Effects of priming on cell cycle and germination of Pinus massoniana seeds

REN Shujuan, YU Fangyuan*, XU Liping

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (05) : 51-55.

PDF(793197 KB)
PDF(793197 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2012, Vol. 36 ›› Issue (05) : 51-55. DOI: 10.3969/j.jssn.1000-2006.2012.05.009

Effects of priming on cell cycle and germination of Pinus massoniana seeds

  • REN Shujuan, YU Fangyuan*, XU Liping
Author information +
History +

Abstract

In order to reveal the cytological mechanism of promoting seed germination through seed priming, the differences of DNA content in embryonic cell of Pinus massoniana seeds after different priming treatments were studied by using flow cytometry, and seed germination percentage was also determined. The results showed that: (1) DNA replication in embryonic cell could be enhanced by PEG 8000 solution pretreatment, the ratio of tetraploid cells increased, the best treatment was a regime treated for 3-4 days using 20 % PEG 8000 solution. (2) The best priming treatment for increasing cell proliferation index was a regime treated in 20 % PEG 8000 solution for 3-4 days at 15 ℃. (3) Higher PEG 8000 concentration and longer pretreatment time were benefit for increasing germination percentage. (4) In a certain extent, there was a significant positive correlation between the cell proliferation index and seed germination percentage.

Cite this article

Download Citations
REN Shujuan, YU Fangyuan*, XU Liping. Effects of priming on cell cycle and germination of Pinus massoniana seeds[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2012, 36(05): 51-55 https://doi.org/10.3969/j.jssn.1000-2006.2012.05.009

References

[1]郑光华. 种子生理研究[M]. 北京: 科学出版社,2004.
[2]焦旭雯, 赵树进. 流式细胞术在高等植物研究中的应用[J]. 热带亚热带植物学报, 2006,14(4): 354-358.
[3]Sliwinska E. Nuclear DNA replication and seed quality [J]. Seed Science Research, 2009,19(1): 15-25.
[4]国家质量技术监督局. GB 2772—1999 林木种子检验规程[S]. 北京:中国标准出版社,2000.
[5]吴后男.流式细胞术原理与应用教程[M].北京:北京大学医学出版社,2008.
[6]翟中和,王喜忠,丁明孝.细胞生物学[M]. 3版.北京:高等教育出版社,2007.
[7]Bino R J, de Vries J N, Kraak H L, et al. Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination [J]. Annals of Botany,1992,69(3): 231-236.
[8]Lanteri S, Saracco F, Kraak H L, et al. The effects of priming on nuclear replication activity and germination of pepper (Capsicum annuum) and tomato (Lycopersicon esculentum) seeds[J]. Seed Science Research,1994,4(2):81-87.
[9]Saracco F, Bino R J, Bergervoe J H V, et al. Influence of priminginduced nuclear replication activity on storability of pepper (Capsicum annuum L.) seeds [J]. Seed Science Research,1995,5(1): 25-29.
[10]Van Pijlen J G, Groot S P C, Kraak H L,et al. Effects of prestorage hydration treatments on germination performance, moisture content, DNA synthesis and controlled deterioration tolerance of tomato (Lycopersicon esculentum Mill.) seeds [J]. Seed Science Research,1996,6(2): 57-63.
[11]Gurusinghe S H, Cheng Z, Bradford K J. Cell cycle activity during seed priming is not essential for germination advancement in tomato [J]. Journal of Experimental Botany,1999,50(330): 101-106.
[12]De Castro R D, van Lammeren A A M, Groot S P C, et al. Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not [J]. Plant Physiology,2000,122(2): 327-335.
[13]Sliwinska E, Jendrzejczak E. Sugarbeet seed quality and DNA synthesis in the embryo in relation to hydrationdehydration cycles [J]. Seed Science and Technology,2002,30(3): 597-608.
[14]Sliwinska E, Sadowski H. Comparison of the effects of bioactive compounds and hydration treatments on germination and cell cycle activity of sugarbeet seeds [J]. Seed Science and Technology,2004,32(3):847-850.
[15]Kozeko L E, Troyan V M. The relationship between the mitotic activity and moisture content of recalcitrant seeds of Acer saccharinum(L.) during maturation, postmaturation drying and germination[J]. Seed Science Research,2000,10(3): 225-232.
[16]Dahal P, Bradford K J, Jones R A. Effect of priming and endosperm integrity on seed germination rates of tomato genotypes. I: Germination at suboptimal temperature [J]. Journal of Experimental Botany, 1990, 41(11):1441-1453.
[17]Taylor A G, Motes J E, Kirkham M B. Germination and seedling growth characteristic of three tomato species affected by water deficits[J]. Journal of the America Society for Horticultural Science, 1982, 107(1):282-285.
[18]De R, Kar R K. Seed germination and seedling growth of mungbean (Vigna radiata) under water stress induced by PEG6000 [J]. Seed Science & Technology, 1995, 23(2):301-308.
[19]Chamberlin M A, Horner H T, Palmer R G. Nuclear size and DNA content of the embryo and endosperm during their initial stages of development in Glycine max (Fabaceae)[J]. American Journal of Botany, 1993, 80(10): 1209-1215.
[20]Wyman J, Tremblay M F, Laliberte S. Cell cycle activation during imbibition and visible germination in embryos and megagametophytes of Jack pine (Pinus banksiana Lamb.)[J]. Annals of Botany, 1996, 78(2): 245-253.
PDF(793197 KB)

Accesses

Citation

Detail

Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

/