Dormancy breaking techniques and ultrastructural changes were studied in seeds of Sorbus amabilis Cheng ex Yü. Seeds were under different treatments including various levels of GA3, NaOH, H2SO4, cold stratification and mechanical scarification. The results showed that 90 ％ of the seeds of S. amabilis germinated by mechanical scarification and cold stratification for a period of 75—90 days, which are effective methods to break dormancy. GA3+cold stratification can also break seed dormancy, but less effective than treated with directly cold stratification.Other treatments could not break seed dormancy. Microstructural characters of seed surface and cotyledon cells were observed under scanning and transmission electron microscope in different period of cold stratification, the results showed that germination rate and dormancy breaking effects are directly related to the degree of cuticles broken and disintegration on seed surfaces, and directly related to structural changes and deposition degrade in cotyledon cells.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1]刘登义,沈浩,杨月红,等.黄山花楸种群遗传多样性研究［J］.应用生态学报, 2003,14(12):2141-2144.
[2]庄凤君,王继丰,王臣.国产及引进花楸属植物研究与应用进展［J］.国土与自然资源研究,2008(1):94-96.
[3]沈海龙,杨玲,张建瑛,等.花楸树种子休眠影响因素与萌发特性研究［J］.林业科学,2006,42(10):133-139.
[4]杨万霞,泭香香,方升佐.青钱柳种子的种皮构造及其对透水性的影响［J］.南京林业大学学报：自然科学版,2005,29 (5):25-28.
[5]何志,唐宇丹,石雷,等.猬实种子休眠特性研究［J］.园艺学报,2008,35 (10):1505-1510.
[6]Nadjafia F, Bannayan M, Tabrizia L, et al. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium［J］. Journal of Arid Environments, 2006(64):542-547.
[7]Sharifi M, Pouresmael M. Breaking seed dormancy in Bunium persicum by stratification and chemical substances［J］. Asian Journal of Plant Sciences, 2006,5(4):695-699.
[8]孙杰,熊军波,刘永志,等.野牛草种子休眠原因分析［J］.草地学报,2009,17(5):665-669.
[9]滕范例,赵辉.花楸种子不同处理方法发芽率的比较［J］.中国林副特产,2006(4):30-31.
[10]Daniel B P, Timothy E P. A comparison of the seed dormancy characteristics of Spartina patens and Spartina alterniflora (Poaceae) ［J］. American Journal of Botany, 1996, 83(1):11-14.
[11]Basharuddin J, Smith M L. Afterripening requirement, dormancy and germination in rowan (Sorbus aucuparia L.) ［C］//Edwards D G W. Dormancy and Barriers to Germination Proceedings of an International Symposium of IUFRO Project Group. Canada:Forestry Canada Pacific Forestry Centre,1993.
[12]董厚德,宫莉君.结缕草种子(颖果) 解除休眠前后的显微结构［J］.草地学报,2001,9(4):244-247.
[13]徐亮,李建东,殷萍萍,等.野生大豆种皮形态结构和萌发特性的研究［J］.大豆科学,2009,28(4):641-646.
PDF(4689231 KB)
