【Objective】Stem segments having buds of Clematis ‘Julka’ were used as the explants to construct a regeneration system. 【Method】The system was established via two ways: one is initiates from induction of axillary buds, then proliferation of adventitious buds, and rooting induction;the other is from callus induction and proliferation to differentiation, final rooting. 【Result】The method for explants surface sterilization was incubated in 10% (mass fraction) sodium hypochlorite for 12 min. The medium for inducing axillary buds was MS+NAA 0.1 mg/L+6-BA 1.0 mg/L and for adventitious buds proliferation was MS+IBA 0.20 mg/L+6-BA 1.0 mg/L+GA₃ 0.2 mg/L. The medium for callus induction was MS+NAA 0.05 mg/L+6-BA 2.0 mg/L, for callus proliferation was MS+NAA 0.20 mg/L +6-BA 2.0 mg/L, and for callus differentiation was MS+ NAA 0.03 mg/L +6-BA 3.0 mg/L. The Optimal rooting induction medium was 1/2MS+IBA 0.3 mg/L. 【Conclusion】This study established a stable and high-efficient regeneration system in vitro for Clematis ‘Julka’ by using two ways of organogenesis. The multiplication rate was 5.52 when shoot organogenesis were formed directly. In the organogenesis of callus induction pathway, the ratio of callus differentiation was 76.7% and the average number of differentiated adventitious buds was more than 3. The rates of plantlet rooting in two ways were more than 90%.