Abstract: In order to explore the eco-hydrological process of Quercus acutissima forest in the hilly area of southern Jiangsu province and its influencing factors, rainfall distribution process of Q. acutissima forest was studied by using fixed-point observation method, from April to September 2012. The results showed that: during the study period, the cumulative precipitation was 470.7 mm and average precipitation was 10.0 mm and the small rainfall occupied most of the rainfall events, whose event frequency was 70.21% of the whole. There were significant positive correlation among throughfall, stemflow and canopy with rainfall. According to the regression models,the canopy storage capacity and the stem storage capacity of Q. acutissima were 0.87 mm and 0.11 mm respectively. The critical minimum rainfall for the occurrence of stemflow was 3.42 mm rainfall, rainfall intensity and air humidity had remarkable negative correlation with canopy interception rate. Monthly canopy interception rate increasing with the increase of monthly mean LAI under light rain.There was a significant linear relationship between leaf area index and the monthly mean rate of canopy interception. The average rate of canopy interception reached 20.48%.