JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (04): 105-110.doi: 10.3969/j.issn.1000-2006.2015.04.018

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Influence of different extraction methods on the chemical composition of grape pomace condensed tannins

LAN Ping1, HUANG Runzhou1*, ZHOU Dingguo1, BROSSE Nicolas2, CHRUSCIEL Laurent2   

  1. 1.College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
    2.Laboratoired’Etude et de Recherche sur le Materiau Bois, Faculte des Sciences et Techniques, Universite de Lorraine, Nancy 54500, France
  • Online:2015-07-31 Published:2015-07-31

Abstract: Condensed tannins were extracted from grape pomace by acid precipitation and lyophilisation, respectively. Influence of the recovery methods on the chemical structure and composition of tannin extracts was investigated by using Carbon Nuclear Magnetic Resonance(13C-NMR)and Matrix-Assisted Laser Desorption/Ionization Time of Flight(MALDI-TOF)Mass Spectrometry. The results showed that the procyanidin units played a dominait role in two kinds of condensed tannin extracts, and small proportion glucose and gallic acid substitutions bonded in procyanidin units which was linked together by C4-C8 bond. The polymerization degree of acidified condensed tannins was higher than that of lyophilized condensed tannins. In addition, relatively low carbonhydrate and high catechinc acid content in acidified condensed tannins were observed compared with lyophilized condensed tannins. Different extraction methods had great influence on the chemical composition of grape pomace tannin extracts.

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