JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (3): 117-122.doi: 10.12302/j.issn.1000-2006.202006050
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WU Lijun1,3,4(), YOU Yunfei2, CHEN Da1, CHEN Wenrong2, LI Wenfang2
Received:
2020-06-28
Revised:
2021-02-26
Online:
2021-05-30
Published:
2021-05-31
CLC Number:
WU Lijun, YOU Yunfei, CHEN Da, CHEN Wenrong, LI Wenfang. Optimization of the rooting and transplantation techinques of tissue-cultured shoots of Camellia chrysanthoides ‘Huangzun’[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 117-122.
Table 1
Effects of the combination of basic media and plant growth regulators on rooting rate"
培养基号 media No. | 基本培养基 basic media | 植物生长调节剂/(mg·L-1) plant growth regulators | 平均生根率/% average rooting rate | 芽苗生长情况 growth situation of shoots | |
---|---|---|---|---|---|
ABT | IBA | ||||
1 | 0 | 0 | 0.0±0 Ee | 芽苗未生根,开始黄化 | |
2 | 1/2 B5 | 0.5 | 0.5 | 42.2±2.94 Aa | 芽苗生长正常,主根短,须根少 |
3 | 1.0 | 1.0 | 41.1±1.10 Aa | 芽苗生长正常,主根短,须根少 | |
4 | 0 | 0.5 | 12.2±1.10 BCDcd | 芽苗高生长不明显,主根短、无须根 | |
5 | 3/4 B5 | 0.5 | 1.0 | 18.9±1.10 Bb | 芽苗高生长不明显,主根短、须根极少 |
6 | 1.0 | 0 | 18.9±2.20 Bb | 芽苗高生长不明显,主根短,须根少 | |
7 | 0 | 1.0 | 8.9±1.10 Dd | 芽苗矮壮,叶片变厚脆 | |
8 | B5 | 0.5 | 0 | 11.1±1.10 CDd | 芽苗矮壮,叶片变厚脆 |
9 | 1.0 | 0.5 | 16.7±1.93 BCbc | 芽苗矮壮,叶片变厚质化 | |
X1 | 27.77 7.07 9.63 | ||||
X2 | 16.67 23.70 23.70 | ||||
X3 | 11.87 25.57 22.97 | ||||
R | 15.90 18.50 14.07 |
Table 2
Effects of the combination of different culture substrates and additives on rooting rates"
组合号 combination No. | 培养基质与添加物 culture substrate and additives | 平均生根率/% average rooting rate | 芽苗生长情况 growth situation of shoots |
---|---|---|---|
① | 琼脂6.0 g/L +白糖0 g/L | 57.8±1.10 cC | 芽苗叶片淡绿、叶小,根细、短( |
② | 琼脂6.0 g/L +白糖15 g/L | 40.0±1.91 dD | |
③ | 琼脂6.0 g/L +白糖15 g/L+活性炭3.0 g/L | 33.3±1.93 eD | |
④ | 河沙蛭石+白糖0 g/L | 84.4±2.94 aA | 芽苗粗壮,叶片舒展,根系发达,主根平均2.8条以上,须根多( |
⑤ | 河沙蛭石+白糖15 g/L | 68.9±2.94 bB |
Table 3
Effects of the added sugar and K2HPO4 on rooting rates,transplanting survival rates and the yellow rate of shoots"
处理号 treatment No. | 处理 treatment | 平均 黄化率/% average yellow rate | 平均 生根率/% arerage rooting rate | 茎粗/mm diameter of shoots | 移栽 成活率/% transplanting survival rate | 芽苗生长情况 growth situation of shoots | |||
---|---|---|---|---|---|---|---|---|---|
平均 average | 最小 minimum | 最大 maximum | |||||||
50 d | 65 d | ||||||||
⑥ | 白糖15.0 g/L | 0 | 0 | 90.0±3.30 Aa | 1.63 | 1.32 | 2.13 | 90.1 Aa | 叶色淡绿 |
⑦ | K2HPO415.0 mg/L | 13.3 | 33.3 | 83.3±3.35 Aa | 1.71 | 1.36 | 2.16 | 42.7 Bb | 叶黄化、苗茎稍粗 |
⑧ | 白糖15.0 g/L+ K2HPO4 15.0 mg/L | 0 | 0 | 91.1±1.91 Aa | 2.16 | 1.82 | 2.80 | 93.9 Aa | 苗粗壮、叶片浓绿、根系发达( |
⑨ | CK(对照组) | 11.1 | 28.9 | 84.4±5.09 Aa | 1.61 | 1.45 | 1.96 | 35.5 Bb | 叶黄化、叶片易落 |
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