伴矿景天WRKY基因家族鉴定及镉胁迫响应分析

doi:10.12302/j.issn.1000-2006.202201015

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南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2): 49-60.doi: 10.12302/j.issn.1000-2006.202201015

伴矿景天WRKY基因家族鉴定及镉胁迫响应分析

王剑超¹^,²(), 邱文敏²(), 金康鸣², 陆铸畴², 韩小娇², 卓仁英², 刘晓光³^,^*(), 何正权¹^,^*()

1.三峡区域植物遗传与种质创新重点实验室(三峡大学),三峡大学生物与制药学院,湖北宜昌 443002
2.中国林业科学研究院亚热带林业研究所,浙江省林木育种技术研究重点实验室,浙江杭州 311400
3.唐山市农业科学研究院,河北唐山 063001

收稿日期:2022-01-12 修回日期:2022-06-25 出版日期:2023-03-30 发布日期:2023-03-28
通讯作者: * 何正权(zhq_he@163.com),教授,负责选题与实验方案设计;刘晓光(nyslxg@126.com),副研究员,负责数据分析与论文修改。
基金资助:
国家重点研发计划子课题(2016YFD080080104)

Comprehensive analysis of WRKY gene family in Sedum plumbizincicola responding to cadmium stress

WANG Jianchao¹^,²(), QIU Wenmin²(), JIN Kangming², LU Zhuchou², HAN Xiaojiao², ZHUO Renying², LIU Xiaoguang³^,^*(), HE Zhengquan¹^,^*()

1. Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement (CTGU),Biotechnology Research Center,China Three Gorges University, Yichang 443002, China
2. Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
3. Tangshan Academy of Agriculture Science, Tangshan 063001, China

Received:2022-01-12 Revised:2022-06-25 Online:2023-03-30 Published:2023-03-28

摘要/Abstract

摘要：

【目的】WRKY 转录因子在植物响应非生物胁迫过程中发挥着重要的调控作用,目前超积累植物伴矿景天(Sedum plumbizincicola) WRKY转录因子的研究较少。进行SpWRKY基因家族的全基因组鉴定及其在镉胁迫下的表达模式分析可为后续SpWRKYs 基因克隆和耐镉功能分析提供参考。【方法】对伴矿景天WRKY基因家族进行全基因组鉴定和生物信息学分析,利用转录组数据和qRT-PCR 技术检测WRKY基因在镉胁迫下的表达模式并进行转基因拟南芥耐镉性的分析。【结果】伴矿景天基因组中共鉴定出77个SpWRKYs,非均匀地分布在各条染色体上;系统进化树分析发现,伴矿景天WRKY成员被分成3大类群(Ⅰ—Ⅲ),第Ⅱ类群又被分成5个亚群(Ⅱa—e);共线性分析发现,伴矿景天WRKY基因家族和拟南芥(Arabidopsis thaliana) 之间存在7个共线基因对,和玉吊钟(Kalanchoe fedtschenkoi)之间存在53个共线基因对;SpWRKYs基因之间存在 19 个片段复制基因对;启动子序列分析发现,SpWRKYs 启动子有多种与激素和逆境响应等相关的顺式调控元件;镉胁迫表达分析发现,7 个SpWRKYs 受镉胁迫显著上调表达,整体表达趋势呈现先增后减的状态;在拟南芥中过表达SpWRKY69发现,SpWRKY69可以增强Cd离子向地上部的转运速率,对植株镉耐性起到负调控作用。【结论】伴矿景天WRKY基因家族与其他物种的WRKY家族结构相似,片段复制是其主要进化动力之一。镉胁迫条件下部分成员相对表达量显著变化,而SpWRKY69能增加Cd离子向地上部的转运速率,故其他WRKY成员可能也参与调控植株镉耐性。

关键词: 伴矿景天, WRKY转录因子, 镉胁迫, 基因表达, 拟南芥转基因

Abstract:

【Objective】 WRKY transcription factors play important regulatory roles for a plant abiotic stress response. However, little information is available about WRKY transcription factors in the hyperaccumulating plant Sedum plumbizincicola. The identification of SpWRKY gene family members and analysis of their expression patterns under cadmium stress can provide a reference for molecular cloning of SpWRKYs gene and functional characterization of cadmium tolerance.【Method】 In this study, the genome-wide identification and bioinformatics analysis of WRKY gene family members were carried out. Expression patterns of WRKY genes under cadmium stress were derived from transcriptome data and qPCR, and the roles of SpWRKY69 in cadmium tolerance were assessed by the heterologous expression in Arabidopsis thaliana.【Result】 There were 77 SpWRKYs identified in S. plumbizincicola, unevenly distributed on the chromosomes. Based on the phylogenetic analysis, the SpWRKY proteins were classified into three groups (Ⅰ-Ⅲ), among which the second group was further divided into five subgroups (Ⅱa-e). The synteny analysis showed that there were seven collinear gene pairs in the S. plumbizincicola and A. thaliana WRKY gene family and 53 collinear gene pairs for Kalanchoe fedtschenkoi; 19 pairs of SpWRKYs were identified as the segmental duplication. Cis-regulatory elements related to stress and hormone responses were found in the promoters of SpWRKYs. The expression profiles showed that the expression levels of the seven SpWRKYs were significantly up-regulated under cadmium stress, exhibiting a pattern of increasing initially and then decreasing. Over-expression of SpWRKY69 in A. thaliana showed that SpWRKY69 can enhance the transport rate of Cd ions to the shoot and play a negative role in regulating plant cadmium tolerance.【Conclusion】 Structural features of WRKY gene family members in S. plumbizincicola are similar to those of other species, and the fragment duplication is one of the main evolutionary forces. The relative expression levels of some members change significantly under cadmium stress, and SpWRKY69 can increase the transport rate of Cd ions to the shoot, and so other WRKY members may also be involved in the regulation of plant cadmium tolerance. These results provide a foundation for further functional characterization of SpWRKYs related to Cd tolerance.

Key words: Sedum plumbizincicola, WRKY transcription factor, cadmium stress, gene expression, Arabidopsis transformation

中图分类号:

S718

王剑超,邱文敏,金康鸣,等. 伴矿景天WRKY基因家族鉴定及镉胁迫响应分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 49-60.

WANG Jianchao, QIU Wenmin, JIN Kangming, LU Zhuchou, HAN Xiaojiao, ZHUO Renying, LIU Xiaoguang, HE Zhengquan. Comprehensive analysis of WRKY gene family in Sedum plumbizincicola responding to cadmium stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(2): 49-60.DOI: 10.12302/j.issn.1000-2006.202201015.

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基因名 gene ID	序列 sequence	用途 application
SpWRKY42-RT-F	GTTGCCGCAAATGTTTAGCC	伴矿景天筛选基因 qRT-PCR引物与内参引物
SpWRKY42-RT-R	ATAGCTGCTGTGAAATGCGG
SpWRKY72-RT-F	AGGGTTCTTGTTCGGACTGA
SpWRKY72-RT-R	AGCGCTTCTCTGAACCTTCT
SpWRKY40-RT-F	AGTGTAGCTGCTGAATCCGA
SpWRKY40-RT-R	AAAAGTCCAACTCCGGCCTA
SpWRKY46-RT-F	GACAACCGGCACTTTTGACT
SpWRKY46-RT-R	TAGGCTGTGTTGGAGTTGGT
SpWRKY39-RT-F	AGTTTACCGGCGAAGATGGA
SpWRKY39-RT-R	TCCAAATCCGACGACTCTGT
SpWRKY69-RT-F	CCAAACCATAAAGCCACCGA
SpWRKY69-RT-R	TCGCCGGAACTCTTACAACT
SpWRKY29-RT-F	TCACGGCGGAGATATCGAAA
SpWRKY29-RT-R	AAGAGCATCGATACGTCCGT
UBC9-F	TGGCGTCGAAAAGGATTCTGA
UBC9-R	CCTTCGGTGGCTTGAATGGATA
SpWRKY69-F	ATGGCCGTCGACCTCGT	SpWRKY69全长基因克隆引物与拟南芥内参引物
SpWRKY69-R	TCACGACGACTCTAGAATGAGT
actin-F	GCACCCTGTTCTTCTTACCG
actin-R	AACCCTCGTAGATTGGCACA

伴矿景天WRKY基因家族鉴定及镉胁迫响应分析

Comprehensive analysis of WRKY gene family in Sedum plumbizincicola responding to cadmium stress

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