芒萁组培技术体系的优化

张港隆; 朱恩永; 刘丽婷; 杨嘉麒; 汪雁楠; 莫晓勇

doi:10.12302/j.issn.1000-2006.202110011

芒萁组培技术体系的优化

张港隆, 朱恩永, 刘丽婷, 杨嘉麒, 汪雁楠, 莫晓勇

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2) : 107-114.

PDF(2252 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2252 KB)

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2) : 107-114. DOI: 10.12302/j.issn.1000-2006.202110011

研究论文

芒萁组培技术体系的优化

张港隆 ¹^,² ,
朱恩永 ³ ,
刘丽婷 ¹^,^* ,
杨嘉麒 ² ,
汪雁楠 ¹ ,
莫晓勇 ²

作者信息 +

Optimization of the tissue culture technology system of Dicranopteris pedata

ZHANG Ganglong ¹^,² ,
ZHU Enyong ³ ,
LIU Liting ¹^,^* ,
YANG Jiaqi ² ,
WANG Yannan ¹ ,
MO Xiaoyong ²

Author information +

文章历史 +

摘要

【目的】芒萁(Dicranopteris pedate)是尾矿及酸性边坡修复的优良植物。优化芒萁组培技术,提高组培效率,可为困难立地修复提供大量芒萁孢子体。【方法】以芒萁孢子为外植体,研究外植体收集时间、消毒时间与孢子萌发率的关系;通过改变培养基中无机盐浓度、蔗糖浓度及植物生长调节剂类型及浓度,研究孢子萌发、原叶体增殖、孢子体生成3个过程的最佳培养基配方。【结果】春季2—4月采集的芒萁孢子萌发活性最强;孢子诱导萌发最佳处理为DKW +5%(质量分数) NaClO消毒15 s,萌发率97.15%,20 d原叶体可见;原叶体最佳增殖培养基为1/2 MS+蔗糖5 g/L + 6-BA 0.5 mg/L + KT 0.5 mg/L,30 d质量增殖倍数为7.28;孢子体诱导生成最佳培养基为1/2 MS+蔗糖30 g/L + NAA 1.0 mg/L,第8天孢子体即可产生,生成率53.33%。【结论】芒萁孢子活性与生长区域、生成月份存在关联性,广州地区3月的孢子活性最强;在幼孢子体的瓶苗生成率较低的情况下,研发原叶体绿色球状体(prothallus green globular body, PGGB)高效增殖为核心的芒萁苗繁育技术,有助于实现芒萁苗大量、快速生产。

Abstract

【Objective】 Optimizing tissue culture techniques of Dicranopteris pedata is needed to improve the success rate and productivity of tissue culture.【Method】 Dicranopteris pedata spores were used as explants to study the relationship between the explant collection time, disinfection time and spore germination rate. By using the different concentration of inorganic salt and sucrose, along with the type and concentration of the plant growth regulator in the medium, the optimal medium formula for the spore germination, prothallus proliferation and sporophyte formation were studied.【Result】 March was the optimal time for collecting spores; the optimal treatment for inducing spore germination was DKW + NaClO (5%) disinfection for 15 s, with which the germination rate was 97.15%. Prothallus appeared after 20 days and the optimal medium for prothallus proliferation was 1/2 MS + sucrose 5 g/L + 6-BA 0.5 mg/L + KT 0.5 mg/L. The mass proliferation multiple was 7.28 after 30 days; the optimal medium for sporophyte induction was 1/2 MS + sucrose 30 g/L + NAA 1.0 mg/L; sporophytes appeared after 8 days, and the formation rate was 53.33%.【Conclusion】 Spore activity of Dicranopteris pedata is related to the growth area and formation month, and the activity of spores is strongest in Guangzhou in March. In the case of low bottle seedling rates of young sporophytes, the seedling breeding technology with efficient proliferation of prothallus green globular body as the core has been developed, which will contribute to the realization of large-scale and rapid production of Dicranopteris pedata seedlings.

导出引用

张港隆, 朱恩永, 刘丽婷, 等. 芒萁组培技术体系的优化[J]. 南京林业大学学报（自然科学版）. 2023, 47(2): 107-114 https://doi.org/10.12302/j.issn.1000-2006.202110011

ZHANG Ganglong, ZHU Enyong, LIU Liting, et al. Optimization of the tissue culture technology system of Dicranopteris pedata[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2023, 47(2): 107-114 https://doi.org/10.12302/j.issn.1000-2006.202110011

中图分类号： S722.3+7;Q914.85

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]	刘丽婷. 芒萁克隆种群格局形成的生态过程研究[D]. 北京: 北京林业大学, 2017. LIU L T. Analysis on population pattern of Dicranopteris pedata during spread process[D]. Beijing: Beijing Forestry University, 2017. 本文引用 [3]

[2]

王立丰, 季红兵, 田维敏. 重稀土矿区芒萁稀土元素精细定位及光抑制对其光合活性的影响[J]. 中国稀土学报, 2010, 28(3):379-386.

WANG

L F

, JI

H B

, TIAN

W M

. Fine location of rare earths and effects of light inhibition on photosynthetic characterization of D.dichotoma in a heavy rare earths mine[J]. J Chin Rare Earth Soc, 2010, 28(3):379-386.

本文引用 [1]

[3]	LIU L T, WANG X D, WEN Q, et al. Interspecific associations of plant populations in rare earth mining wasteland in Southern China[J]. Int Biodeterior Biodegrad, 2017, 118:82-88.DOI: 10.1016/j.ibiod.2017.01.011. 本文引用 [1]

[4]	焦瑜, 王晖, 张寿洲. 蕨类植物图谱:孢子体和原叶体[M]. 北京: 中国林业出版社, 2014:29. JIAO Y, WANG H, ZHANG S Z. Album of pteridophytes[M]. Beijing: China Forestry Publishing House, 2014:29. 本文引用 [1]

[5]	张静, 蔡静如, 许建新, 等. 蕨类植物在生态修复中的应用研究进展[J]. 安徽农业科学, 2017, 45(4):69-71,109. ZHANG J, CAI J R, XU J X, et al. Application research progress of ferns in ecological restoration[J]. J Anhui Agric Sci, 2017, 45(4):69-71,109.DOI: 10.13989/j.cnki.0517-6611.2017.04.023. 本文引用 [1]

[6]	林夏馨. 芒萁的生长发育规律及人工繁殖技术[J]. 福建水土保持, 2004, 16(2):60-62. LIN X X. Growth and development law and artificial propagation technology of Dicranopteris dichotoma[J]. Fujian Soil Water Conserv, 2004, 16(2):60-62.DOI: 10.3969/j.issn.1002-2651.2004.02.017. 本文引用 [1]

[7]	张煜, 张旭, 邱子豪, 等. 芒萁的生态学与资源利用研究进展[J]. 草业科学, 2021, 38(8):1525-1536. ZHANG Y, ZHANG X, QIU Z H, et al. Ecological effect of Dicranopteris pedata and its resource utilization:a review[J]. Pratacultural Sci, 2021, 38(8):1525-1536.DOI: 10.11829/j.issn.1001-0629.2021-0039. 本文引用 [1]

[8]

吴丽君, 游云飞, 陈达, 等. ‘黄樽’薄叶金花茶组培苗生根与移栽技术研究[J]. 南京林业大学学报(自然科学版), 2021, 45(3):117-122.

L J

, YOU

Y F

, CHEN

, et al. Optimization of the rooting and transplantation techinques of tissue-cultured shoots of Camellia chrysanthoides ‘Huangzun’[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(3):117-122.DOI: 10.12302/j.issn.1000-2006.202006050.

本文引用 [1]

[9]	李杨, 余蓉培, 李慧, 等. 观赏蕨类植物组织培养研究进展[J]. 园艺学报, 2012, 39(9):1839-1848. LI Y, YU R P, LI H, et al. A review of the development in tissue culture of ornamental ferns[J]. Acta Hortic Sin, 2012, 39(9):1839-1848.DOI: 10.16420/j.issn.0513-353x.2012.09.023. 本文引用 [1]

[10]	张焕玲. 金叶复叶槭水培嫩枝组织培养体系研究[J]. 南京林业大学学报(自然科学版), 2022, 46(5):89-94. ZHANG H L. Study on tissue culture system of Acer negundo ‘Aurea’ using hydroponic new shoots as explants[J]. J Nanjing For Univ (Nat Sci Ed), 2022, 46(5):89-94.DOI:10.12302/j.issn.1000-2006.202109021. 本文引用 [1]

[11]	罗士韦, 许智宏. 经济植物组织培养[M]. 北京: 科学出版社, 1988. LUO S W, XU Z H. Tissue culture of economic plants[M]. Beijing: Science Press, 1988. 本文引用 [1]

[12]

欧阳磊, 郑仁华, 翁玉榛, 等. 杉木优良无性系组培快繁技术体系的建立[J]. 南京林业大学学报(自然科学版), 2007, 31(3):47-51.

OUYANG

, ZHENG

R H

, WENG

Y Z

, et al. Establishment of technique system of tissue culture on Chinese fir superior clones[J]. J Nanjing For Univ (Nat Sci Ed), 2007, 31(3):47-51.DOI: 10.3969/j.issn.1000-2006.2007.03.010.

本文引用 [1]

[13]

杨春梅, 屈云慧, 汪国鲜, 等. 芒萁组织培养和快繁技术研究[J]. 西南农业学报, 2016, 29(12):2955-2958.

YANG

C M

, QU

Y H

, WANG

G X

, et al. Study on tissue culture and rapid propagation technique of Dicranopteris dichotoma hance meristem[J]. Southwest China J Agric Sci, 2016, 29(12):2955-2958.DOI: 10.16213/j.cnki.scjas.2016.12.033.

本文引用 [4]

[14]	吴远彬. 芒萁人工培育技术研究初报[J]. 亚热带水土保持, 2016, 28(2):8-11,38. WU Y B. Initial study report on the artificial culture techniques of Dicranopteris dichotoma(Thunb.) Bernh[J]. Subtrop Soil Water Conserv, 2016, 28(2):8-11,38. 本文引用 [7]

[15]	申希平, 祁海萍, 刘小宁, 等. 两因素非参数方差分析在SPSS中的实现[J]. 中国卫生统计, 2013, 30(6):913-914. SHEN X P, QI H P, LIU X N, et al. Realization of two-factor nonparametric variance analysis in SPSS[J]. Chin J Health Stat, 2013, 30(6):913-914. 本文引用 [1]

[16]	蒲虎. Kruskal-Wallis检验原理介绍及其应用[J]. 兴义民族师范学院学报, 2019(4):108-111. PU H. Introduction and application of Kruskal-Wallis test principle[J]. J Xingyi Norm Univ Natl, 2019(4):108-111.DOI: 10.3969/j.issn.1009-0673.2019.04.022. 本文引用 [1]

[17]	王一诺, 韦莹, 李翠, 等. 植物生长调节素对西南凤尾蕨组织培养的影响[J]. 湖北农业科学, 2016, 55(8):2117-2119. WANG Y N, WEI Y, LI C, et al. Effects of plant growth regulators on tissue culture of Pteris wallichianain[J]. Hubei Agric Sci, 2016, 55(8):2117-2119.DOI: 10.14088/j.cnki.issn0439-8114.2016.08.052. 本文引用 [2]

[18]	吴长辉. 里白孢子体快繁关键技术研究[D]. 杭州: 浙江农林大学, 2012. WU C H. White spores in micropropagation of key technology research[D]. Hangzhou: Zhejiang A & F University, 2012. 本文引用 [1]

[19]	黄弄璋, 张梦君, 皮嘉雯, 等. 月季外植体的消毒及启动培养研究[J]. 现代园艺, 2021, 44(13):40-41. HUANG L Z, ZHANG M J, PI J W, et al. Study on disinfection and start-up culture of rose explants[J]. Contemp Hortic, 2021, 44(13):40-41.DOI: 10.14051/j.cnki.xdyy.2021.13.015. 本文引用 [1]

[20]	余蓉培, 李杨, 李东, 等. 荚果蕨绿色球状体对⁶⁰Coγ射线的辐射敏感性[J]. 植物学报, 2015, 50(5):565-572. YU R P, LI Y, LI D, et al. Radiosensitivity of green globular bodies of Matteuccia struthiopteris exposed to ⁶⁰Coγ radiation[J]. Chin Bull Bot, 2015, 50(5):565-572.DOI: 10.11983/CBB14141. 本文引用 [1]

[21]	程栋梁, 钟全林, 林茂兹, 等. 植物代谢速率与个体生物量关系研究进展[J]. 生态学报, 2011, 31(8):2312-2320. CHENG D L, ZHONG Q L, LIN M Z, et al. The advance of allometric studies on plant metabolic rates and biomass[J]. Acta Ecol Sin, 2011, 31(8):2312-2320. 本文引用 [1]

[22]	CHEN X S, LI Y F, XIE Y H, et al. Trade-off between allocation to reproductive ramets and rhizome buds in Carex brevicuspis populations along a small-scale elevational gradient[J]. Sci Rep, 2015, 5:12688.DOI: 10.1038/srep12688. 本文引用 [1]

[23]

焦德志, 刘润泽, 潘林, 等. 扎龙湿地不同生长期芦苇分株构件生物量分配及生长分析[J]. 生态学杂志, 2021, 40(4):940-949.

JIAO

D Z

, LIU

R Z

, PAN

, et al. Biomass allocation and growth analysis of the ramets of Phragmites communis in different growth stages in Zhalong wetland[J]. Chin J Ecol, 2021, 40(4):940-949.DOI: 10.13292/j.1000-4890.202104.001.

本文引用 [1]

[24]	LOESCHER W H, ALBRECHT C N. Development in vitro of Nephrolepis exaltata cv. bostoniensis runner tissues[J]. Physiol Plant, 1979, 47(4):250-254.DOI: 10.1111/j.1399-3054.1979.tb06522.x. 本文引用 [1]

[25]	张义, 刘云利, 刘子森, 等. 植物生长调节剂的研究及应用进展[J]. 水生生物学报, 2021, 45(3):700-708. ZHANG Y, LIU Y L, LIU Z S, et al. The research and application progress of plant growth regulators[J]. Acta Hydrobiol Sin, 2021, 45(3):700-708.DOI: 10.7541/2021.2019.035. 本文引用 [1]

[26]	黄武杰, 曹建国, 王全喜. 蕨配子体发育及卵发生的显微结构观察[J]. 植物研究, 2011, 31(2):159-163. HUANG W J, CAO J G, WANG Q X. Microstructural observations on the development of gametophytes and oogenesis in the fern Pteridium aquilinum var. latiusculum[J]. Bull Bot Res, 2011, 31(2):159-163. 本文引用 [1]