Abstract: With the middle and lower reaches of Yangtze River as survey region, under the support of observations from normalized differential vegetation index(NDVI)during years from 1998 to 2008 and benchmark weather stations from 1951 to 2011,using regional climate model RegCM3 and remote sensing data from MODIS, the relationship between vegetation condition and climate change had been analyzed, in perspectives of “point”, “vegetation coverage” and “area”, “vegetation cover type” respectively. The results showed that:(1)NDVI value and air temperature had the trend of ascending, precipitation mainly remained unchanged. From the point of annual time scale the relationship between NDVI and temperature appeared stronger positive correlation, and showed a weak negative correlation with precipitation.(2)In the selected three underlying surfaces, the trend of increasing temperature in staggered area of hills and urban and rural in Yangtze River delta region was the weakest, whose majority vegetation cover was farmland. In spring, summer and fall season, the trend looks like, forest area of Zhejiang, Jiangxi, Anhui mountain > mixed forest area in low hills of Anhui > staggered area of hills and urban and rural in Yangtze River delta region. Conditions in winter were the opposite. For the precipitation analysis, in the season of spring, summer and fall, the value in farm land areas increased significantly, and forest precipitation decreased significantly; in winter, all values in three areas increased.