南京林业大学学报(自然科学版) ›› 2001, Vol. 25 ›› Issue (06): 80-82.doi: 10.3969/j.jssn.1000-2006.2001.06.021

• 研究论文 • 上一篇    下一篇

银杏、板栗不同组织DNA的提取

沈永宝;施季森   

  1. 国家林业局南方林木种子检验中心;江苏南京210037;国家林业局南方林木种子检验中心;江苏南京210037
  • 出版日期:2001-12-18 发布日期:2001-12-18

DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut

SHEN Yong-bao.SHI Ji-sen   

  1. Southern Forest Tree Seed Center of National Forestry Bureau, Nanjing 210037,China
  • Online:2001-12-18 Published:2001-12-18

摘要: <正>利用CTAB方法提取银杏、板栗的冬芽、嫩叶、半木质化韧皮部、老枝韧皮部和成熟叶子的 DNA。结果表明,除成熟叶子外,其它 4种组织的 DNA 较容易提取,且 DNA的纯度较高,A260/A280值都在 1.8以上,每 100 mg材料的产量为 22~50Gμg。清晰明亮的 PCR产物也表明所提取的DNA可满足DNA分子标记的要求。

Abstract: This paper shows that the genomic DNA from different tissues of Ginkgo and Chinese chest- nut was extracted by CTAB method. The results indicate that except for mature leaf, other tissues, uch as dormant bud, young leaf, phloem and half-lignous phloem, can be used to extract DNA easily and successfully. In addition,the value of A260/A280 for each DNA samples above mentioned is over 1. 8,and the quantity is over 22~50 g per 100 mg. Also,clear and bright products of RAPD reveal that the DNA may meet needs of DNA molecular markers.

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