JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (05): 51-55.doi: 10.3969/j.jssn.1000-2006.2012.05.009

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Effects of priming on cell cycle and germination of Pinus massoniana seeds

REN Shujuan, YU Fangyuan*, XU Liping   

  1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2012-09-30 Published:2012-09-30

Abstract: In order to reveal the cytological mechanism of promoting seed germination through seed priming, the differences of DNA content in embryonic cell of Pinus massoniana seeds after different priming treatments were studied by using flow cytometry, and seed germination percentage was also determined. The results showed that: (1) DNA replication in embryonic cell could be enhanced by PEG 8000 solution pretreatment, the ratio of tetraploid cells increased, the best treatment was a regime treated for 3-4 days using 20 % PEG 8000 solution. (2) The best priming treatment for increasing cell proliferation index was a regime treated in 20 % PEG 8000 solution for 3-4 days at 15 ℃. (3) Higher PEG 8000 concentration and longer pretreatment time were benefit for increasing germination percentage. (4) In a certain extent, there was a significant positive correlation between the cell proliferation index and seed germination percentage.

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