Effects of plant growth regulator on cuttings rooting of Altingia gracilipessl

ZHANG Dandan,FAN Junjun,ZHAO Mingming,CAI Xiyan,ZHANG Wangxiang1,2,*

Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (01) : 41-47.

PDF(1496430 KB)
南京林业大学学报（自然科学版）
PDF(1496430 KB)
Journal of Nanjing Forestry University (Natural Sciences Edition） ›› 2018, Vol. 42 ›› Issue (01) : 41-47. DOI: 10.3969/j.issn.1000-2006.201709057

Effects of plant growth regulator on cuttings rooting of Altingia gracilipessl

  • ZHANG Dandan1,FAN Junjun1,ZHAO Mingming1,2,CAI Xiyan3,ZHANG Wangxiang1,2,*
Author information +
History +

Abstract

【Objective】Determine the suitable formula and understand the mechanism of rooting Altingia gracilipes cuttings to improve cutting survival rate and establish a cutting propagation system.【Method】In this study, a total of 13 treatment groups using four different concentrations(150,300,500 and 800 mg/L)of IAA, NAA or GGR and one water-treated control(CK)were used to treat 3-year-old Altingia gracilipes softwood seedlings. The rooting process, rooting(rooting rate, average root number, average root length)and the dynamic changes in nutrient content(soluble protein, soluble starch, soluble sugar)and oxidase activity(peroxidase(POD),polyphenol oxidase(PPO),indoleacetic acid oxidase(IAAO))were observed during the rooting process of Altingia gracilipes cuttings.【Result】①Plant growth regulators had a significant influence on rooting time and rooting of Altingia gracilipes cuttings. ②Rooting of cuttings in the treatment with 300 mg/L NAA for 2 h were the earliest to exhibit the rooting process, 12 d ahead of CK, and the rooting rate(78%),average root number(16.8),and average root length(4.27 cm),were, 3.4, 6.2 and 4.6 times that of CK. ③Dynamic changes in nutritive material content and oxidase activity in the process of Altingia gracilipes rooting from the adventitious root induction period to the early adventitious root formation,than the adventitious root formation stage, indicated that the peak or valley value of each indicator appeared at the same time as the beginning time of the early adventitious root or adventitious root formation stage, which reflected the synergy between the rooting process and physiological metabolism. ④The 300 mg/L NAA treatment significantly improved oxidase activities, the accumulation of soluble proteins.【Conclusion】Plant growth regulators sped up the process of synthesis and metabolism of nutrients and rooting, and improved the ability to regulate endogenous oxidase.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
ZHANG Dandan,FAN Junjun,ZHAO Mingming,CAI Xiyan,ZHANG Wangxiang1,2,*. Effects of plant growth regulator on cuttings rooting of Altingia gracilipessl[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2018, 42(01): 41-47 https://doi.org/10.3969/j.issn.1000-2006.201709057

References

[1] 张宏达.中国植物志:35卷[M].北京:科学出版社,1979:66-67.
[2] 胡晓静.乡土速生树种细柄阿丁枫栽培特性及造林模式的研究[D].福州:福建农林大学,2004. HU X J.Study on the cultivate characteristic and afforestation modle of Altingia gracilipessl[D].Fuzhou:Fujian Agriculture and Forestry University, 2004.
[3] 吴文谱.中国的细柄阿丁枫林[J].植物科学学报,1990,8(1):59-63. WU W P.The Altingia gracilipes forest in China [J].Journal of Wuhan Botanical Research, 1990, 8(1):59-63.
[4] 陈存及,陈伙法.阔叶树种栽培[M].北京:中国林业出版社, 2000:12-19.
[5] 胡裕玉.细柄阿丁枫造林技术及开发利用探讨[J].现代农业科技,2005(19):6-7. DOI:10.3969/j.issn.1007-5739.2005.19.003.
[6] 林小娟.细柄阿丁枫大田采种育苗初探[J].中国林副特产,2009(6):51-52.DOI:10.3969/j.issn.1001-6902.2009.06.023.
[7] 许晓红,史亚萍.细柄蕈树全光照扦插繁育技术[J].江西林业科技,2006(4):15-16. DOI:10.16259/j.cnki.36-1342/s.2006.04.006. XU X H, SHI Y P. Altingia gracilipes' cutting breeding technique of full exposure[J].Jiangxi Forestry Science and Technology,2006(4):15-16.
[8] 范俊俊,张往祥,蔡喜彦,等.细柄蕈树嫩枝扦插生根过程中内源激素动态特性研究[J].江苏林业科技,2014:41(4):11-14. DOI:10.3969/j.issn.1001-7380.2014.04.003. FAN J J, ZHANG W X, CAI X Y, et al. The endogenous hormones' variation during the adventitious root formation of Altingia gracilipes cuttings [J].Journal of Jiangsu Forestry Science & Technology, 2014, 41(4):11-14.
[9] 王良桂,杜旭华,王顺财,等.不同楸树品种(类型)嫩枝扦插生根能力及扦插繁殖技术[J].南京林业大学学报(自然科学版),2008, 32(5):127-130. DOI:10.3969/j.issn.1000-2006.2008.05.028. WANG L G, DU X H, WANG S C, et al. Study on the rooting ability of different varieties(types)Catalpa spp. and its un lignified branch cutting technique [J].Journal of Nanjing Forestry University(Natural Sciences Edition), 2008, 32(5):127-130.
[10] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[11] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1):248-254.DOI: 10.1016/0003-2697(76)90527-3.
[12] LAURENTIN A, EDWARDS C A. A microtiter modification of the anthrone-sulfuric acid colorimetric assay for glucose-based carbohydrates[J]. Analytical Biochemistry, 2003, 315(1):143-145.DOI: 10.1016/S0003-2697(02)00704-2.
[13] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2002.
[14] 郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004.
[15] NAIJA S, ELLOUMI N, JBIR N, et al. Anatomical and biochemical changes during adventitious rooting of apple rootstocks MM 106 cultured in vitro[J]. Comptes Rendus Biologies, 2008, 331(7): 518-525. DOI:10.1016/j.crvi.2008.04.002.
[16] 敖红,王昆,陈一菱,等.长白落叶松插穆内的营养物质及其对扦插生根的影响[J].植物研究,2002,22(3):301-304. DOI:10.3969/j.issn.1673-5102.2002.03.013. AO H, WANG K, CHEN Y L,et al. Nutritive material in cuttings of Larix olgensis and its effect on rooting [J].Bulletin of Botanical Research, 2002,22(3):301-304.
[17] 吕明霞.梅花硬枝扦插繁殖与贮藏养分的关系[J].浙江农业科学, 2000(4):200-202. DOI:10.3969/j.issn.0528-9017.2000.04.018. LV M X. Relations between propagation by hardwood cuttings of mume and nutrient reserves [J]. Journal of Zhejiang Agricultural Sciences, 2000,(4):200-202.
[18] 郭素娟,凌宏勤,李凤兰.白皮松插穗生根的生理生化基础研究[J].北京林业大学学报, 2004,26(2):43-47. DOI:10.3321/j.issn:1000-1522.2004.02.009. GUO S J, LING H Q, LI F L.Physiological and biochemical basis of rooting of Pinus bungeana cuttings[J]. Journal of Beijing Forestry University, 2004, 26(2):43-47.
[19] BASAK U C, DAS A B, DAS P. Metabolic changes during rooting in stem cuttings of five mangrove species[J]. Plant Growth Regulation, 1995, 17(2):141-148. DOI:10.1007/BF00024174.
[20] GOLDSMITH M H M. The transport of auxin[J]. Plant Biology, 1968, 1(19):347-360. DOI: 10.1146/annurev.pp.19.060168.002023..
[21] ROUT G R, SAMANTARAY S, ROUT M C, et al. Metabolic changes during rooting in stem cuttings of Casuarina equisetifolia L.: effects of auxin, the sex and the type of cutting on rooting [J].Plant Growth Regulation, 1996, 19(1):35-43.
[22] 武维华.植物生理学[M].北京:科学技术出版社, 2003,213-239.
[23] 詹亚光,杨传平,金贞福,等.白桦插穗生根的内源激素和营养物质[J].东北林业大学学报,2001,29(4):1-4. DOI:10.3969/j.issn.1000-5382.2001.04.001. YAN Y G,YANG C P,JIN Z F,et al. Endogenous hormones and nutritive material in softwood cuttings of Betula platyphylla during rooting[J]. Journal of Northeast Forestry University, 2001, 29(4):1-4.
[24] 董胜君,刘明国,戴菲,等.山杏嫩枝扦插繁殖试验[J].经济林研究, 2013,31(1):176-180. DOI:10.14067/j.cnki.1003-8981.2013.01.028. DONG S J, LIU M G, DAI F, et al. Experiment of green-wood cutting propagation in Armeniaca sibirica[J]. Nonwood Forest Research, 2013, 31(1):176-180.
[25] 曾炳山,黄永芳,杨懋勋,等.柚木嫩枝扦插生根过程中营养物质的研究[J].中南林业科技大学学报, 2013,33(2):1-4. DOI:10.14067/j.cnki.1673-923x.2013.02.009. ZENG B S, HUANG Y F, YANG M X, et al. Study on nutrient contents of Tectona grandis Linn. shoot in the course of soft-wood cutting propagation[J].Journal of Central South University of Forestry & Technology, 2013,33(2):1-4.
[26] 扈红军,曹帮华,尹伟伦,等.不同处理对欧榛硬枝扦插生根的影响及生根过程中相关氧化酶活性的变化[J].林业科学, 2007,43(12):70-75. DOI:10.3321/j.issn:1001-7488.2007.12.012. HU H J,CAO B H,YIN W L,et al. Effects of different treatments on hardwood-cutting rooting and related oxidase activity changes during rooting of Corylus avellana [J]. Scientia Silvae Sinicae, 2007, 43(12):70-75.
[27] LEWIS N G, YAMAMOTO E. Lignin: occurrence, biogenesis and biodegradation[J]. Annual Review of Plant Physiology & Plant Molecular Biology, 1990, 41: 455-496. DOI:10.1146/annurev.pp.41.060190.002323.
[28] JACKSON P A P, GALINHA C I R, PEREIRA C S,et al. Rapid deposition of extensin during the elicitation of grapevine callus cultures is specifically catalyzed by a 40-Kilodalton peroxidase [J].Plant Physiology, 2001,127(3):1065-1076. DOI:10.1104/pp.127.3.1065.
[29] ROUT G R. Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis(L.)Kuntze and associated biochemical changes [J].Plant Growth Regulation, 2006, 48(2):111-117. DOI: 10.1007/s10725-005-5665-1.
[30] PASSARDI F, COSIO C, PENEL C,et al. Peroxidases have more functions than a Swiss army knife [J]. Plant Cell Reports, 2005, 24(5):255-265. DOI: 10.1007/s00299-005-0972-6.
[31] NAG S, SAHA K, CHOUDHURI M A. Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting[J]. Journal of Plant Growth Regulation, 2001, 20(2):182-194. DOI: 10.1007/s003440010016.
[32] SYROS T, YUPSANIS T, ZAFIRIADIS H, et al. Activity and isoforms of peroxidases, lignin and anatomy, during adventitious rooting in cuttings of Ebenus cretica L [J]. Journal of Plant Physiology, 2004, 161(1):69-77.DOI:10.1078/0176-1617-00938
[33] YILMAZ H, TASKIN T, OTLUDIL B. Polyphenol oxidase activity during rooting in cuttings of grape(Vitis vinifera L.)varieties[J]. Turkish Journal of Botany, 2003, 27(6):495-498.
[34] BALAKRISHNAMURTHY G, RAO VNM. Changes in Phnols during Rhizogenesis in Rose(Rosa Bourboniana Desp.)[J].Current Science, 1988, 57(17):960-962.
[35] KIELISZEWSKA-ROKICHA B. Effect of treating scots pine(Pinus sylvestris L.)seedlings with phytohorm one on the growth of the root system and on the peroxidase and IAA oxidase enzyme activities in roots[J].Arboretum-Kornckie, 1989,32:207-219.
[36 ]WANG X L, ZHAO Z, QUAN J E.Indole-3-butyric acid on rooting and endogenous plant hormones in tetraploid and diploid Robinia pseudoacacia hardwood cuttings[J].International Journal of Experimental Botany,2011,80(1):93-100.
PDF(1496430 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉