JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2014, Vol. 38 ›› Issue (增刊): 17-19.doi: 10.3969/j.issn.1000-2006.2014.S1.004

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The genome size determination of Cerasus serrulata

LI Zhen, YI Xiangui, GU Yu, WANG Xianrong*   

  1. College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:1900-01-01 Published:1900-01-01

Abstract: The present study optimized the process of flow cytometry(FCM)for Cerasus with the purpose of determining the genome size of Cerasus serrulata and obtaining optimal buffer for its FCM process. The results showed that the LB01 was the best buffer for C. serrulata among the six analyzed buffers. This paper used Clycine max(L.)Merrill was the internal standard to test the genome size of C. serrulata, which was 366.67 Mb with C value 0.375 pg. That present results would lay foundations for ploidy identification of Cerasus serrulata.

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