Comparative study on the effects of nutrient solution treatment in hydroponic cultivation of Carpinus betulus

doi:10.12302/j.issn.1000-2006.201911011

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 17-24.doi: 10.12302/j.issn.1000-2006.201911011

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Comparative study on the effects of nutrient solution treatment in hydroponic cultivation of Carpinus betulus

HUO Yan¹(), ZHANG Huihui¹, ZHU Zunling¹^,²^,^*()

1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
2. College of Art and Design, Nanjing Forestry University, Nanjing 210037, China

Received:2019-11-11 Accepted:2020-12-21 Online:2021-03-30 Published:2021-04-09
Contact: ZHU Zunling E-mail:yanhuo@njfu.edu.cn;zhuzunling@njfu.edu.cn

Abstract

Abstract:

【Objective】To provide a theoretical basis for hydroponic breeding, production, and further physiological and biochemical studies of Carpinus betulus, the effects of type, concentration and pH of ordinary nutrient solution treatment and special nutrient solution for hydroponic seedling growth of C. betulus were examined, and the best nutrient solution for the growth of C. betulus seedlings was screened.【Method】We used C. betulus hydroponic seedlings as the test material. We analyzed the survival rate, number of leaves, average number of roots, average number of second roots and fresh root weight as morphological indexes; and soluble sugar content, starch content, soluble protein content, POD activity and root activity as physical indexes. Hydroponic seedling growth was examined by the type, concentration and pH value of nutrient solution treatment using three factors and three level orthogonal tests. To obtain the best ordinary nutrient solution, we tested hydroponic seedling growth treated with the best ordinary nutrient solutions and special nutrient solutions made by the authors using a single factor test.【Result】The orthogonal test of nutrient solution showed that the most suitable ordinary nutrient solution was the 1/4 Hewitt formula (A3B3). In this condition of pH 6.5, the survival rate was 85.71%, the number of leaves was 1.60, the average number of roots was 4.40, the average number of second roots was 25.90, and the fresh root weight was 0.40 g. The soluble sugar content, starch content, soluble proteins content, and the rooting activity were significantly higher than those in the other treatments, although the POD activity was significantly lower than that in the other treatment. The single factor test of the nutrient solutions showed that the most suitable nutrient solution for C. betulus growth was the special nutrient solution No. 1. Under this condition, the survival rate was 93.33%, the soluble sugar content was 0.13 mg/g, the starch content was 2.55 mg/g, the soluble proteins content was 33.33 mg/g, and the rooting activity was 49.75 μg/(g·h).【Conclusion】The most suitable nutrient solution for C. betulus was the special nutrient solution No.1 prepared by us.

Key words: Carpinus betulus, hydroponic culture, nutrient solution, roots growth, soluble sugav content

CLC Number:

S718
S687

HUO Yan, ZHANG Huihui, ZHU Zunling. Comparative study on the effects of nutrient solution treatment in hydroponic cultivation of Carpinus betulus[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 17-24.

Figures/Tables 8

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References 33

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处理 treatment	存活率/% survival rate	叶片数 leaf number	平均根数 average root number	平均二级根数 average second root number	根鲜质量/g fresh root weight
A1B1C1	64.29±0.71 Ed	1.30±0.02 Cc	1.44±0.03 Ee	7.47±0.02 ff	0.37±0.02 ABabc
A1B2C2	64.20±0.55 Ed	1.32±0.02 Cc	2.00±0.05 Cc	6.09±0.04 Gg	0.43±0.04 Aab
A1B3C3	92.86±0.14 Aa	1.59±0.02 Aa	2.31±0.04 Bb	18.83±0.08 Cc	0.36±0.04 ABCbc
A2B1C2	42.86±2.86 Fc	0.80±0.10 Ef	1.33±0.05 Ef	1.99±0.11 Hh	0.25±0.05 Cd
A2B2C3	64.29±1.67 Ed	1.11±0.04 De	1.67±0.07 Dd	6.14±0.04 Gg	0.33±0.02 ABCcd
A2B3C1	78.57±2.57 CDc	1.63±0.02 Aa	2.27±0.06 Bb	14.60±0.04 Dd	0.27±0.07 BCd
A3B1C3	83.33±1.67 BCb	1.20±0.03 Dd	1.97±0.05 Cc	19.81±0.05 Bb	0.44±0.06 Aa
A3B2C1	78.57±3.50 Dc	1.45±0.04 Bb	2.33±0.05 Bb	13.16±0.04 Ee	0.40±0.09 Aabc
A3B3C2	85.71±2.29 Bb	1.60±0.03 Aa	4.40±0.05 Aa	25.90±0.03 Aa	0.40±0.03 Aabc

因子 factor	存活率/% survival rate	叶片数 leaf number	平均根数 average root number	平均二级根数 average second root number	根鲜质量/g fresh root weight
A1	74.20±14.01 Bb	1.40±0.14 Aa	1.92±0.38 Bb	10.80±6.05 Bb	0.39±0.04 Aa
A2	61.59±15.66 Cc	1.18±0.37 Bb	1.76±0.42 Bb	7.58±5.57 Cc	0.28±0.06 Bb
A3	81.85±4.71 Aa	1.42±0.18 Aa	2.90±1.14 Aa	19.62±5.52 Aa	0.41±0.06 Aa
B1	63.49±17.62 Cc	1.10±0.24 Cc	1.58±0.30 Cc	9.76±7.90 Bb	0.35±0.09 Aa
B2	68.43±6.43 Bb	1.30±0.15 Bb	2.00±0.29 Bb	8.46±3.52 Bb	0.39±0.06 Aa
B3	85.71±6.42 Aa	1.61±0.03 Aa	2.99±1.06 Aa	19.78±4.94 Aa	0.34±0.07 Aa
C1	63.49±7.04 Cc	1.46±0.15 Aa	2.02±0.43 Bb	11.74±3.27 Bb	0.35±0.08 Aa
C2	68.43±18.66 Bb	1.24±0.36 Bb	2.58±1.40 Aa	11.33±11.07 Bb	0.36±0.09 Aa
C3	85.71±13.11 Aa	1.30±0.22 Bb	1.98±0.28 Bb	14.93±6.60 Aa	0.38±0.06 Aa

指标 index	存活率 survival rate	叶片数 leaf number	平均根数 average root number	平均二级根数 average second root number
存活率
叶片数	0.836^**
平均根数	0.613^**	0.644^**
平均二级根数	0.892^**	0.705^**	0.811^**
根鲜质量	0.454^*	0.282	0.275	0.359

处理 treatment	可溶性糖含量/ (mg·g^-1) soluble sugar content	淀粉含量/ (mg·g^-1) starch content	可溶性蛋白含量/ (mg·g^-1) soluble proteins content	POD活性/ (U·g^-1) POD activity	根系活力/ (μg·g^-1·h^-1) root activity
A1B1C1	0.12±0.00 Aa	2.30±0.22 BCbc	19.23±0.31 Df	86.66±11.55 Cc	19.23±3.00 Df
A1B2C2	0.11±0.01 ABab	2.03±0.07 BCc	49.00±0.43 Aa	183.33±15.28 b	49.00±3.00 Aa
A1B3C3	0.13±0.02A Bab	2.00±0.08 Cc	38.13±0.09 Bb	40.00±10.00 Cc	38.13±3.00 Bb
A2B1C2	0.08±0.01 Cc	2.51±0.07 Bb	10.55±0.12 Eg	270.00±79.37 Aa	10.55±2.00 Eg
A2B2C3	0.08±0.01 Cc	3.04±0.14 Aa	31.68±0.39 BCcd	220.00±20.00 ABab	31.68±4.04 BCcd
A2B3C1	0.12±0.02 Aa	2.23±0.06 BCbc	35.29±0.19 BCbc	233.33±23.09 ABab	35.29±3.00 BCbc
A3B1C3	0.10±0.02 ABCbc	2.22±0.11 BCbc	31.25±0.93 BCcd	93.33±11.55 Cc	31.25±3.51 BCcd
A3B2C1	0.12±0.01 ABab	2.21±0.08 BCbc	36.88±0.18 Bbc	60.00±20.00 Cc	36.88±3.00 Bbc
A3B3C2	0.08±0.02 BCc	2.34±0.56 BCbc	28.70±1.996 Cd	220.00±26.46 ABab	28.76±3.00 Cd

因子 factor	可溶性糖含量/ (mg·g^-1) soluble sugar content	淀粉含量/ (mg·g^-1) starch content	可溶性蛋白含量/ (mg·g^-1) soluble proteins content	POD活性/ (U·g^-1) POD activity	根系活力/ (μg·g^-1·h^-1) root activity
A1	0.11±0.01 Aa	2.11±0.19 Bb	36.39±0.32 Bb	103.33±64.23 Bb	35.45±13.30 Aa
A2	0.09±0.02 Bb	2.59±0.37 Aa	35.45±0.67 Bc	241.11±48.07 Aa	25.84±11.89 Bb
A3	0.10±0.02 Ab	2.25±0.29 Ab	37.92±1.30 Aa	124.44±75.18 Bb	32.30±4.53 Aa
B1	0.10±0.02 Aa	2.34±0.18 Aa	36.50±1.63 Aa	150.00±98.74 Aa	20.34±9.35 Bb
B2	0.10±0.02 Aa	2.43±0.48 Aa	37.00±1.17 Aa	154.44±74.35 Aa	39.19±8.23 Aa
B3	0.10±0.02 Aa	2.19±0.32 Aa	36.27±1.18 Ab	164.44±95.28 Aa	34.06±4.91 Aa
C1	0.12±0.01 Aa	2.25±0.13 Aa	36.89±1.30 Aa	126.67±82.46 Bb	30.47±8.85 Aa
C2	0.09±0.02 Bb	2.29±0.35 Aa	36.03±1.47 Ab	224.44±56.81 Aa	29.44±16.82 Aa
C3	0.09±0.02 Bb	2.42±0.48 Aa	36.84±1.17 Aa	117.78±81.05 Bb	33.69±4.53 Aa

Comparative study on the effects of nutrient solution treatment in hydroponic cultivation of Carpinus betulus

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指标 index	可溶性糖含量 soluble sugar content	淀粉含量 starch content	可溶性蛋白含量 soluble proteins content	POD活性 POD activity
可溶性糖含量
淀粉含量	-0.544^**
可溶性蛋白含量	0.136	-0.011
POD活性	-0.407^*	0.465^*	-0.516^**
根系活力	0.282	-0.330	0.278	-0.250

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