The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora

PAN Min, ZHU Minglong, TAN Jiajin, LI Liangliang, HAO Dejun

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3) : 151-156.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2022, Vol. 46 ›› Issue (3) : 151-156. DOI: 10.12302/j.issn.1000-2006.202001008

The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora

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Abstract

【Objective】The Bacillus pumilus LYMC-3 strain has a considerable control effect on the rice blast, cucumber fusarium wilt, and cotton verticillium wilt. To further research on the biocontrol potential of this strain, the antibacterial spectrum was tested by measuring the antibacterial activity of LYMC-3 strain against eight types of plant pathogenic fungi to provide a reference for its subsequent popularization and application.【Method】The antibacterial activity of LYMC-3 strain against eight plant pathogenic fungi (Sphaeropsis sapinea, Fusarium circinatum, Cytospora chrysosperma, Phomopsis macrospora, Fusicoccum aesculi, Pestalotiopsis theae, Guignardia camelliae and Botryosphaeria dothiorella) was determined using the plate pairing method, and one of them was selected for the mycelial growth and spore germination inhibition tests.【Result】The LYMC-3 strain had a wide antimicrobial spectrum, with inhibitory effects on many plant pathogenic fungi that were tested. The length of the inhibitory zone and the magnitude of the antibacterial activity were in the following order: Phomopsis macrospora > Pestalotiopsis theae > Botryosphaeria dothiorella > Sphaeropsis sapinea > Fusicoccum aesculi > Cytospora chrysosperma > Fusarium circinatum > Guignardia camelliae. The inhibition rate of the fermentation broth of the LYMC-3 strain to the mycelial growth of Phomopsis macrospora was 70.36%, and the spore germination in 24 hours was 88.16%.【Conclusion】The Bacillus pumilus LYMC-3 strain has a wide antimicrobial spectrum. The fermentation filtrate broth of the strain has a high antagonistic effect on Phomopsis macrospora.

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Bacillus pumilus / antimicrobial spectrum / Phomopsis macrospora / plant pathogenic fungi

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PAN Min , ZHU Minglong , TAN Jiajin , et al . The antagonism of Bacillus pumilus LYMC-3 strain on Phomopsis macrospora[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2022, 46(3): 151-156 https://doi.org/10.12302/j.issn.1000-2006.202001008

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