丛枝菌根真菌对盐胁迫下桢楠光合生理的影响

doi:10.12302/j.issn.1000-2006.202005007

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南京林业大学学报（自然科学版） ›› 2021, Vol. 45 ›› Issue (1): 101-106.doi: 10.12302/j.issn.1000-2006.202005007

丛枝菌根真菌对盐胁迫下桢楠光合生理的影响

崔令军(), 刘瑜霞, 林健, 石开明^*()

生物资源保护与利用湖北省重点实验室,湖北民族大学,湖北恩施 445000

收稿日期:2020-05-06 接受日期:2020-11-09 出版日期:2021-01-30 发布日期:2021-02-01
通讯作者: 石开明
基金资助:
生物资源保护与利用湖北省重点实验室开放基金项目(PT012007);湖北省教育厅项目(B2017094);湖北省“双一流”建设专项资金项目;湖北民族大学博士启动基金项目(MY2017B029)

Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress

CUI Lingjun(), LIU Yuxia, LIN Jian, SHI Kaiming^*()

Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi 445000, China

Received:2020-05-06 Accepted:2020-11-09 Online:2021-01-30 Published:2021-02-01
Contact: SHI Kaiming

摘要/Abstract

摘要：

【目的】研究盐胁迫条件下,桢楠接种丛枝菌根真菌(AMF)后叶片光合色素含量、气体交换参数和叶绿素荧光参数的变化情况,以期为桢楠在盐碱地造林提供参考。【方法】采用分光光度法测定叶绿素a(Chl a)、叶绿素b(Chl b)和类胡萝卜素(Car)含量,采用Li-6400便携式光合仪测定气体交换参数和叶绿素荧光参数。【结果】不同浓度NaCl胁迫下,AMF接种与未接种处理桢楠光合色素含量均呈现显著差异,AMF显著提高了桢楠叶片的光合色素含量。盐胁迫下AMF接种处理后桢楠叶片的净光合速率(P_n)、气孔导度(G_s)、胞间CO₂浓度(C_i)和蒸腾速率(T_r)显著高于未接种处理;AMF接种桢楠叶片的最大光化学效率(F_v/F_m)、实际光化学效率(Φ_PSⅡ)、光化学淬灭系数(qP)变化不显著,而未接种处理则显著降低;未接种处理的非光化学淬灭系数(NPQ)在盐浓度为300 mmol/L时显著升高,而AMF接种处理的NPQ没有显著变化。双因素方差分析表明,接种AMF对Chl a、Chl b、Car含量和Φ_PSⅡ有显著影响。【结论】AMF通过改善光合色素含量、气体交换参数和叶绿素荧光参数,提高了桢楠对光能的利用能力,从而减轻了盐分对桢楠造成的伤害,表明AMF能够增强桢楠的耐盐性,进而促进桢楠在盐碱性土壤中生长。

关键词: 桢楠, 丛枝菌根真菌, 盐胁迫, 光合特性

Abstract:

【Objective】 The effects of arbuscular mycorrhizal fungi (AMF) and salt stress on the photosynthetic pigment content, gas exchange parameters and chlorophyll fluorescence parameters of Phoebe zhennan were studied to provide a reference for afforestation using P. zhennan in saline-alkali land. 【Method】 The contents of chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Car) were determined by spectrophotometry, and the gas exchange parameters and chlorophyll fluorescence parameters were determined using a Li-6400 portable photosynthesis instrument. 【Result】Under the different concentrations of salt stress, the contents of Chl a, Chl b and Car in the leaves of P. zhennan were significantly different between the AMF and non-AMF treatments, and the AMF significantly increased the chlorophyll content. The net photosynthetic rate (P_n), stomatal conductance (G_s), intercellular CO₂ concentration (C_i) and transpiration rate (T_r) in the leaves of P. zhennan with the AMF treatment were significantly higher than those of the non-AMF treatment. The maximal photochemical efficiency of photosystem Ⅱ (F_v/F_m), PSⅡ quantum yield (Φ_PSⅡ), and photochemical quenching coefficient (qP) of the AMF treatment leaves under salt stress did not change significantly, whereas those in the non-AMF treatment leaves decreased significantly. The non-photochemical quenching coefficient (NPQ) of non-AMF treatment leaves increased significantly at 300 mmol/L salt concentration, while the AMF treatment leaves were not significantly changed. The two-way analysis of variance showed that the AMF had significant effects on the contents of Chl a, Chl b, Car and Φ_PSⅡ. 【Conclusion】The AMF improves the utilization of light energy by P. zhennan by increasing the photosynthetic pigment content, gas exchange parameters, and chlorophyll fluorescence parameters, thereby reducing salt damage to P. zhennan. This shows that the AMF can enhance the salt tolerance of P. zhennan and promote its growth in saline-alkaline soils.

Key words: Phoebe zhennan, arbuscular mycorrhizal fungi (AMF), salt stress, photosynthetic characteristics

中图分类号:

S718

崔令军,刘瑜霞,林健,等. 丛枝菌根真菌对盐胁迫下桢楠光合生理的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 101-106.

CUI Lingjun, LIU Yuxia, LIN Jian, SHI Kaiming. Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(1): 101-106.DOI: 10.12302/j.issn.1000-2006.202005007.

图/表 4

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参考文献 27

[1]	潘晶, 黄翠华, 罗君, 等. 盐胁迫对植物的影响及AMF提高植物耐盐性的机制[J]. 地球科学进展, 2018,33(4):361-372.
	PAN J, HUANG C H, LUO J, et al. Effects of salt stress on plant and the mechanism of arbuscular mycorrhizal fungi enhancing salt tolerance of plants[J]. Adv Earth Sci, 2018,33(4):361-372.DOI: 10.11867/j.issn.1001-8166.2018.04.0361.
[2]	张恒, 刘晓婷, 陈嵩, 等. 盐胁迫下三倍体小黑杨杂种无性系叶片蛋白质差异表达分析[J]. 南京林业大学学报(自然科学版), 2020,44(2):59-66.
	ZHANG H, LIU X T, CHEN S, et al. Analysis of differentially expressed proteins in leaves of triploid Populus simonii × P. nigra hybrid clones under salt stress[J]. J Nanjing For Univ (Nat Sci Ed), 2020,44(2):59-66.DOI: 10.3969/j.issn.1000-2006.201904027.
[3]	肖斌, 汪媛艳. 盐胁迫对罗布麻和大花白麻种子萌发的影响[J]. 草业科学, 2020,37(2):314-319.
	XIAO B, WANG Y Y. Effects of salt stress on seed germination of Apocynum venetum and Poacynum hendersonii[J]. Pratacultural Sci, 2020,37(2):314-319.DOI: 10.11829/j.issn.1001-0629.2019-0212.
[4]	贺忠群, 李焕秀, 汤浩茹, 等. 丛枝菌根真菌对NaCl胁迫下番茄内源激素的影响[J]. 核农学报, 2010,24(5):1099-1104.
	HE Z Q, LI H X, TANG H R, et al. Effect of arbuscular mycorrhizal fungi on tomato endogenous under nacl stress[J]. J Nucl Agric Sci, 2010,24(5):1099-1104.
[5]	姜超强, 李杰, 刘兆普, 等. 盐胁迫对转AtNHX1基因杨树光合特性与叶绿体超微结构的影响[J]. 西北植物学报, 2010,30(2):301-308.
	JIANG C Q, LI J, LIU Z P, et al. Photosynthetic characteristics and chloroplast ultrastructure of transgenic poplar under NaCl stress[J]. Acta Bot Boreali-Occidentalia Sin, 2010,30(2):301-308.
[6]	闫文华, 吴德军, 燕丽萍, 等. 盐胁迫下白蜡无性系苗期的耐盐性综合评价[J]. 北京林业大学学报, 2019,41(11):44-53.
	YAN W H, WU D J, YAN L P, et al. Comprehensive evaluation of salt tolerance of clones of Fraxinusin spp. seedling stage under salt stress[J]. J Beijing For Univ, 2019,41(11):44-53.DOI: 10.13332/j.1000-1522.20180438.
[7]	宋福强, 杨国亭, 孟繁荣, 等. 丛枝菌根(AM)真菌对大青杨苗木根系的影响[J]. 南京林业大学学报(自然科学版), 2005,29(6):35-39.
	SONG F Q, YANG G T, MENG F R, et al. The effects of arbuscular mycorrhizal fungi on the radicular system of Populus ussuriensis seedlings[J]. J Nanjing For Univ (Nat Sci Ed), 2005,29(6):35-39.DOI: 10.3969/j.issn.1000-2006.2005.06.009.
[8]	RUIZ-LOZANO J M, PORCEL R, AZCÓN C, et al. Regulation by arbuscular mycorrhizae of the integrated physiological response to salinity in plants:new challenges in physiological and molecular studies[J]. J Exp Bot, 2012,63(11):4033-4044.DOI: 10.1093/jxb/ers126. doi: 10.1093/jxb/ers126
[9]	黄小辉, 陈道静, 冯大兰. 不同基质条件下丛枝菌根真菌对桑树生长的影响[J]. 南京林业大学学报(自然科学版), 2019,43(3):9-16.
	HUANG X H, CHEN D J, FENG D L. The effects of arbuscular mycorrhiza fungi on the growth of mulberry in different nursery substrates[J]. J Nanjing For Univ (Nat Sci Ed), 2019,43(3):9-16.DOI: 10.3969/j.issn.1000-2006.201810005.
[10]	COURTY P E, BUÉE M, OIEDHIOU A G, et al. The role of ectomycorrhizal communities in forest ecosystem processes:new perspectives and emerging concepts[J]. Soil Biology & Biochemistry, 2010,42(5):679-698.DOI: 10.1016/j.soilbio.2009.12.006. doi: 10.1016/j.soilbio.2009.12.006
[11]	张美月, 陶秀娟, 樊建民, 等. 磷和丛枝菌根真菌对盐胁迫草莓光合作用的影响[J]. 河北农业大学学报, 2009,32(4):71-75.
	ZHANG M Y, TAO X J, FAN J M, et al. Effect of phosphorus stress and AMF on photosynjournal in strawberry under salt stress[J]. J Agric Univ Hebei, 2009,32(4):71-75.
[12]	ZHANG Z F, ZHANG J C, HUANG Y Q. Effects of arbuscular mycorrhizal fungi on the drought tolerance of Cyclobalanopsis glauca seedlings under greenhouse conditions[J]. New For, 2014,45(4):545-556.DOI: 10.1007/s11056-014-9417-9. doi: 10.1007/s11056-014-9417-9
[13]	黄志, 许炜萍, 郁昉斌, 等. 接种AMF对弱光环境及盐胁迫下甜瓜光合特性的影响[J]. 西北植物学报, 2018,38(2):307-315.
	HUANG Z, XU W P, YU F B, et al. Photosynjournal responses of Cucumis melo seedlings to Glomus under low light and salt stress[J]. Acta Bot Boreali-Occidentalia Sin, 2018,38(2):307-315.DOI: 10.7606/j.issn.1000-4025.2018.02.0307.
[14]	DJITININGO DIATTA I L, KANE A, AGBANGBA C E, et al. Inoculation with arbuscular mycorrhizal fungi improves seedlings growth of two Sahelian date palm cultivars (Phoenix dactylifera L.,cv.Nakhla Hamra and cv.Tijib) under salinity stresses[J]. Adv Biosci Biotechnol, 2014,5(1):64-72.DOI: 10.4236/abb.2014.51010. doi: 10.4236/abb.2014.51010
[15]	KHALIL H A. Influence of vesicular-Arbuscula mycorrhizal fungi (Glomus spp.) on the response of grapevines rootstocks to salt stress[J]. Asian J Crop Sci, 2013,5(4):393-404.DOI: 10.3923/ajcs.2013.393.404. doi: 10.3923/ajcs.2013.393.404
[16]	梁倩倩, 王维华, 郭绍霞, 等. 盐胁迫下丛枝菌根对牡丹光合作用的影响[J]. 青岛农业大学学报(自然科学版), 2013,30(2):79-83.
	LIANG Q Q, WANG W H, GUO S X, et al. Effects of arbuscular mycorrhizae on photosynjournal of Paeonia suffruticosa grown under salt stress[J]. J Qingdao Agric Univ (Nat Sci Ed), 2013,30(2):79-83.DOI: 10.3969/J.ISSN.1674-148X.2013.02.001.
[17]	孙玉芳, 宋福强, 常伟, 等. 盐碱胁迫下AM真菌对沙枣苗木生长和生理的影响[J]. 林业科学, 2016,52(6):18-27. doi: 10.11707/j.1001-7488.20160603
	SUN Y F, SONG F Q, CHANG W, et al. Effect of arbuscular mycorrhizal fungi on growth and physiology of Elaeagnus angustifolia seedlings subjected to salinity stress[J]. Sci Silvae Sin, 2016,52(6):18-27.DOI: 10.11707/j.1001-7488.20160603.
[18]	唐明. 菌根真菌提高植物耐盐性[M]. 北京: 科学出版社, 2010.
	TANG M. Improvement on salt tolerance of plants by mycorrhizal fungi[M]. Beijing: Science Press, 2010.
[19]	GARCÍA I V, MENDOZA R E. Arbuscular mycorrhizal fungi and plant symbiosis in a saline-sodic soil[J]. Mycorrhiza, 2007,17(3):167-174.DOI: 10.1007/s00572-006-0088-z. doi: 10.1007/s00572-006-0088-z
[20]	郑鹏丽, 黄晓蓉, 费永俊, 等. 水分胁迫对桢楠幼树光合生理特性的影响[J]. 中南林业科技大学学报, 2019,39(10):64-70.
	ZHENG P L, HUANG X R, FEI Y J, et al. Effects of water stress on photosynthetic physiology characteristics of Phoebe zhennan seedlings[J]. J Central South Univ For Technol, 2019,39(10):64-70.DOI: 10.14067/j.cnki.1673-923x.2019.10.010.
[21]	崔令军, 刘瑜霞, 林健, 等. 盐胁迫下丛枝菌根真菌对桢楠根系生长和激素的影响[J]. 南京林业大学学报(自然科学版), 2020,44(4):119-124.
	CUI L J, LIU Y X, LIN J, et al. Effects of arbuscular mycorrhizal fungi on roots growth and endogenous hormones of Phoebe zhennan under salt stress[J]. J Nanjing For Univ (Nat Sci Ed), 2020,44(4):119-124.DOI: 10.3969/j.issn.1000-2006.201912030.
[22]	王彬, 胡红玲, 胡庭兴, 等. 干旱胁迫对桢楠幼树生长及光合特性的影响[J]. 西北农林科技大学学报(自然科学版), 2019,47(2):79-87,96.
	WANG B, HU H L, HU T X, et al. Effect of drought stress on photosynthetic characteristics and growth of Phoebe zhennan seedlings[J]. J Northwest A & F Univ (Nat Sci Ed), 2019,47(2):79-87,96.DOI: 10.13027/j.cnki.jnwafu.2019.02.010.
[23]	ASHRAF M A, AKBAR A, PARVEEN A, et al. Phenological application of selenium differentially improves growth,oxidative defense and ion homeostasis in maize under salinity stress[J]. Plant Physiol Biochem, 2018,123:268-280.DOI: 10.1016/j.plaphy.2017.12.023. doi: 10.1016/j.plaphy.2017.12.023 pmid: 29275208
[24]	GARCÉS-RUIZ M, CALONNE-SALMON M, PLOUZNIKOFF K, et al. Dynamics of short-term phosphorus uptake by intact mycorrhizal and non-mycorrhizal maize plants grown in a circulatory semi-hydroponic cultivation system[J]. Front Plant Sci, 2017,8:1471.DOI: 10.3389/fpls.2017.01471. doi: 10.3389/fpls.2017.01471 pmid: 28890723
[25]	ZHU X Q, TANG M, ZHANG H Q. Arbuscular mycorrhizal fungi enhanced the growth,photosynjournal,and calorific value of black locust under salt stress[J]. Photosynthetica, 2017,55(2):378-385.DOI: 10.1007/s11099-017-0662-y. doi: 10.1007/s11099-017-0662-y
[26]	PORCEL R, AROCA R, AZCON R, et al. Regulation of cation transporter genes by the arbuscular mycorrhizal symbiosis in rice plants subjected to salinity suggests improved salt tolerance due to reduced Na⁺ root-to-shoot distribution[J]. Mycorrhiza, 2016,26(7):673-684.DOI: 10.1007/s00572-016-0704-5. doi: 10.1007/s00572-016-0704-5 pmid: 27113587
[27]	PORCEL R, REDONDO-GÓMEZ S, MATEOS-NARANJO E, et al. Arbuscular mycorrhizal symbiosis ameliorates the optimum quantum yield of photosystem II and reduces non-photochemical quenching in rice plants subjected to salt stress[J]. J Plant Physiol, 2015,185:75-83.DOI: 10.1016/j.jplph.2015.07.006. doi: 10.1016/j.jplph.2015.07.006 pmid: 26291919

NaCl浓度/ (mmol·L^-1) NaCl concentration	叶绿素a含量/(mg·g^-1) Chl a content		叶绿素b含量/(mg·g^-1) Chl b content		叶绿素a/b Chl a/Chl b		类胡萝卜素含量/(mg·g^-1) Car content
NaCl浓度/ (mmol·L^-1) NaCl concentration	+AMF	-AMF	+AMF	-AMF	+AMF	-AMF	+AMF	-AMF
0	2.894±0.265 a	2.534±0.264 a	1.187±0.245 a	1.082±0.213 a	2.438±0.248 a	2.342±0.185 a	1.668±0.126 a	1.517±0.157 a
100	2.945±0.358 b	2.456±0.382 b	1.194±0.211 b	1.014±0.128 b	2.422±0.339 b	2.384±0.206 b	1.704±0.178 b	1.463±0.186 b
200	2.687±0.412 b	2.262±0.164 bc	1.175±0.143 a	0.919±0.126 c	2.287±0.247 c	2.461±0.182 a	1.571±0.181 b	1.422±0.177 b
300	2.445±0.218 b	1.883±0.177 c	0.981±0.127 b	0.873±0.112 c	2.492±0.324 a	2.157±0.231 c	1.314±0.176 c	1.264±0.163 c

处理 treatment	叶绿素a Chl a	叶绿素b Chl b	叶绿素 a/b Chl a/ Chl b	类胡萝卜素 Car	P_n	G_s	C_i	T_r	F_v/F_m	Φ_PSⅡ	光化学淬灭系数 qP	非光化学淬灭系数 NPQ
AMF	**	*	*	*	NS	NS	NS	NS	NS	*	NS	NS
NaCl	NS	NS	*	NS	**	*	*	**	NS	**	*	NS
AMF×NaCl	NS	NS	NS	*	*	**	*	*	*	NS	NS	NS

丛枝菌根真菌对盐胁迫下桢楠光合生理的影响

Effects of AMF on photosynthetic characteristics of Phoebe zhennan under salt stress

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