Abstract: Two kinds of detoxification methods, vacuum evaporation and complex extraction were applied to remove inhibitors presented in the dilute acid pretreated corn stover prehydrolyzate. The results showed that furfural was completely removed, while 5hydroxymethylfurfural was not removed at all by only vacuum evaporation. When the prehydrolyzate was condensed to 5.80 times, the concentration of acetic acid in the condensed prehydrolyzate and its removal ratio were 4.06 g/L and 60.45 %, respectively. The optimal conditions of complex extraction detoxification were that 30 % trialkylamine +50 % noctanol +20 % kerosene was at the ratio of organic to aqueous phase of 2〖DK〗∶1, and 25 ℃ for 60 min. At this time, the concentration of acetic acid was 1.43 g/L, and the removal ratio of acetic acid, furfural and 5hydroxymethylfurfural were 52.33 %, 100 % and 27.78 %, respectively.