Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

doi:10.12302/j.issn.1000-2006.202003012

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 120-126.doi: 10.12302/j.issn.1000-2006.202003012

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Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

SHI Xinlong¹^,²(), YANG Yueqin¹, XUE Xian¹^,², LIU Wei¹^,², SONG Chengwei¹^,², GUO Lili¹^,², HOU Xiaogai¹^,²^,^*()

1. College of Agriculture / College of Tree Penoy,Henan University of Science and Technology,Luoyang 471023,China
2. Henan Province Oil Tree Peony Engineering Technology Research Center,Luoyang 471023,China

Received:2020-03-10 Accepted:2020-07-17 Online:2021-03-30 Published:2021-04-09
Contact: HOU Xiaogai E-mail:sxl87@163.com;hkdhxg@haust.edu.cn

Abstract

Abstract:

【Objective】This study was carried out to investigate the effects of chitooligosaccharide(COS) on the growth, physiology and drought resistance mechanisms of tree peony under drought stress, which provides a theoretical basis for the study of environmental selection and drought resistance during seedling cultivation.【Method】To investigate alleviation of drought stress in plants via COS, biennial Paeonia ostii ‘Feng Dan’ seedlings under different drought stress conditions (water withheld for 7, 9, 11, 13, 15 d) were treated with COS at five concentrations (0, 20, 40, 60, 80 mg/L), and the treated seedlings were collected for physiological analysis.【Result】Compared with the control, treatments that provided exogenous COS during drought stress promoted the growth of tree peony seedlings, thereby markedly improving their relative water content and contents of chlorophyll, soluble sugar(SS), and soluble protein(SP), coupled with an enhanced net photosynthetic rate. Moreover, the superoxide dismutase(SOD), peroxidase(POD), and catalase(CAT) activities of P. ostii ‘Feng Dan’ were significantly increased, and the contents of malondialdehyde(MAD) and H₂O₂ were significantly reduced.【Conclusion】COS treatment at 40 mg/L could effectively alleviate drought damage and enhance resistance in P. ostii ‘Feng Dan’ seedlings.

Key words: Paeonia ostii ‘Feng Dan’, drought stress, chitooligosaccharide, biomass, antioxidant enzyme, osmoregulatory substance

CLC Number:

S512.6

SHI Xinlong, YANG Yueqin, XUE Xian, LIU Wei, SONG Chengwei, GUO Lili, HOU Xiaogai. Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 120-126.

Figures/Tables 5

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处理/ (mg·L^-1) treatment	苗高/cm seedling height	茎粗/mm stem diameter	地上生物量/g above-ground biomass	地下生物量/g under-ground biomass	总生物量/g total biomass	根冠比 root-shoot ratio
CK	14.53±1.00 c	4.61±0.27 b	3.72±0.26 b	3.19±0.22 b	6.91±0.48 b	0.86±0.01 a
20	15.33±0.47 bc	5.11±0.23 a	4.29±0.08 b	3.23±0.05 b	7.52±0.03 b	0.75±0.02 b
40	18.23±0.95 a	5.43±0.19 a	5.43±0.26 a	3.85±0.37 a	9.28±0.43 a	0.71±0.08 b
60	16.67±0.81 b	5.18±0.12 a	5.57±0.41 a	3.72±0.28 ab	9.29±0.66 a	0.66±0.03 b
80	16.00±0.76 bc	5.11±0.16 a	5.25±0.54 a	3.45±0.14 ab	8.71±0.49 a	0.66±0.08 b

Effects of chitooligosaccharide on the growth physiology of Paeonia ostii ‘Feng Dan’ seedlings under drought stress

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