The cytoplasmic vitrification and freezing temperature of Chinese fir and masson pine seeds were measured by using differential scanning calorimetry (DSC). And the relationship between formation of glassy state and lipid as well as sugar content was analyzed. The results showed as follows: (1) The glassy state was detected in the seeds of Chinese fir. Its cytoplasmic vitrification temperature (Tg) is--52.386=C. The freezing temperature of Chinese fir seeds is-34. 323 C. The glassy state was not detected in the seeds of masson pine. The freezing temperature of masson pine is--24. 826℃. (2) The gross lipid content in Chinese fir seeds is lower than that in masson pine seeds. The ratio of Oligosaccharide/Disaccharide(O/D value) in Chinese fir seeds is higher than that in masson pine seeds. These indicated that the lower lipid content and higher ()ligosaccharide content were beneficial for the formation of glassy state. (3) The moisture content is not correlated with the formation of glassy state.