A study on mechanism of propagation and shoot cutting of Monarda didyma

WEI Dongshan, ZHANG Qiuliang, CHANG Jinbao

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05) : 39-45.

PDF(2219929 KB)
南京林业大学学报（自然科学版）
PDF(2219929 KB)
JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2018, Vol. 42 ›› Issue (05) : 39-45. DOI: 10.3969/j.issn.1000-2006.201801045

A study on mechanism of propagation and shoot cutting of Monarda didyma

  • WEI Dongshan, ZHANG Qiuliang*, CHANG Jinbao
Author information +
History +

Abstract

【Objective】Identify the optimum multi-factor combination for propagation and the mechanism of rooting of shoot cutting propagation of Monarda didyma, with the goal of providing the theoretical basis and technical support for shoot cutting propagation of ground cover plants as applied in urban forests of cold and arid regions.【Method】Based on the orthogonal test of shoot cutting propagation, the optimized multi-factor combination for propagation was determined. Anatomical and physiological studies, as well as a morphological study of M. didyma were conducted to reveal the histologic origin of adventitious roots, rooting type, the basic law of nutrient metabolism, and the response of protective enzymes against the dynamic fluctuation of harmful substances.【Result】The optimal combination for shoot cutting propagation of M. didyma was A2B1C2D3(the substrate with the ratio of the vermiculite and peat soil is 7:3 and the cutting taken from the top with length 10 cm, ABT concentration to 1 000 mg/kg)with 100% root balls forming within 23 d. Different positions of cuttings on the branch substantially affected root ball forming rate. The root primordia originated by the induced pattern, and the mixed rooting mode produced the roots. The callus formations on the cutting base were independent from adventitious root production. There were significant and highly significant correlations between the dynamic variation contents of soluble protein, POD, SOD and MDA.【Conclusion】Shoot cuttings is suitable for the propagation of M. didyma. Crucial factors of shoot rooting were not totally dependent upon the existence of root primordium in the cutting slips, which should be a plant hereditary character and dependent upon use of key techniques. The response mechanism of soluble proteins and the plant enzyme protection system to the dynamic changes in harmful substances under stress played an important role in the formation of root primordium and the callus.

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
WEI Dongshan, ZHANG Qiuliang, CHANG Jinbao. A study on mechanism of propagation and shoot cutting of Monarda didyma[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(05): 39-45 https://doi.org/10.3969/j.issn.1000-2006.201801045

References

[1] 韦东山.地被植物多因素优化扩繁与栽培技术研究[D].呼和浩特:内蒙古农业大学,2016.
WEI D S.Combined and optimal techniques of propagation and cultivation of ground cover plants[D].Huhhot: Inner Mongolia Agricultural University,2016.
[2] BUBEl N. Herbs to grow in the shade[J].Country Journal,1991,8:69-70.
[3] SOVOVA H,SAJFRTOVA M,TOPIAR M. Supercritical CO2 extraction of volatile thymoquinone from Monarda didyma and M. fistulosa herbs[J].The Journal of Supercritical Fluids,2015,105(6):29-34.DOI:10.1016/j.supflu.2015.01.004.
[4] 孙宜.“柯歹粉”美国薄荷组培技术[J].中国花卉盆景,2002(9):21-22.
SUN Y.Tissue culture technique of Monarda‘Croftway Pink'[J].Chinese Flowers Miniascape,2002(9):21-22.
[5] 刘军,郝玉兰.美国薄荷的组织培养与植株再生[J].内蒙古科技与经济,2011,247(21):83-85.
LIU J,HAO Y L.Tissue culture and plant regeneration of Monarda didyma[J].Inner Mongolia Science Technology and Economy,2011,247(21):83-85.
[6] HAYASHI T,HEINS R D,CAMERON A C,et al.Ethephon influences flowering,height,and branching of several herbaceous perennials[J].Scientia Horticulturae,2001,91(14):305-234.DOI:10.1016/s0304-4238(01)00225-4.
[7] ITOH A,YAMAKURA T,KANZAKI M,et al.Rooting ability of cuttings relates to phylogeny,habitat preference and growth characteristics of tropical rainforest trees [J].Forest Ecology and Management,2002,168(1):275-287.DOI:10.1016/s0378-1127(01)00742-3.
[8] ALTINDAL E,BALTA F.Comparison of rooting capabilities of Turkish tea clones[J].Turkish Journal of Agriculture and Forestry,2002,26(4):195-202.
[9] 潘瑞炽.植物生理学[M].北京:高等教育出版社,2012.
[10] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[11] 邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[12] 许晓岗.垂丝海棠、揪子的扦插生根机理研究[D].南京:南京林业大学,2006.
XU X G.The researeh of rooting Mechanism of Malus halliana & Malus prunifolia cutting[D].Nanjing:Nanjing Forestry University,2006.
[13] 张宪政.植物生理学实验技术[M].沈阳:辽宁科学技术出版社,1994.
[14] 郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社.
[15] NAN J X,JIN Z T.Studies on the propagation of peach trees(Prunus persica)by cuttings [J].Horticulture Environment and Biotechnology,1985,26(4): 342-349.
[16] 韦东山,常金宝,胡柏芳,等.紫花鼠尾草多因素优化扩繁条件及生根机制研究[J].干旱区资源与环境,2017,31(11):154-160.DOI:10.13448/j.cnki.jalre.2017.363.
WEI D S,CHANG J B,HU B F,et al.Mechanism of propagation and rooting of Salvia farinacea under the optimized multi-factors of managerial actions [J].Journal of Arid Land Resources and Environment,2017,31(11):154-160.
[17] STRZELECKA K.Anatomical structure and adventitious root formation in Rhododendron ponticum L.cuttings[J].Acta Scientiarum Polonorum Hortorum Cultus,2007,6(2):15-22.
[18] 孟丙南.四倍体刺槐扦插技术优化及生根机理研究[D].北京:北京林业大学,2010.
MENG B N.Study on optimizing of cuttage propagation and mechanism of rooting in tetraploid Robinia pseudoacacia[D].Beijing:Beijing Forestry University,2010.
[19] 魏国汶,宋小民,黄冬华,等.金边瑞香的扦插生根研究[J].江西农业学报,1998,10(3):56-59.
WEI G W,SONG X M,HUANG D H,et al.Study on the cutting and rooting in Daphne odora Thunb var. marginata Max[J].Acta Agriculturae Jiangxi,1998,10(3):56-59.
[20] 张玉臣.白木香扦插繁殖技术及生根机理研究[D].北京:中国林业科学研究院,2010.
ZHANG Y C.Cutting propagation techniques and rooting machanism of Aquilaria sinensis(Lour.)Gilg[D].Beijing:China Academy of Forestry Sciences,2010.
[21] 袁利利.华北五角枫扦插繁殖技术及生根机理的研究[D].泰安:山东农业大学,2012.
YUAN L L.The studys on the propagation techniques and rooting machanism of setm cuttings Acer turncatum Bunge[D].Taian:Shandong Agricultural University,2012.
[22] 刘家栋,翟兴礼,王启明.蜡梅扦插繁殖技术研究[J].河南大学学报(自然科学版),2001,31(3):87-88.
LIU J D,ZHAI X L,WANG Q M.Study of wintersweet cuttage breeding technology[J].Journal of Henan University(Natural Science),2001,31(3):87-88.
[23] 王东光.闽楠嫩枝扦插繁殖技术及生根机理研究[D].北京:中国林业科学研究院,2013.
WANG D G.Soft cutting propagation techniques and rooting mechanism of Phoebe bournei(Hemsl.)Yang[D].Beijing:China Academy of Forestry Sciences,2013.
[24] 有祥亮.欧洲栓皮槭和银叶椴引种的适应性、繁殖技术经济价值研究[D].北京:北京林业大学,2006.
YOU X L.Study on adaptability of introduction,propagative technique and economic values of Acer campestre and Tilia tomentosa[D].Beijing:Beijing Forestry University,2006.
[25] 张猛.费约果引种适应性及扦插生理的研究[D].雅安:四川农业大学,2009.
ZHANG M.Study on adaptability of introduction and rooting mechanism of cuttings of Feigoa(Feigao selloiana Berg)[D].Ya'an:Sichuan Agricultural University,2009.
[26] HARTMANN H T.Effect of season of collecting,dolebutyric acid,and pre-planting storage treatment on rooting of Marianna plum,peach,and quince hardwood cuttings[J].American Society for Horticultural Science,1958(71):57-66.
[27] 杨青珍,王锋,李娟,等.榛子绿枝插条生根的解剖学观察[J].中国农学通报,2006,22(7):154-156.DOI: 10.3969/j.issn.1000-6850.2006.07.040.
YANG Q Z,WANG F,LI J,et al.The anatomical observation on rooting process of tender cuttings of Hazelnut[J]. Chinese Agricultural Science Bulletin,2006,22(7):154-156.
[28] 潘健,夏日红,程家寿,等.柃木插条生根的解剖学研究[J].植物资源与环境学报,2008,17(1):62-65.
PAN J,XIA R H,CHENG J S,et al.Anatomical study on rooting of Eurya japonica cutting [J].Journal of Plant Resources and Environment,2008,17(1):62-65.
[29] 李隆云,张雪,杨宪.灰毡毛忍冬扦插生根的解剖学特征研究[J].中国中药杂志,2010,35(4):431-434.DOI:10.4268/cjcmm20100407.
LI L Y,ZHANG X,YANG X.Anatom icalstudy on rooting of Lonicera macranthoides cutting[J].China Journal of Chinese Materia Medica,2010,35(4):431-434.
[30] 许晓岗,汤庚国,童丽丽.海棠果插穗扦插生根过程解剖学观察[J].南京林业大学学报(自然科学版),2006,30(7):77-80.DOI:10.3969/j.issn.1000-2006.2006.04.018.
XU X G,TANG G G,TONG L L.The anatomical observation on the rooting of the cutting of Malus prunifolia Borkh [J].Journal of Nanjing Forestry University(Natural Sciences Edition),2006,30(7):77-80.
[31] 冯承浩,姚辉,吴鸿.广藿香扦插繁殖不定根形成的解剖学研究[J].西北植物学报,2004,24(4):732-736.DOI:10.3321/j.issn:1000-4025.2004.04.031.
FENG C H,YAO H,WU H.Histological study of initiation and development in adventitious roots in cuttings of Pogostemon cablin[J].Acta Botanica Boreali-Occidentalia Sinica,2004,24(4):732-736.
[32] 刘勇,肖德兴,黄长干,等.板栗嫩枝扦插生根解剖学特征研究[J].园艺学报,1997,24(1):8-12.
LIU Y,XIAO D X,HUANG C G,et al.The anatomical feasture study on the rooting of the tender branch cuttings of Castanea mollissima Blume[J].Acta Horticulturae Sinica,1997,24(1):8-12.
[33] 孙婷婷,胡宝忠,陈玉霞,等.黄刺玫(Rosa xanthina Lindl.)嫩枝扦插生根过程的解剖学研究[J].东北农业大学学报,2008,39(7):53-56.DOI:10.3969/j.issn.1005-9369.2008.07.013.
SUN T X,HU B Z,CHENG Y X,et al.Anatomical study on the rooting of the green shoot cutting propagation of Rosa xanthina Lindl[J].Journal of Northeast Agricultural University,2008,39(7):53-56.
[34] 臧忠婧,吴瑕.草原樱桃扦插繁殖生根解剖学分析[J].北方园艺,2010(3):35-37.
ZANG Z J,WU X.The Anatomical observation on rooting of the cutting ground cherry[J].Northern Horticulture,2010(3):35-37.
[35] 扈顺.四合木繁殖特性及扦插生根解剖学研究[D].呼和浩特:内蒙古农业大学,2014.
HU S.Research on reproductive characteristics and anatomy of Tetraena mongolica Maxim.[D].Huhhot: Inner Mongolia Agricultural University,2014.
[36] 刘云强,杨建民,彭伟秀,等.两种椴树嫩枝扦插生根的解剖学研究[J].河北农业大学学报,2004,27(2):33-37.DOI: 10.3969/j.issn.1000-1573.2004.02.009.
LI Y Q,YANG J M,PENG W X,et al.The anatomical study on the rooting of the green shoot cutting propagation of two kinds of Tilia[J].Journal of Agricultural University of Hebei,2004,27(2):33-37.
[37] 张晓平,方炎明.杂种鹅掌楸插穗不定根发生与发育的解剖学观察[J].植物资源与环境学报,2003,12(1):10-15.DOI: 10.3969/j.issn.1674-7895.2003.01.003.
ZHANG X P,FANG Y M.Anatomical observation of the origin and development of adventitious roots in hybrid tuliptrees during cutting[J].Journal of Plant Resources & Environment,2003,12(1):10-15.
[38] 林艳,詹亚光,刘玉喜,等.白桦嫩枝扦插不定根形成的解剖观察[J].东北林业大学学报,1996,24(3):15-19.
LIN Y,ZHAN Y G,LIU Y X,et al.The anatomical study on formation of adventitious root of softwood cutting in Betula platyphytlla[J].Journal of Northeast Forestry University,1996,24(3):15-19.
[39] 马英,李明鹤,张新华,等.杉木嫩枝扦插不定根形成的解剖学研究[J].华中农业大学学报,1998,17(1):81-83.
MA Y,LI M H,ZHANG X H,et al.An anatomical study on the formation of adventitious roots of chinese fir cuttings[J]. Journal of Huazhong Agricultural,1998,17(1):81-83.
[40] 郭素娟,凌宏勤,潘万春,等.白皮松插穗的生根特性与其解剖构造的关系[J].北京林业大学学报,2004,26(5):43-46+103.DOI: 10.3321/j.issn:1000-1522.2004.05.010.
GUO S J,LING H Q,PAN W CH,et al.Relationships between rooting characteristics and anatomical structure of Pinus bungeana cuttings[J].Journal of Beijing Forestry University,2004,26(5):43-46+103.
[41] HEAMAN J C,OWENS J N.Callus formation and root initiation in stem cuttings of Douglas-fir(Pseudotsuga menziesii(Mirb.)Franco)[J].Canadian Journal of Forest Research,1972,2(2): 121-134.DOI:10.1139/x72-023.
PDF(2219929 KB)

Accesses

Citation

Detail
Sections
Recommended
The full text is translated into English by AI, aiming to facilitate reading and comprehension. The core content is subject to the explanation in Chinese.

Author

Reader

Reviewer

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

/

〈 〉