Effects of 7 strains of ectomycorrhizal fungi on germination and growth of Pinus massoniana, P. thunbergii and P. elliottii seedlings were studied in this paper. The results showed that inoculating ectomycorrhizal fungi when the seeds were planted increased percentage of germination and made seeds germinate ahead of time. Ectomycorrhizal fungi can evidently promote the growth of pine seedlings in the height, root collar diameter, number of lateral root and dry weight. Among three sorts of pine seedlings, P. massoniana grew the best, followed by P. thunbergii, then P. elliottii. B. edulis had the best effect on promoting growth, followed A. pantherina; Pt2 had better effect on promoting growth than P. massoniana and P. thunbergii, but Pt1 and L. aemthystea had weaker effects on promoting growth; B. speciosus and R. luteous only had evident effects on promoting growth if it is compared with P. massoniana.