthetic gene clusters[J]. Chemistry & Biology, 2007, 14: 53-63.
[15]Li X, Lin H, Zhang W, et al. Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102: 12990-12995.