JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2023, Vol. 47 ›› Issue (2): 107-114.doi: 10.12302/j.issn.1000-2006.202110011
Previous Articles Next Articles
ZHANG Ganglong1,2(), ZHU Enyong3, LIU Liting1,*(), YANG Jiaqi2, WANG Yannan1, MO Xiaoyong2
Received:
2021-10-08
Revised:
2022-04-27
Online:
2023-03-30
Published:
2023-03-28
CLC Number:
ZHANG Ganglong, ZHU Enyong, LIU Liting, YANG Jiaqi, WANG Yannan, MO Xiaoyong. Optimization of the tissue culture technology system of Dicranopteris pedata[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(2): 107-114.
Table 2
Experiment Ⅲ: factors and the level design of orthogonal experiment for PGGB proliferation culture induction"
水平 level | 基本 培养基(C) basic culture media | 蔗糖 质量浓度 (D)/ (g·L-1) sucrose concentration | 6-BA 质量浓度 (E)/ (mg·L-1) 6-BA concentration | KT 质量浓度 (F)/ (mg·L-1) KT concentration |
---|---|---|---|---|
1 | MS | 5 | 0 | 0 |
2 | 1/2 MS | 15 | 0.5 | 0.5 |
3 | 1/4 MS | 30 | 1.0 | 1.0 |
Table 3
Experiment Ⅳ: orthogonal experiment for induction of sporophyte formation from mature prothallus"
水平 level | 基本 培养基(G) basic culture media | 蔗糖 质量浓度(H)/ (g·L-1) sucrose concentration | 6-BA 质量浓度 (I)/ (mg·L-1) 6-BA concentration | NAA 质量浓度 (J)/ (mg·L-1) NAA concentration |
---|---|---|---|---|
1 | MS | 5 | 0 | 0 |
2 | 1/2 MS | 15 | 0.5 | 0.5 |
3 | 1/4 MS | 30 | 1.0 | 1.0 |
Table 4
The analysis of variances of Scheirer-Ray-Hare test (Experiment Ⅱ)"
变异来源(因素) source of variation(foctor) | 平方和 sum of squares (SS) | 自由度 degree of freedom (df) | 均方 mean square (MS) | H统计量 H statistic | P |
---|---|---|---|---|---|
基本培养基(A) basic culture media | 55 229.804 | 3 | 18 409.935 | 72.60 | 0.000 |
消毒时间(B) disinfection time | 83.861 | 3 | 27.954 | 0.11 | 0.991 |
基本培养基×消毒时间(A×B) basic culture media× disinfection time | 2 184.546 | 9 | 242.727 | 2.87 | 0.969 |
误差error | 14 012.125 | 79 | 177.369 | ||
总计total | 71 510.336 | 94 | 760.748 |
Table 5
Significant differences and multiple comparison of Kruskal-Wallis test for Experiment Ⅱ"
处理号 treatment No. | 因素水平 factor level | 评价指标 evaluating indicator | |||
---|---|---|---|---|---|
A | B | 萌发率/% germination rate | 最早萌发时间/d earliest germination time | 染菌瓶数 number of infected bottles | |
1 | 1 | 1 | 19.08±11.26 c | 27 | 0 |
2 | 1 | 2 | 42.39±16.81 bc | 26 | 0 |
3 | 1 | 3 | 47.32±13.43 bc | 26 | 0 |
4 | 1 | 4 | 19.99±12.43 c | 27 | 1 |
5 | 2 | 1 | 70.08±14.24 abc | 24 | 0 |
6 | 2 | 2 | 87.43±3.01 abc | 24 | 0 |
7 | 2 | 3 | 76.40±1.91 abc | 25 | 0 |
8 | 2 | 4 | 82.44±3.86 abc | 24 | 0 |
9 | 3 | 1 | 94.16±0.80 abc | 22 | 0 |
10 | 3 | 2 | 79.58±15.42 abc | 24 | 0 |
11 | 3 | 3 | 92.89±1.28 abc | 22 | 0 |
12 | 3 | 4 | 94.74±0.56 abc | 22 | 0 |
13 | 4 | 1 | 97.15±0.69 a | 21 | 0 |
14 | 4 | 2 | 95.44±0.93 ab | 21 | 0 |
15 | 4 | 3 | 96.54±0.95 a | 20 | 0 |
16 | 4 | 4 | 97.08±0.68 a | 21 | 0 |
K1 | 32.20 | 70.12 | |||
K2 | 79.09 | 76.27 | |||
K3 | 90.34 | 78.29 | |||
K4 | 96.55 | 73.56 | |||
R | 64.35 | 8.17 |
Table 6
The nalysis of variance of Scheirer-Ray-Hare test for Experiment Ⅲ"
变异来源(因素) fact or(source of variation) | 平方和 SS | df | 均方 MS | H统计量 H statistic | P |
---|---|---|---|---|---|
基本培养基(C) basic culture media | 6 242.709 | 2 | 3 121.354 | 8.086 | 0.018 |
蔗糖(D)sucrose | 8 800.858 | 2 | 4 400.429 | 11.399 | 0.003 |
6-BA(E) | 23 703.507 | 2 | 11 851.753 | 30.702 | 0.000 |
KT(F) | 2 312.997 | 2 | 1 156.499 | 2.996 | 0.224 |
误差error | 37 688.977 | 94 | 400.947 | ||
总计total | 78 749.048 | 102 | 772.049 |
Table 7
Significant differences and multiple comparisons of Kruskal-Wallis nonparametric test for Experiment Ⅲ"
处理号 treatment No. | 因素水平 factor level | 评价指标 evaluating indicator | |||
---|---|---|---|---|---|
C | D | E | F | 30天后质量 增殖倍数 mass proliferation times in 30 days | |
1 | 1 | 1 | 1 | 1 | 2.60±0.22 abc |
2 | 2 | 1 | 2 | 2 | 7.28±0.60 a |
3 | 3 | 1 | 3 | 3 | 2.03±0.08 cd |
4 | 1 | 2 | 2 | 3 | 3.06±0.34 ab |
5 | 2 | 2 | 3 | 1 | 3.29±0.81 abcd |
6 | 3 | 2 | 1 | 2 | 2.16±0.13 bcd |
7 | 1 | 3 | 3 | 2 | 1.66±0.07 d |
8 | 2 | 3 | 1 | 3 | 2.13±0.06 bcd |
9 | 3 | 3 | 2 | 1 | 2.57±0.13 abc |
K1 | 2.44 | 3.97 | 2.29 | 2.82 | |
K2 | 4.23 | 2.84 | 4.30 | 3.70 | |
K3 | 2.25 | 2.12 | 2.32 | 2.41 | |
R | 1.98 | 1.85 | 2.01 | 1.29 |
Table 8
The analysis of variance results of Experiment Ⅳ for induction of sporophyte formation from mature prothallus"
变异来源(因素) source of variation | 平方和 SS | df | 均方 MS | F | P |
---|---|---|---|---|---|
基本培养基(G) basic culture media | 0.173 | 2 | 0.086 | 3.736 | 0.045 |
蔗糖sucrose(H) | 0.031 | 2 | 0.015 | 0.663 | 0.528 |
6-BA(I) | 0.240 | 2 | 0.120 | 5.177 | 0.018 |
NAA(J) | 0.122 | 2 | 0.061 | 2.627 | 0.101 |
误差error | 0.393 | 17 | 0.23 |
Table 9
Results of Duncan multiple comparative analysis for Experiment Ⅳ"
处理号 treatment No. | 因素水平 factor level | 评价指标 evaluating indicator | ||||
---|---|---|---|---|---|---|
G | H | I | J | 孢子体生成率/% sporophyte formation rate | 最早生成时间/d the earliest formation time | |
1 | 1 | 1 | 1 | 1 | 10.00±10.00 bc | 15 |
2 | 2 | 1 | 2 | 2 | 33.33±6.67 abc | 12 |
3 | 3 | 1 | 3 | 3 | 20.00±11.55 bc | 14 |
4 | 1 | 2 | 2 | 3 | 20.00±0.00 bc | 15 |
5 | 2 | 2 | 3 | 1 | 6.70±6.67 c | 16 |
6 | 3 | 2 | 1 | 2 | 40.00±11.55 ab | 10 |
7 | 1 | 3 | 3 | 2 | 6.70±6.67 c | 16 |
8 | 2 | 3 | 1 | 3 | 53.33±6.67 a | 8 |
9 | 3 | 3 | 2 | 1 | 26.67±13.33 abc | 13 |
K1 | 12.2 | 21.1 | 34.4 | 14.5 | ||
K2 | 31.1 | 22.2 | 26.7 | 26.7 | ||
K3 | 28.9 | 28.9 | 11.1 | 31.1 | ||
R | 18.9 | 7.8 | 23.3 | 16.6 |
[1] | 刘丽婷. 芒萁克隆种群格局形成的生态过程研究[D]. 北京: 北京林业大学, 2017. |
LIU L T. Analysis on population pattern of Dicranopteris pedata during spread process[D]. Beijing: Beijing Forestry University, 2017. | |
[2] | 王立丰, 季红兵, 田维敏. 重稀土矿区芒萁稀土元素精细定位及光抑制对其光合活性的影响[J]. 中国稀土学报, 2010, 28(3):379-386. |
WANG L F, JI H B, TIAN W M. Fine location of rare earths and effects of light inhibition on photosynthetic characterization of D.dichotoma in a heavy rare earths mine[J]. J Chin Rare Earth Soc, 2010, 28(3):379-386. | |
[3] | LIU L T, WANG X D, WEN Q, et al. Interspecific associations of plant populations in rare earth mining wasteland in Southern China[J]. Int Biodeterior Biodegrad, 2017, 118:82-88.DOI: 10.1016/j.ibiod.2017.01.011. |
[4] | 焦瑜, 王晖, 张寿洲. 蕨类植物图谱:孢子体和原叶体[M]. 北京: 中国林业出版社, 2014:29. |
JIAO Y, WANG H, ZHANG S Z. Album of pteridophytes[M]. Beijing: China Forestry Publishing House, 2014:29. | |
[5] | 张静, 蔡静如, 许建新, 等. 蕨类植物在生态修复中的应用研究进展[J]. 安徽农业科学, 2017, 45(4):69-71,109. |
ZHANG J, CAI J R, XU J X, et al. Application research progress of ferns in ecological restoration[J]. J Anhui Agric Sci, 2017, 45(4):69-71,109.DOI: 10.13989/j.cnki.0517-6611.2017.04.023. | |
[6] | 林夏馨. 芒萁的生长发育规律及人工繁殖技术[J]. 福建水土保持, 2004, 16(2):60-62. |
LIN X X. Growth and development law and artificial propagation technology of Dicranopteris dichotoma[J]. Fujian Soil Water Conserv, 2004, 16(2):60-62.DOI: 10.3969/j.issn.1002-2651.2004.02.017. | |
[7] | 张煜, 张旭, 邱子豪, 等. 芒萁的生态学与资源利用研究进展[J]. 草业科学, 2021, 38(8):1525-1536. |
ZHANG Y, ZHANG X, QIU Z H, et al. Ecological effect of Dicranopteris pedata and its resource utilization:a review[J]. Pratacultural Sci, 2021, 38(8):1525-1536.DOI: 10.11829/j.issn.1001-0629.2021-0039. | |
[8] | 吴丽君, 游云飞, 陈达, 等. ‘黄樽’薄叶金花茶组培苗生根与移栽技术研究[J]. 南京林业大学学报(自然科学版), 2021, 45(3):117-122. |
WU L J, YOU Y F, CHEN D, et al. Optimization of the rooting and transplantation techinques of tissue-cultured shoots of Camellia chrysanthoides ‘Huangzun’[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(3):117-122.DOI: 10.12302/j.issn.1000-2006.202006050. | |
[9] | 李杨, 余蓉培, 李慧, 等. 观赏蕨类植物组织培养研究进展[J]. 园艺学报, 2012, 39(9):1839-1848. |
LI Y, YU R P, LI H, et al. A review of the development in tissue culture of ornamental ferns[J]. Acta Hortic Sin, 2012, 39(9):1839-1848.DOI: 10.16420/j.issn.0513-353x.2012.09.023. | |
[10] | 张焕玲. 金叶复叶槭水培嫩枝组织培养体系研究[J]. 南京林业大学学报(自然科学版), 2022, 46(5):89-94. |
ZHANG H L. Study on tissue culture system of Acer negundo ‘Aurea’ using hydroponic new shoots as explants[J]. J Nanjing For Univ (Nat Sci Ed), 2022, 46(5):89-94.DOI:10.12302/j.issn.1000-2006.202109021. | |
[11] | 罗士韦, 许智宏. 经济植物组织培养[M]. 北京: 科学出版社, 1988. |
LUO S W, XU Z H. Tissue culture of economic plants[M]. Beijing: Science Press, 1988. | |
[12] | 欧阳磊, 郑仁华, 翁玉榛, 等. 杉木优良无性系组培快繁技术体系的建立[J]. 南京林业大学学报(自然科学版), 2007, 31(3):47-51. |
OUYANG L, ZHENG R H, WENG Y Z, et al. Establishment of technique system of tissue culture on Chinese fir superior clones[J]. J Nanjing For Univ (Nat Sci Ed), 2007, 31(3):47-51.DOI: 10.3969/j.issn.1000-2006.2007.03.010. | |
[13] | 杨春梅, 屈云慧, 汪国鲜, 等. 芒萁组织培养和快繁技术研究[J]. 西南农业学报, 2016, 29(12):2955-2958. |
YANG C M, QU Y H, WANG G X, et al. Study on tissue culture and rapid propagation technique of Dicranopteris dichotoma hance meristem[J]. Southwest China J Agric Sci, 2016, 29(12):2955-2958.DOI: 10.16213/j.cnki.scjas.2016.12.033. | |
[14] | 吴远彬. 芒萁人工培育技术研究初报[J]. 亚热带水土保持, 2016, 28(2):8-11,38. |
WU Y B. Initial study report on the artificial culture techniques of Dicranopteris dichotoma(Thunb.) Bernh[J]. Subtrop Soil Water Conserv, 2016, 28(2):8-11,38. | |
[15] | 申希平, 祁海萍, 刘小宁, 等. 两因素非参数方差分析在SPSS中的实现[J]. 中国卫生统计, 2013, 30(6):913-914. |
SHEN X P, QI H P, LIU X N, et al. Realization of two-factor nonparametric variance analysis in SPSS[J]. Chin J Health Stat, 2013, 30(6):913-914. | |
[16] | 蒲虎. Kruskal-Wallis检验原理介绍及其应用[J]. 兴义民族师范学院学报, 2019(4):108-111. |
PU H. Introduction and application of Kruskal-Wallis test principle[J]. J Xingyi Norm Univ Natl, 2019(4):108-111.DOI: 10.3969/j.issn.1009-0673.2019.04.022. | |
[17] | 王一诺, 韦莹, 李翠, 等. 植物生长调节素对西南凤尾蕨组织培养的影响[J]. 湖北农业科学, 2016, 55(8):2117-2119. |
WANG Y N, WEI Y, LI C, et al. Effects of plant growth regulators on tissue culture of Pteris wallichianain[J]. Hubei Agric Sci, 2016, 55(8):2117-2119.DOI: 10.14088/j.cnki.issn0439-8114.2016.08.052. | |
[18] | 吴长辉. 里白孢子体快繁关键技术研究[D]. 杭州: 浙江农林大学, 2012. |
WU C H. White spores in micropropagation of key technology research[D]. Hangzhou: Zhejiang A & F University, 2012. | |
[19] | 黄弄璋, 张梦君, 皮嘉雯, 等. 月季外植体的消毒及启动培养研究[J]. 现代园艺, 2021, 44(13):40-41. |
HUANG L Z, ZHANG M J, PI J W, et al. Study on disinfection and start-up culture of rose explants[J]. Contemp Hortic, 2021, 44(13):40-41.DOI: 10.14051/j.cnki.xdyy.2021.13.015. | |
[20] | 余蓉培, 李杨, 李东, 等. 荚果蕨绿色球状体对60Coγ射线的辐射敏感性[J]. 植物学报, 2015, 50(5):565-572. |
YU R P, LI Y, LI D, et al. Radiosensitivity of green globular bodies of Matteuccia struthiopteris exposed to 60Coγ radiation[J]. Chin Bull Bot, 2015, 50(5):565-572.DOI: 10.11983/CBB14141. | |
[21] | 程栋梁, 钟全林, 林茂兹, 等. 植物代谢速率与个体生物量关系研究进展[J]. 生态学报, 2011, 31(8):2312-2320. |
CHENG D L, ZHONG Q L, LIN M Z, et al. The advance of allometric studies on plant metabolic rates and biomass[J]. Acta Ecol Sin, 2011, 31(8):2312-2320. | |
[22] | CHEN X S, LI Y F, XIE Y H, et al. Trade-off between allocation to reproductive ramets and rhizome buds in Carex brevicuspis populations along a small-scale elevational gradient[J]. Sci Rep, 2015, 5:12688.DOI: 10.1038/srep12688. |
[23] | 焦德志, 刘润泽, 潘林, 等. 扎龙湿地不同生长期芦苇分株构件生物量分配及生长分析[J]. 生态学杂志, 2021, 40(4):940-949. |
JIAO D Z, LIU R Z, PAN L, et al. Biomass allocation and growth analysis of the ramets of Phragmites communis in different growth stages in Zhalong wetland[J]. Chin J Ecol, 2021, 40(4):940-949.DOI: 10.13292/j.1000-4890.202104.001. | |
[24] | LOESCHER W H, ALBRECHT C N. Development in vitro of Nephrolepis exaltata cv. bostoniensis runner tissues[J]. Physiol Plant, 1979, 47(4):250-254.DOI: 10.1111/j.1399-3054.1979.tb06522.x. |
[25] | 张义, 刘云利, 刘子森, 等. 植物生长调节剂的研究及应用进展[J]. 水生生物学报, 2021, 45(3):700-708. |
ZHANG Y, LIU Y L, LIU Z S, et al. The research and application progress of plant growth regulators[J]. Acta Hydrobiol Sin, 2021, 45(3):700-708.DOI: 10.7541/2021.2019.035. | |
[26] | 黄武杰, 曹建国, 王全喜. 蕨配子体发育及卵发生的显微结构观察[J]. 植物研究, 2011, 31(2):159-163. |
HUANG W J, CAO J G, WANG Q X. Microstructural observations on the development of gametophytes and oogenesis in the fern Pteridium aquilinum var. latiusculum[J]. Bull Bot Res, 2011, 31(2):159-163. |
[1] | WANG Lingjian, JIA Zhaohui, ZHANG Jinchi, TANG Xinggang, SUN Xin, MENG Miaojing, LIU Xin. Optimization for fermentation conditions and analysis of application effect for high efficiency dissolution strain Bt NL-11 from Bacillus thuringiensis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(3): 71-80. |
[2] | SA Rula, WANG Zirui, HUA Yongchun, HU Richa, LIU Lei, GAO Minglong, YU Xiaoyu. Evaluating forest ecosystem restoration ability of natural forest in northern Greater Khingan Mountains by a structural equation model [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(1): 196-204. |
[3] | LI Wei, LI Jiping, ZHANG Yinlong, LI Pingping, HAN Jiangang. Ecological restoration technologies for lake wetlands for carbon peaking and neutrality [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 157-166. |
[4] | ZHANG Jinchi, LI Chong, JIA Zhaohui, LIU Xin, MENG Miaojing. Application of functional microorganisms in ecological restoration of abandoned mines [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(6): 146-156. |
[5] | XU Haishun, DU Hongyu, CAI Chaolin. Spatial characteristics of landscape pattern based on ecological restoration in the riparian zone of Huangpu River, Shanghai City [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(04): 125-131. |
[6] | YUAN Fang, CHEN Huaiyan, KANG Xin, SHENG Sheng, XU Chi, LIU Maosong. Relationship between shoreline morphology and elements distribution in near-shore sediments [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(02): 9-14. |
[7] | MAO Xuegang, WANG Hang. Assessing ecological and environmental vulnerability of Miyun County in Beijing according to pixel scale [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2017, 41(01): 96-102. |
[8] | CAO Jiajie, LU Jun’an, RUAN Honghua. Effects of underwater illumination compensation on growth and physiological indices of submerged macrophyte Vallisneria natans L. [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2014, 38(05): 102-106. |
[9] | XU Xinzhou,XUE Jianhui,LYU Zhigang,LIU Chunlai. A research of ecological function area and vegetation restoration at Taihu Gonghu bay wetland [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2013, 37(03): 35-40. |
[10] | FENG Yuqing1,2, WANG Ying1, RUAN Honghua1*. A review of the researches on riparian zones [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2009, 33(06): 127-. |
[11] | FENG Yuqing1,2, WANG Shaojun1*, RUAN Honghua1, ZHANG Yinlong1, GUAN Qingwei1, XU Zhen1. Ecological restoration and strategy on a riparian zone at Taihu Lake in Suzhou City [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2009, 33(05): 126-. |
[12] | HAN Weidong,LING Dajiong,LI Yan,WU XiaofengAgricultural College Zhanjiang Ocean University, Zhanjiang 524088,China). The Soil Dynamic Study of Restored Sonneratia apetala Plantations [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2003, 27(02): 49-54. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||