南京林业大学学报(自然科学版) ›› 2012, Vol. 36 ›› Issue (05): 47-50.doi: 10.3969/j.jssn.1000-2006.2012.05.008

• 研究论文 • 上一篇    下一篇

青钱柳幼胚愈伤组织的诱导

张金凤,方升佐*,尚旭岚,徐锡增   

  1. 南京林业大学森林资源与环境学院,江苏南京210037
  • 出版日期:2012-09-30 发布日期:2012-09-30
  • 基金资助:
    收稿日期:2011-11-17修回日期:2012-04-25
    基金项目:江苏高校优势科技创新团队科研计划(苏教科[2009]10号);国家林业局林业科学技术推广项目﹙[2011]18号);国家林业公益性行业科研专项项目(200904046)
    第一作者:张金凤,硕士生。*通信作者:方升佐,教授。E-mail: fangsz@njfu.edu.cn。
    引文格式:张金凤,方升佐,尚旭岚,等. 青钱柳幼胚愈伤组织的诱导[J]. 南京林业大学学报:自然科学版,2012,36(5):47-50.

Callus induction from young embryos of Cyclocarya paliurus

ZHANG Jinfeng,FANG Shengzuo*,SHANG Xulan,XU Xizeng   

  1. College of Forest Resources and Environment, Nanjing Forestry University,Nanjing 210037,China
  • Online:2012-09-30 Published:2012-09-30

摘要: 以青钱柳幼胚为外植体,研究了不同幼胚期、基本培养基种类、植物生长调节剂种类及其浓度配比对愈伤组织诱导的影响。结果表明,不同发育期的幼胚诱导出的愈伤组织在形态上差异较大,青钱柳种子发育至子叶胚中期较适合愈伤组织的诱导。青钱柳幼胚愈伤诱导时基本培养基以改良DKW效果最佳,诱导率达到了88.89 %,且无褐化现象;含2,4-D与KT组合的培养基较适宜愈伤组织的诱导,其中2,4-D(0.5 mg/L)+KT (2.0 mg/L)和2,4-D (1.0 mg/L)+KT (1.0 mg/L)的组合对青钱柳幼胚诱导愈伤组织效果最佳,诱导率分别为80.00 %和78.33 %。

Abstract: Using young embryos of Cyclocarya paliurus as explants, we investigated the effects of embryo development period, basic media, types and the concentrations of plant growth regulators on callus induction of C. paliurus. The results showed that there were obvious differences in the shape of callus among different embryo development periods, and the middle cotyledon embryos were most suitable for callus induction. As a basic media, improved DKW medium was the best selection for young embryo callus induction, of which induction rate could hit to 88.89 % with no browning phenomenon. The medium containing 2,4-D and KT was more appropriate for callus induction. The 2,4-D (0.5 mg/L)+KT (2.0 mg/L) as well as 2,4-D (1.0 mg/L)+KT (1.0 mg/L) were optimal for callus induction of C. paliurus young embryo, and the induction rates were 80.00 % and 78.33 % respectively.

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