Analyses of dynamic growth traits of the stecklings from the F1 hybrid progeny of Populus deltoides × P. simonii

doi:10.12302/j.issn.1000-2006.201907030

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (1): 45-52.doi: 10.12302/j.issn.1000-2006.201907030

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Analyses of dynamic growth traits of the stecklings from the F₁hybrid progeny of Populus deltoides × P. simonii

CHEN Yuhua¹^,²(), YAO Dan¹, WU Hainan¹, TAO Shengtong¹, WU Jiyan¹, YANG Wenguo¹, TONG Chunfa¹^,^*()

1. Co-Innovation Center for the Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. School of Animal Science and Technology, Jingling Institute of Technology, Nanjing 210038, China

Received:2019-07-22 Accepted:2019-11-18 Online:2021-01-30 Published:2021-02-01
Contact: TONG Chunfa E-mail:cyh@jit.edu.cn;tongchf@njfu.edu.cn

Abstract

Abstract:

【Objective】 This study aimed to investigate the variation in the growth traits of clones from the F₁ progeny of Populus deltoides × P. simonii to facilitate the establishment of statistical models for detecting quantitative trait loci (QTL) using such clonal materials. 【Method】 A randomized block experiment was designed to test clones from the F₁ hybrid population. Tree height and ground diameter were measured for each clonal individual at multiple time points in 2017 and 2018. The statistical software SPSS was used to perform the variation analysis of the growth traits with linear mixed models, while an R package was used for a clustering analysis of clonal effects estimated from the linear models. 【Result】 The effects of tree height and ground diameter were significantly different among the blocks, among the clones, and among the interactions of blocks and clones. The phenotypic coefficient of variation for tree height varied from 31.96% to 22.04% in 2017, and this index was maintained at 20% in 2018. The coefficient of variation for ground diameter was 26.66% and 26.20% in 2017 and 2018, respectively. The repeatability of both tree height and ground diameter varied at multiple time points in the two years. The repeatability of tree height gradually decreased from 0.69 to 0.41 in 2017 but increased to nearly 0.60 in 2018. The repeatability of ground diameter was low in 2017 but increased to 0.59 in 2018. According to the estimates of clonal effects at different time points, the clones were roughly clustered into eight groups, in each of which the mean effects varied uniquely over time. The clonal mean effects for the four clusters in 2017 reduced over time, but for the other four clusters, the means increased. Although the mean effects within clusters in 2018 varied smoothly, the differences between clusters expanded. 【Conclusion】 In summary, the tree height and ground diameter of the clones in the randomized block experiment displayed a great variation, especially at the genetic level at different growth stages over the two years. This provides important evidence for creating unique statistical models for mapping QTLs using these clonal experimental materials.

Key words: Populus, clone, randomized block experiment, repeatability, clustering analysis, dynamic growth at steckling stage

CLC Number:

S722.3
Q348

CHEN Yuhua, YAO Dan, WU Hainan, TAO Shengtong, WU Jiyan, YANG Wenguo, TONG Chunfa. Analyses of dynamic growth traits of the stecklings from the F₁hybrid progeny of Populus deltoides × P. simonii[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(1): 45-52.

Figures/Tables 5

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Table 1

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数据集 dataset	效应 effect	?统计量 Wilks' Lambda	F统计量 F statistics	假设自由度 hypothesis df	误差自由度 error df
2017年苗高 tree height in 2017	区组	0.350	109.050	16	2 524.000
	无性系	0.011	5.022	1 512	10 108.023
	区组×无性系	0.011	2.529	3 024	10 114.597
2018年苗高 tree height in 2018	区组	0.587	46.099	14	2 112.000
	无性系	0.073	3.453	973	7 398.012
	区组×无性系	0.037	2.296	1 946	7 404.611
2017—2018年地径 ground diameter across 2017 to 2018	区组	0.746	71.261	4	1 806.000
	无性系	0.320	5.027	276	1 806.000
	区组×无性系	0.330	2.467	542	1 806.000

性状 traits	平均值/cm average	标准差/cm SD	变异范围/cm range	变异系数/% CV	重复力 repeatability
H(20170608)	34.67	11.08	6.50~83.20	31.96	0.689
H(20170623)	57.54	17.73	11.90~129.50	30.81	0.630
H(20170706)	80.94	23.13	20.50~167.00	28.58	0.610
H(20170716)	99.48	26.69	31.00~195.00	26.83	0.594
H(20170727)	120.41	30.52	35.00~234.00	25.35	0.587
H(20170814)	154.34	35.32	45.00~258.00	22.88	0.515
H(20170902)	202.66	44.16	54.00~327.00	21.79	0.442
H(20171014)	219.16	48.30	56.00~347.00	22.04	0.412
H(20180524)	243.39	53.71	37.00~365.00	22.07	0.398
H(20180621)	266.58	53.06	52.00~380.00	19.90	0.436
H(20180707)	281.25	54.76	60.00~455.00	19.47	0.466
H(20180724)	291.94	56.49	84.00~490.00	19.34	0.499
H(20180814)	300.34	58.03	85.00~500.00	19.32	0.553
H(20180909)	307.56	59.27	85.00~500.00	19.27	0.578
H(20181027)	317.44	60.97	95.00~520.00	19.21	0.582
D(20171015)	1.50	0.40	0.25~3.15	26.66	0.376
D(20181103)	2.02	0.53	0.77~4.35	26.20	0.594

2017 年	2018年									2017^*
2017 年	CL1	CL2	CL3	CL4	CL5	CL6	CL7	CL8	小计 total	2017^*
CL1	6	0	5	0	0	0	0	0	11	14
CL2	1	21	0	10	4	0	0	0	36	5
CL3	9	2	9	0	7	1	0	0	28	13
CL4	8	2	4	0	2	0	0	0	16	7
CL5	0	0	1	0	0	2	0	0	3	5
CL6	4	9	1	1	9	0	0	0	24	6
CL7	0	3	0	6	1	0	7	1	18	1
CL8	0	0	0	1	0	0	2	0	3	0
小计 total	28	37	20	18	23	3	9	1	139	51

Analyses of dynamic growth traits of the stecklings from the F₁hybrid progeny of Populus deltoides × P. simonii

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