Isolation and identification of endophytic fungi from Cyclocarya paliurus (Batal.) Iljinskaja

doi:10.3969/j.issn.1000-2006.201812001

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2020, Vol. 44 ›› Issue (2): 26-34.doi: 10.3969/j.issn.1000-2006.201812001

Special Issue: 青钱柳叶有效成分专题

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Isolation and identification of endophytic fungi from Cyclocarya paliurus (Batal.) Iljinskaja

KOU Xiaolin¹(), XIE Nan¹, WU Caie¹^,²^,^*(), FAN Gongjian¹, FU Xiangxiang²

1. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2018-12-03 Revised:2019-11-19 Online:2020-03-30 Published:2020-04-01
Contact: WU Caie E-mail:391153013@qq.com;wucaie@njfu.edu.cn

Abstract

Abstract:

【Objective】This study aimed to identify the endophytic fungi produced flavonoids in Cyclocarya paliurus (Batal.) Iljinskaja and explore a new method to extract flavonoids. 【Method】The endophytic fungi in the branches, leaves, roots, and bark of C. paliurus (Batal.) Iljinskaja were isolated using the tissue separation method. By observing the morphological characteristics of the endophytes, the fermentation products of the endophytic fungi from C. paliurus (Batal.) Iljinskaja were identified using the chromogenic reaction method, thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). The endophytic fungi produced flavonoids were screened, and the total flavonoid production was determined. Moreover, the strains were identified based on the morphological characteristics and the phylogenetic analysis of the intervening sequences of the fungal rDNA. 【Result】In total, 67 strains of endophytic fungi were obtained; through the identification of morphological characteristics, they were identified to belong to three classes, four orders, six families and ten genera. Four endophytic fungi producing flavonoids were finally identified: PZ06, CY12, CP06 and PP03. The most abundant flavonoids, with a yield of 3.61 mg/L, were produced by strain PZ06. Molecular identification combined with morphological identification indicated that PZ06, CP06 and PP03 belong to the genusAlternaria, whereas CY12 belongs to the genus Eupenicillium. The dominant flavonoid-producing endophytic fungi of C. paliurus (Batal.) Iljinskaja belong to the genus Alternaria. 【Conclusion】 The study of endophytic fungi in C. paliurus (Batal.) Iljinskaja is a key significance for the development and utilization of endophytes. In addition to identifying the endophytic fungi, the present study also provides a novel method for the extraction of active substances fromC. paliurus (Batal.) Iljinskaja plants.

Key words: Cyclocarya paliurus(Batal.) Iljinskaja, flavonoids, endophytic fungi, ITS analysis, scanning electron microscope, isolation, identification

CLC Number:

S718.81

KOU Xiaolin, XIE Nan, WU Caie, FAN Gongjian, FU Xiangxiang. Isolation and identification of endophytic fungi from Cyclocarya paliurus (Batal.) Iljinskaja[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(2): 26-34.

Figures/Tables 7

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References 46

[1]	中国科学院中国植物志编辑委员会. 中国植物志 [M]. 北京: 科学出版社, 1994.
	Chinese Flora Editorial Committee of the Chinese Academy of Sciences. Flora of China [M]. Beijing: Science Press, 1994.
[2]	应俊生, 张玉龙. 中国种子植物特有属[M]. 北京: 科学出版社, 1994: 91-105.
	YING J S, ZHANG Y L. Endemic genera of Chinese seed plants [M]. Beijing: Science Press, 1994: 91-105.
[3]	李婷婷, 吴彩娥, 方升佐, 等. 青钱柳胶囊的装量稳定性及其降糖功效评价[J]. 食品工业科技, 2013,34(19):337-340.
	LI T T, WU C E, FANG S Z, et al. Content stability and hypoglycemic effect of Cyclocarya paliurus capsule [J]. Science and Technology of Food Industry, 2013,34(19):337-340. DOI: 10.13386/j.issn1002-0306.2013.19.034.
[4]	吴彩娥, 方升佐, 徐琳, 等. 青钱柳叶三萜大孔吸附树脂纯化工艺[J]. 农业机械学报, 2010,41(9):143-147.
	WU C E, FANG S Z, XU L, et al. Purification of triterpenes in Cyclocarya paliurus leaves by macroporous resins [J]. Transactions of the Chinese Society of Agricultural Machinery, 2010,41(9):143-147. DOI: CNKI: SUN: NYJX.0.2010-09-028.
[5]	PETRINI O. Fungal endophytes of tree leaves[M]. Brock/Springer Series in Contemporary Bioscience. New York: Springer, 1991, 179-197. DOI: 10.1007/978-1-4612-3168-4_9.
[6]	吕婉婉, 赵明, 龙光强, 等. 药用植物内生真菌活性物质研究进展[J]. 中华中医药学刊, 2018,36(3):654-658.
	LYU W W, ZHAO M, LONG G Q, et al. Research advance of endophytic fungi active substance in medicinal plants[J]. Chinese Archives of Traditional Chinese Medicine, 2018,36(3):654-658. DOI: 10.13193/j.issn.1673-7717.2018.03.033.
[7]	王梅霞, 陈双林, 霍娟. 一株银杏内生真菌的分离及其产黄酮类物质的初步研究[J]. 工业微生物, 2004,34(2):15-18.
	WANG M X, CHEN S L, HUO J. Isolation of a Ginkgo biloba endophytic fungus and preliminary study on its flavonoids [J]. Industrial Microbiology, 2004,34(2):15-18. DOI: 10.3969/j.issn.1001-6678.2004.02.004.
[8]	WILSON A D. Resources and testing of endophyte infected germplasm in national grass repository collections [M]// Systematics, Ecology, and Evolution of Endophytic Fungi in Grasses and Woody Plants. Paul Minnesota: APS Press, 1996.
[9]	STIERLE A, STROBEL G, STIERLE D. Taxol and taxane production by Taxomycetes andreanae, an endophytic fungus of Pacific Yew[J]. Science, 1993, 260(5105):214-216. DOI: 10.1126/science.8097061. doi: 10.1126/science.8097061
[10]	DENG Z J, CAO L X. Fungal endophytes and their interactions with plants in phytoremediation: a review[J]. Chemosphere, 2017, 168:1100-1106. DOI: 10.1016/j.chemosphere.2016.10.097. doi: 10.1016/j.chemosphere.2016.10.097
[11]	RHO H, HSIEH M, KANDEL S L, et al. Do endophytes promote growth of host plants under stress? a meta-analysis on plant stress mitigation by endophytes[J]. Microbial Ecology, 2017, 75(2):407-418. DOI: 10.1007/s00248-017-1054-3. doi: 10.1007/s00248-017-1054-3
[12]	卢东升, 卢帅, 潘中超, 等. 水杉内生真菌生物多样性与生态分布[J]. 南京林业大学学报(自然科学版), 2013,37(6):33-36.
	LU D S, LU S, PAN Z C, et al. Biological diversity and ecological distribution of endophytic fungi in Metasequoia glyptostroboides [J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2013,37(6):33-36. DOI: 10.3969/j.issn.1000-2006.2013.06.007.
[13]	中华人民共和国药典委员会. 中华人民共和国药典(2005年版)第一部[M]. 北京: 化学工业出版社, 2005.
	The Pharmacopoeia Commission of the People’s Republic of China. The first part of the pharmacopoeia of the People’s Republic of China (2005 Edition) [M]. Beijing: Chemical Industry Press, 2005.
[14]	HUANG W Y, CAI Y Z, HYDE K D, et al. Biodiversity of endophytic fungi associated with 29 traditional Chinese medicinal plants[J]. Fungal Diversity, 2008, 33:61-75.
[15]	赵庆云, 樊明涛, 师俊玲. 产黄酮银杏内生菌的分离与鉴定[J]. 西北农业学报, 2007,16(2):169-173.
	ZHAO Q Y, FAN M T, SHI J L. Isolation and identification of flavones-forming endophytes from Ginkgo biloba L .[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2007,16(2):169-173. DOI: 10.3969/j.issn.1004-1389.2007.02.040.
[16]	戴传超, 余伯阳, 赵玉婷, 等. 大戟科4种植物内生真菌分离与抑菌研究[J]. 南京林业大学学报(自然科学版), 2006,30(1):79-83.
	DAI C C, YU B Y, ZHAO Y T, et al. The screening and identification of endophytic fungi from four species of family Euphorbiaceae and the strains’ antibacterial activity[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2006,30(1):79-83. DOI: 10.3969/j.jssn.1000-2006.2006.01.020.
[17]	赵晓璐, 周宁, 谢庆武, 等. 产黄酮荷叶内生真菌的筛选与鉴定[J]. 中成药, 2016,38(11):2512-2515.
	ZHAO X L, ZHOU N, XIE Q W, et al. Screening and identification of endophytic fungi producing flavonoids in lotus leaves[J]. Chinese Traditional Patent Medicine, 2016,38(11):2512-2515. DOI: 10.3969/j.issn.1001-1528.2016.11.046.
[18]	徐慧超, 孙婷媛, 翟李欣, 等. 产甘草次酸内生真菌RE7的鉴定及抑菌活性研究[J]. 中国新药杂志, 2016,35(1):102-105.
	XU H C, SUN T Y, ZHAI L X, et al. Identification of glycyrrhetinic acid-producing endophytic fungus RE7 from Glycyrrhiza uralensis and its antibacterial activity [J]. Chinese Journal of New Drugs, 2016,35(1):102-105.
[19]	寇晓琳, 谢楠, 吴彩娥, 等. 青钱柳内生真菌多样性及其代谢产物的特性[J]. 福建农林大学学报(自然科学版), 2019,48(4):500-507.
	KOU X L, XIE N, WU C E, et al. Diversity of endophytic fungi from Cyclocarya paliurus and the characteristics of its metabolites [J]. Journal of Fujian Agriculture and Foresery University(Natural Science Edition), 2019,48(4):500-507. DOI: 10.13323/j.cnki.j.fafu(nat.sci.).2019.04.014.
[20]	张海龙, 李善春, 卢维浩, 等. 银杏内生真菌多样性与产黄酮类物质真菌的分离和鉴定[J]. 土壤, 2015,47(1):135-141.
	ZHANG H L, LI S C, LU W H, et al. Isolation and identification of endophytic fungi diversity and flavonoid-producing fungus from Ginkgo biloba [J]. Soils, 2015,47(1):135-141. DOI: 10.13758/j.cnki.tr.2015.01.021.
[21]	ZHENG N, YAO F H, LIANG X F, et al. A new phthalide from the endophytic fungus Xylaria sp. GDG-102[J]. Natural Product Research, 2018, 32(7):168-170. DOI: 10.1080/14786419.2017.1311892.
[22]	布芳芳, 余仲东, 侯姣姣, 等. 古侧柏鳞叶内生真菌分离及其抑菌活性研究[J]. 中国森林病虫, 2016,35(3):12-16.
	BU F F, YU Z D, HOU J J, et al. Isolation, identification and antifungal activity of endophytic fungi from ancient Platycladus orientalis [J]. Forest Pest and Disease, 2016,35(3):12-16. DOI: 10.3969/j.issn.1671-0886.2016.03.003.
[23]	李向楠, 周稚凡, 刘风路, 等. 云南榧种子内生真菌的分离鉴定及抑菌活性筛选[J]. 生物灾害科学, 2016,39(1):20-26.
	LI X N, ZHOU Z F, LIU F L, et al. Isolation and identification of endophytes from seeds of Torreya yunnanensis and its antimicrobial activity screening [J]. Biological Disaster Science, 2016,39(1):20-26. DOI: 10.3969/j.issn.2095-3704.2016.01.003.
[24]	PATIL R H, PATIL M P, MAHESHWARI V L. Chapter 5 - Bioactive secondary metabolites from endophytic fungi: a review of biotechnological production and their potential applications[J]. Studies in Natural Products Chemistry, 2016, 3:35-43. DOI: 10.1016/B978-0-444-63601-0.00005-3.
[25]	成军, 赵玉英, 崔育新, 等. 杜仲叶黄酮类化合物的研究[J]. 中国中药杂志, 2000,25(5):284-286.
	CHENG J, ZHAO Y Y, CUI Y X, et al. Studies on flavonoids from leave of Eucommia ulmoides Oliv [J]. China Journal of Chinese Materia Medica, 2000,25(5):284-286. DOI: 10.3321/j.issn:1001-5302.2000.05.010.
[26]	杨万霞, 佘诚棋, 方升佐. 青钱柳叶中黄酮类化合物的地理变异[J]. 浙江林学院学报, 2009,26(4):522-527.
	YANG W X, SHE C Q, FANG S Z. Geographic variation of flavonoid compounds inCyclocarya paliurus leaves [J]. Journal of Zhejiang Forestry College, 2009,26(4):522-527. DOI: 10.3969/j.issn.2095-0756.2009.04.012.
[27]	易醒, 黄志勇, 谢明勇, 等. 反相高效液相色谱法测定青钱柳中黄酮化合物含量[J]. 南昌大学学报, 2001,25(2):161-164.
	YI X, HUANG Z Y, XIE M Y, et al. Determination of flavonoids in the leaves of Cyclocarya paliurus(Batal.) Iijinsk by reversed-phase high-performance liquid chromatography [J]. Journal of Nanchang University (Natural Science), 2001,25(2):161-164. DOI: 10.3969/j.issn.1006-0464.2001.02.014.
[28]	GONZALEZ-TEUBER M, VILO C, BASCUNAN-GODOY L. Molecular characterization of endophytic fungi associated with the roots of Chenopodium quinoa in habiting the Atacama desert chile[J]. Genomics Data, 2017, 11:109-112. DOI: 10.1016/j.gdata.2016.12.015. doi: 10.1016/j.gdata.2016.12.015
[29]	李晓倩. 一种适合于PCR扩增的真菌基因组DNA提取方法[J]. 山东农业大学学报(自然科学版), 2011,42(1):49-53.
	LI X Q. Rapid extraction of fungal genomic DNA for PCR amplification[J]. Journal of Shandong Agricultural University (Natural Science Edition), 2011,42(1):49-53. DOI: 10.3969/j.issn.1000-2324.2011.01.009.
[30]	史素琴, 李北辰, 朱平. 人参病原真菌的扫描电子显微镜观察[J]. 特产研究, 1991,1:9-11.
	SHI S Q, LI B C, ZHU P. Scanning electron microscopic observation of ginseng pathogenic fungi[J]. Special Wild Economic Animal and Plant Research, 1991,1:9-11. DOI: CNKI:SUN:TCYA.0.1991-01-003.
[31]	何映霞, 冉雪琴, 王嘉福. 珙桐产黄酮内生真菌的分离和鉴定[J]. 贵州农业科学, 2008,36(3):3-6.
	HE Y X, RAN X Q, WANG J F. Isolation and identification of endophytic fungi strains producing flavonoids from Davidia involucrate Baill [J]. Guizhou Agricultural Sciences, 2008,36(3):3-6. DOI: 10.3969/j.issn.1001-3601.2008.03.002.
[32]	颜霞, 李希尧, 李伟国. 喜树内生真菌的分离及其代谢产物的初步研究[J]. 西北农业学报, 2008,17(3):315-318.
	YAN X, LI X Y, LI W G. Isolation of endophytes from Camptotheca acuminata decne and the pilot study of metabolites [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2008,17(3):315-318. DOI: 10.3969/j.issn.1004-1389.2008.03.069.
[33]	刘紫英. 产黄酮成分的绶草内生真菌的鉴定[J]. 菌物学报, 2011,30(1):133-137.
	LIU Z Y. Endophytic fungi producing flavonoids from Spiranthes sinensis [J]. Mycosystema, 2011,30(1):133-137. DOI: 10.13346/j.mycosystema.2011.01.022.
[34]	张洋, 张书祥. 产黄酮银杏内共生真菌的分离及其发酵条件的优化[J]. 工业微生物, 2015(2):21-28.
	ZHANG Y, ZHANG S X. Separation of flavonoid-producing endophytic fungi from Ginkgo biloba L. and optimization of fermentation conditions [J]. Industrial Microbiology, 2015,45(2):21-28. DOI: 10.3969/j.issn.1001-6678.2015.02.004.
[35]	于荣敏, 赵鸿莲, 张辉, 等. 银杏细胞悬浮培养及其银杏内酯产生的研究[J]. 生物工程学报, 1999,15(2):209-210.
	YU R M, ZHAO H L, ZHANG H, et al. Studies on the cell suspension culture of Ginkgo biloba and its Metabolites-ginkgolides [J]. Chinese Journal of Biotechnology, 1999,15(2):209-210. DOI: 10.1088/0256-307X/16/12/025.
[36]	李治滢, 杨丽源, 周斌. 灯盏细辛内生真菌菌种分离及其分类鉴定[J]. 云南大学学报(自然科学版), 2003,25(1):66-68.
	LI Z Y, YANG L Y, ZHOU B. Study on endophytic fungi in Erigeron breviscapus Ⅰ: isolation and identification of endophytic fungi [J]. Journal of Yunnan University (Natural Sciences), 2003,25(1):66-68. DOI: 10.3321/j.issn:0258-7971.2003.01.017.
[37]	兰琪姬, 志勤, 顾爱国. 苦皮藤内生真菌中杀虫杀菌活性物质的初步研究[J]. 西北农林科技大学学报(自然科学版), 2004,32(10):79-84.
	LAN Q J, ZHI Q, GU A G. Studies on insecticidical and fungicidical components of endophytic fungis in Celastrus angulatus [J]. Northwest A & F University, 2004,32(10):79-84. DOI: 10.3321/j.issn:1671-9387.2004.10.019.
[38]	邱德有, 黄美娟, 方晓华, 等. 一种云南红豆杉内生菌的分离[J]. 真菌学报, 1994,13(4):314-316.
	QIU D Y, HUANG M J, FANG X H, et al. Isolation of endophytic fungi from Yunnan taxus[J]. Acta Sinica, 1994,13(4):314-316. DOI: 10. 13346/j. mycosystema. 1994. 04. 015.
[39]	陆荣, 刘小娟, 樊美珍, 等. 喜树内生真菌的分离及其抗肿瘤活性菌株的筛选[J]. 安徽农业大学学报, 2008,35(1):76-79.
	LU R, LIU X J, FAN M Z, et al. Isolation of endophytic fungi from Camptotheca acuminate and screening test for strains of antitumor activity [J]. Journal of Anhui Agricultural University, 2008,35(1):76-79.
[40]	杨显志, 张玲琪, 郭波, 等. 一株产长春新碱内生真菌的初步研究[J]. 中草药, 2004,35(1):79-81.
	YANG X Z, ZHANG L Q, GUO B, et al. Preliminary study of a vincristine-producingendophytic fungus isolated from leaves of Catharanthus roseus [J]. Chinese Traditional and Herbal Drugs, 2004,35(1):79-81. DOI: 10.3321/j.issn:0253-2670.2004.01.033.
[41]	姜广策, 林永成, 周世宁. 中国南海红树内生真菌No.1403次级代谢物的研究[J]. 中山大学学报, 2000,39(6):65-72.
	JIANG G C, LIN Y C, ZHOU S N. Studies on the secondary metabolites of mangrove No.1403 from the south China sea[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2000,39(6):65-72. DOI: 10.3321/j.issn:0529-6579.2000.06.015.
[42]	李能章, 彭远义. 一株芦荟抗菌内生细菌的分离鉴定及生物学性质研究[J]. 生物技术通讯, 2004,15(2):141-145.
	LI N Z, PENG Y Y. Identification and characterization of an antimicrobial endophytic bacteria associated with Aloe chinensis (Haw.) Baker [J]. Letters in Biotechnology, 2004,15(2):141-145. DOI: 10.3969/j.issn.1009-0002.2004.02.012.
[43]	李姝诺, 李亚东, 王琦. 越橘产黄酮类化合物内生真菌的筛选[J]. 吉林农业大学学报, 2009,31(5):587-591.
	LI S N, LI Y D, WANG Q. Bolting flavonoid-producing endophytic fungi from Vaccinium [J]. Journal of Jilin Agriculture University, 2009,31(5):587-591. DOI: CNKI:SUN:JLNY.0.2009-05-025.
[44]	周松林, 史佳琴, 陈双林. 银杏内生真菌刺盘孢遗传多样性的初步分析[J]. 广西农业生物科学, 2006,25(2):145-149.
	ZHOU S L, SHI J Q, CHEN S L. Preliminary analysis of genetic diversity of endophytic fungus of Ginkgo biloba [J]. Journal of Guangxi Agricultural and Bioscience, 2006,25(2):145-149.
[45]	韩晓丽, 康冀川, 何劲, 等. 银杏产黄酮内生真菌的分离与鉴定[J]. 菌物研究, 2008,6(1):40-45.
	HAN X L, KANG J C, HE J, et al. Isolation and identification of endophytic fungi of falvonoid-producing Ginkgo biloba [J]. Journal of Fungal Research, 2008,6(1):40-45. DOI: 10.3969/j.issn.1672-3538.2008.01.005.
[46]	冷桂华, 刘紫英. 盘龙参内生真菌S-3产黄酮发酵条件的优化研究[J]. 食品工业, 2011,5:25-28.
	LENG G H, LIU Z Y. Study on fermentation conditions optimization of flavonoids-producing endophytic fungus S-3 from Spiranthes sinensis (Pers) Ames [J]. Food Industry, 2011,5:25-28. DOI: CNKI:SUN:SPGY.0.2011-05-010.

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纲classis	目order	科family	属genus	总量/株 total strain	分离频率/% separation frequency
			木霉属Trichoderma	13	19.4
			青霉属Penicillium	9	1.9
		丛梗孢科Moniliacea	拟青霉属Paecilomyces	4	6.0
			地霉属Geotrichum	2	4.5
半知菌纲Deuteromycetes	丛梗孢目Moniliales		链格孢属Alternaria	16	23.9
		束梗孢科Stilbellaceae	棒束梗霉属Stilbaceae	1	1.5
		瘤座孢科Tuberculariaceae	镰刀菌属Fusarium	10	14.9
	黑盘孢目Melanconiales	黑盘孢科Melanconiaceae	刺盘孢属Colletotrichum	6	8.9
丝孢菌纲Hyphomycetes	无孢菌目Agonomycetales	无孢菌科Agaricaceae	丝核菌属Rhizoctonia	1	1.5
结合菌纲Class Zygomycetes	毛霉目Mucorale	毛霉科Mucoraceae	根霉属Rhizoupus sp.	5	7.5

菌株 bacterial strain	异槲皮苷含量/ (μg·μL^-1) soquercitroside	槲皮素含量/ (μg·μL^-1) quercetin	山柰酚含量/ (μg·μL^-1) kaempferol	总黄酮含量/ (mg·L^-1) flavonoids of sample
CY12	0.016±0.003	0.021±0.006	0.076±0.054	1.42
PZ06	0.038±0.009	0.060±0.011	0.190±0.081	3.61
PP03	0.220±0.029	0	0	2.77
CP06	0.014±0.009	0.031±0.003	0	0.57

Isolation and identification of endophytic fungi from Cyclocarya paliurus (Batal.) Iljinskaja

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