JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (3): 93-99.doi: 10.12302/j.issn.1000-2006.202009025
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LI Mengjuan1(), ZHU Liming1, HUO Junnan1, ZHANG Jingbo2, SHI Jisen3, CHENG Tielong1,*()
Received:
2020-09-11
Revised:
2021-02-22
Online:
2021-05-30
Published:
2021-05-31
Contact:
CHENG Tielong
E-mail:913500040@qq.com;ctielong@126.com
CLC Number:
LI Mengjuan, ZHU Liming, HUO Junnan, ZHANG Jingbo, SHI Jisen, CHENG Tielong. Cloning and expression analyses of NtCBL1,NtCBL2 gene of Nitraria tangutorum[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 93-99.
Table 1
The sequences of primers used in this study"
引物名称 primer | 序列(5'-3') sequence(5'-3') |
---|---|
NtCBL1-F | 5'-ATGGGGTGTTTTCAGTCAAA-3' |
NtCBL1-R | 5'-TTATGTGGCAAGCTCATCCA-3' |
NtCBL2-F | 5'-ATGGTGCAGTGCATAGAGGGA-3' |
NtCBL2-R | 5'-TCAGGTATCTTCAACTCGTGAATG-3' |
qCBL1-F | 5'-AGCGTGAAGAGGTCAAGCAA 3 |
qCBL1-R | 5'-ACCTGGTTTGCATCGGCTT-3' |
qCBL2-F | 5'-AGCTACACTTGCTGAGTCGG-3' |
qCBL2-R | 5'-ATGGATGTCGCAGGACAAGG |
β-Actin-F | 5'-CTTATAACGCTATCATAGGGCGTCTGAGC-3' |
β-Actin-R | 5'-TCGGTCAATATCTAGCCATGCGGG-3' |
gNtCBL1-F | 5'-ttccaccatggcgtgcaggtcgactctagaATGGGGTGGTT TTCAGTCAAAGGT-3' |
gNtCBL1-R | 5'-cagctcctcgcccttgctcaccatggatccTGTGGCAAGCTC ATCCACTTCT-3' |
gNtCBL2-F | 5'-ttccaccatggcgtgcaggtcgactctagaATGGTGCAGT GCATAGAGGGA-3' |
gNtCBL2-R | 5'-cagctcctcgcccttgctcaccatggatccGGTATCTTCAA CTCGTGAATGGAATACAAAAC-3' |
Fig.3
The multiple sequence alignment of NtCBL and CBL proteins from other plants A.NtCBL1与其他植物CBL1蛋白的多序列比对分析multiple sequence alignment of NtCBL1 and CBL1 proteins of other plants;AAC26008.1.拟南芥Arabidopsis thaliana;ABO43660.1.毛果杨Populus trichocarpa;AXM42381.1.木薯Manihot esculenta;KAF4362542.1.大麻Cannabis sativa;NP_001239047.1.番茄Solanum lycopersicum;ABC67906.1.胡杨Populus euphratica。B.NtCBL1与其他植物CBL1蛋白的多序列比对分析multiple sequence alignment of NtCBL2 and CBL2 proteins of other plants;ABW06389.1.陆地棉Gossypium hirsutum;AGM15885.1.杜梨Pyrus betulifolia;ABJ09704.1.胡杨Populus euphratica;AFK64728.1.百合Lilium longiflorum;ABO43661.1.毛果杨Populus trichocarpa。"
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