Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation

doi:10.3969/j.issn.1000-2006.2015.04.023

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2015, Vol. 39 ›› Issue (04): 132-136.doi: 10.3969/j.issn.1000-2006.2015.04.023

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Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation

REN Jiwei¹, ZHOU Jie², XU Qianqian², LI Xin², ZHENG Zhaojuan², OUYANG Jia^2*

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

Online:2015-07-31 Published:2015-07-31

Abstract

Abstract: Bagasse sulfite pulp is a kind of pretreated cellulosic material, which can be easily hydrolyzed, and high glucose and total sugar yield can be obtained after enzymatic hydrolysis. In this study, the preparation of ethanol from bagasse sulfite pulp(BSP)was investigated using Pichia stipitis by simultaneous saccharification and fermentation(SSF). Effects of temperature, pH, inoculum size, substrate mass concentration and cellulase dosage on ethanol production were investigated. The results confirmed that ethanol could be produced from BSP using P. stipitis by SSF, and the optimal conditions were as follows: 36 ℃, pH 4.8, 0.13 g/L of inoculum size, 75 g/L of substrate mass concentration, 15 μmol/(min·g)cellulose of cellulase dosage. Under the optimal conditions, 17.89 g/L of ethanol mass concentration were produced in 72 h with 55.99% of theoretical yield. The optimized process of this study could provide technical references for ethanol production from BSP by SSF.

CLC Number:

TS713

REN Jiwei, ZHOU Jie, XU Qianqian, LI Xin, ZHENG Zhaojuan, OUYANG Jia. Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2015, 39(04): 132-136.

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Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation

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