Effects of two plant growth regulators on rooting of Hibiscus syriacus cuttings

doi:10.12302/j.issn.1000-2006.202003018

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (3): 123-129.doi: 10.12302/j.issn.1000-2006.202003018

Previous Articles Next Articles

Effects of two plant growth regulators on rooting of Hibiscus syriacus cuttings

ZHANG Lin(), CHENG Ya’nan, ZHANG Xin, YANG Weiting, KONG Qingtao, XIE Dongfeng^*()

College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China

Received:2020-03-04 Revised:2020-07-02 Online:2021-05-30 Published:2021-05-31
Contact: XIE Dongfeng E-mail:lin145698218@163.com;xiedongfeng@lyu.edu.cn

Abstract

Abstract:

【Objective】The aim of this study was to understand the rooting mechanism of Hibiscus syriacus cuttings, including the relevant effects of two plant growth regulators and their suitable formula, as well as to improve the rooting rate and provide a theoretical support for the propagation of H. syriacus cuttings. 【Method】One-year-old branches of H. syriacus seedlings were collected as experimental materials. A completely randomized block design with two factors, including two plant growth regulators (naphthylacetic acid, NAA; indolebutyric acid, IBA) at three concentrations (100, 300 and 600 mg/L), were used. One water-treated sample was used as a control (CK). The anatomical structure, rooting growth characteristics, and leaf physiological characteristics of H. syriacus cuttings were measured during the rooting process using a UY203i biological fluorescence microscope, a STD4800 scanner calibrated for an image analysis with WinRHIZO software, a JA5003N one-thousandth electronic balance, a STYS-1 plant chlorophyll measuring instrument in vivo, and a FS-3080H photosynthesis system. The measured indexes included the rooting rate (RR), root fresh weight (RFW), root TTC reducing ability (RA), root TTC reducing amount (TTC_RA), average root length (ARL), root average diameter (RAD), root surface area (SA), root volume (RV), root tip number(RT), root length density (RLD), specific root length (SRL), chlorophyll content (Chl) and net photosynthetic rate (P_n) in the rooting process. 【Result】① It was found by microscopic observation that the adventitious root primordium of H. syriacus cuttings originated from a group of parenchyma cells with a blunt conical shape located in the cross region of pith rays and vascular cambium. The adventitious root primordia developed into young adventitious roots and then extended outward through the lenticels. ② Compared with the control (CK), the rooting rate (RR), root fresh weight (RFW), root TTC reducing ability (RA), root TTC reducing amount (TTC_RA), average root length (ARL), root average diameter (RAD), root surface area (SA), root volume (RV), root tip number (RT), and root length density (RLD) of 300 mg/L IBA treatment increased by 554%, 527%, 58%, 892%, 198%, 50%, 282%, 457%, 126% and 198%, respectively, and its specific root length (SRL), chlorophyll content (Chl), and net photosynthetic rate (P_n) were reduced by 52%, 51% and 82%, respectively. ③ According to the correlation analysis, the Chl and P_n of H. syriacus seedlings had a negative relationship with most of the root indicators. Chl was significantly negatively correlated with SA and very significantly negatively correlated with ARL and RLD. P_n was very significantly negatively correlated with RA and significantly negatively correlated with ARL and RLD. ④ Using the subordinate function value method that the rooting ability of the IBA treatment exceeded that of the NAA treatment, and the rooting ability of the 300 mg/L IBA treatment was the best. 【Conclusion】 The cortex rooting type was the main type of rooting found in H. syriacus cuttings. There was a competitive relationship between the overground parts and the underground parts of H. syriacus cuttings during the rooting process. Two plant growth regulators could promote rooting of H. syriacus cuttings and inhibit the physiological characteristics of its overground parts. The treatment with 300 mg/L IBA provided the most suitable formula.

Key words: Hibiscus syriacus, plant growth regulator, cutting, rooting rate

CLC Number:

S723.1

ZHANG Lin, CHENG Ya’nan, ZHANG Xin, YANG Weiting, KONG Qingtao, XIE Dongfeng. Effects of two plant growth regulators on rooting of Hibiscus syriacus cuttings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 123-129.

Figures/Tables 6

Fig.1

Fig.2

Fig.3

Fig.4

Table 1

Table 2

References 20

[1]	孙居文, 王秀林, 田知理, 等. 园林树木学[M]. 上海: 上海交通大学出版社, 2008: 187.
	SUN J W, WANG X L, TIAN Z L, et al. Landscape dendrology[M]. Shanghai: Shanghai Jiao Tong University Press, 2008: 187.
[2]	兰菊梅, 徐秀琴, 余京文. 六盘山林区木槿的硬枝扦插繁育技术[J]. 青海农林科技, 2014(4):85-86.
	LAN J M, XU X Q, YU J W. Hardwood cuttings technology of Hibiscus syriacus Liupan Mountain[J]. Sci Technol Qinghai Agric For, 2014(4):85-86.
[3]	李芳. 木槿扦插繁殖技术[J]. 农业工程技术, 2016,36(17):67.
	LI F. Cutting technology of Hibiscus syriacus[J]. Agric Eng Technol, 2016,36(17):67. DOI: 10.16815/j.cnki.11-5436/s.2016.17.054.
[4]	di MARTINO O, TITO A, de LUCIA A, et al. Hibiscus syriacus extract from an established cell culture stimulates skin wound healing[J]. Biomed Res Int, 2017: 7932019. DOI: 10.1155/2017/7932019.
[5]	YOON S W, LEE K P, KIM D, et al. Effect of absolute from Hibiscus syriacus L. flower on wound healing in keratinocytes[J]. Pharmacogn Mag, 2017,13(49):85-89. DOI: 10.4103/0973-1296.197644.
[6]	KIM T, PARK J H, LEE S G, et al. Small RNA transcriptome of Hibiscus syriacus provides insights into the potential influence of microRNAs in flower development and terpene synjournal[J]. Mol Cells, 2017,40(8):587-597. DOI: 10.14348/molcells.2017.0086. doi: 10.14348/molcells.2017.0086
[7]	芦治国, 殷云龙, 於朝广, 等. NaCl胁迫条件下木槿嫁接苗和扦插苗及其砧木海滨木槿的生理差异[J]. 植物资源与环境学报, 2011,20(4):49-57.
	LU Z G, YIN Y L, YU C G, et al. Physiological difference among grafted and cutting seedlings of Hibiscus syriacus and its rootstock H. hamabo under NaCl stress[J]. J Plant Resour Environ, 2011,20(4):49-57. DOI: 10.3969/j.issn.1674-7895.2011.04.009.
[8]	LI P, CALATAYUD V, GAO F, et al. Differences in ozone sensitivity among woody species are related to leaf morphology and antioxidant levels[J]. Tree Physiol, 2016,36(9):1105-1116. DOI: 10.1093/treephys/tpw042. doi: 10.1093/treephys/tpw042
[9]	王晓红, 任雪羽, 刘佳, 等. 影响木槿扦插生根的因素分析[J]. 中南林业科技大学学报, 2018,38(4):6-9,15.
	WANG X H, REN X Y, LIU J, et al. Analysis on factors of affecting rooting of Hibiscus syriacus[J]. J Central South Univ For Technol, 2018,38(4):6-9,15. DOI: 10.14067/j.cnki.1673-923x.2018.04.002.
[10]	GRANGE R I, LOACH K. The effect of light on the rooting of leafy cuttings[J]. Sci Hortic, 1985,27(1/2):105-111. DOI: 10.1016/0304-4238(85)90060-3. doi: 10.1016/0304-4238(85)90060-3
[11]	王学奎. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2006.
	WANG X K. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2006.
[12]	王竞红, 陈鹏, 陈艾, 等. 3种观赏草苗期对干旱胁迫的响应及抗旱性评价[J]. 草业科学, 2019,36(5):1266-1274.
	WANG J H, CHEN P, CHEN A, et al. Effects of drought stress on physiological and growth characteristics of the seedlings of three ornamental grasses[J]. Pratacultural Sci, 2019,36(5):1266-1274. DOI: 10.11829/j.issn.1001-0629.2018-0388.
[13]	李焕勇, 刘涛, 张华新, 等. 植物扦插生根机理研究进展[J]. 世界林业研究, 2014,27(1):23-28.
	LI H Y, LIU T, ZHANG H X, et al. Research progress in rooting mechanism of plant cuttings[J]. World For Res, 2014,27(1):23-28. DOI: 10.13348/j.cnki.sjlyyj.2014.01.005.
[14]	郭素娟. 林木扦插生根的解剖学及生理学研究进展[J]. 北京林业大学学报, 1997,19(4):64-69.
	GUO S J. Progress of study on rooting anatomy and physiology of forest tree cuttings[J]. J Beijing For Univ, 1997,19(4):64-69. DOI: 10.13332/j.1000-1522.1997.04.011.
[15]	张丹丹, 范俊俊, 赵明明, 等. 外施植物生长调节剂对细柄阿丁枫扦插生根的影响[J]. 南京林业大学学报(自然科学版), 2018,42(1):41-47.
	ZHANG D D, FAN J J, ZHAO M M, et al. Effects of plant growth regulator on cuttings rooting of Altingia gracilipessl[J]. J Nanjing For Univ (Nat Sci Ed), 2018,42(1):41-47. DOI: 10.3969/j.issn.1000-2006.201609057.
[16]	秦爱丽, 简尊吉, 马凡强, 等. 母树年龄、生长调节剂、容器与基质对崖柏嫩枝扦插的影响[J]. 林业科学, 2018,54(7):40-50.
	QIN A L, JIAN Z J, MA F Q, et al. Effects of the mother tree age,growth regulator,containers and substrates on softwood cutting propagation of Thuja sutchuenensis[J]. Sci Silvae Sin, 2018,54(7):40-50. DOI: 10.11707/j.1001-7488.20180705.
[17]	王艺, 贾忠奎, 马履一, 等. 4种植物生长调节剂对红花玉兰嫩枝扦插生根的影响[J]. 林业科学, 2019,55(7):35-45.
	WANG Y, JIA Z K, MA L Y, et al. Effects of four plant growth regulators on rooting of the softwood cutting of Magnolia wufengensis[J]. Sci Silvae Sin, 2019,55(7):35-45. DOI: 10.11707/j.1001-7488.20190704.
[18]	魏黔春, 江泽平, 刘建锋, 等. 侧柏古树扦插试验及插穗营养物质变化[J]. 南京林业大学学报(自然科学版), 2020,44(1):63-71.
	WEI Q C, JIANG Z P, LIU J F, et al. Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material[J]. J Nanjing For Univ (Nat Sci Ed), 2020,44(1):63-71. DOI: 10.3969/j.issn.1000-2006.201903057.
[19]	尚秀华, 高丽琼, 谢耀坚, 等. 油料植物南美油藤扦插技术研究[J]. 中南林业科技大学学报, 2017,37(2):14-18.
	SHANG X H, GAO L Q, XIE Y J, et al. Studies on cutting technique of oil plant Plukenetia volubilis[J]. J Central South Univ For Technol, 2017,37(2):14-18. DOI: 10.14067/j.cnki.1673-923x.2017.02.003.
[20]	TWORKOSKI T, TAKEDA F. Rooting response of shoot cuttings from three peach growth habits[J]. Sci Hortic, 2007,115(1):98-100. DOI: 10.1016/j.scienta.2007.08.004. doi: 10.1016/j.scienta.2007.08.004

Related Articles 15

[1]	BU Xiaoting, FU Wei, LI Shuxian, XU Zhibiao, PENG Daqing, XU Linqiao. Effects of rejuvenation and hormone treatment on the rooting of softwood cuttings of Quercus texana and anatomical observations of rooting [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2024, 48(2): 129-136.
[2]	WANG Yu, YI Yanling, LIU Hai, WEN Xiaochen, LI Tianyi, YIN Haifeng, LI Xianwei, FAN Chuan. Initial impacts of two thinning methods on the spatial structure of Pinus massoniana plantations [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(5): 138-146.
[3]	XIAO Xiao, ZHOU Yang, WANG Shumei, ZHENG Yaxiong, GUAN Fengying. Effects of strip cutting on spatial structure and stability of new bamboo (Phyllostachys edulis) [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2023, 47(4): 139-147.
[4]	CHEN Han, WANG Dongsheng, BAI Bing, LI Jiafeng, CHEN Kexin, CHENG Beibei. Phenotypic diversity of 21 Hibiscus cultivars [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2022, 46(3): 117-126.
[5]	WANG Shumei, FAN Shaohui, XIAO Xiao, ZHENG Yaxiong, ZHOU Yang, GUAN Fengying. Effects of strip cutting on aboveground biomass accumulation and allocation, and allometric growth of Phyllostachys edulis [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 19-24.
[6]	WEI Qingyu, HUANG Hailong, WU Chunze, SU Jiaxi, WEI Xing. Response of Populus cathayana cutting seedlings of three ploidy types to fertilizer under film mulching and drip irrigation [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(5): 93-101.
[7]	WU Lijun, YOU Yunfei, CHEN Da, CHEN Wenrong, LI Wenfang. Optimization of the rooting and transplantation techinques of tissue-cultured shoots of Camellia chrysanthoides ‘Huangzun’ [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(3): 117-122.
[8]	WANG Shumei, WANG Bo, FAN Shaohui, XIAO Xiao, XIA Wen, GUAN Fengying. Influence of strip cutting management on soil bacterial community structure and diversity in Phyllostachys edulis stands [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 60-68.
[9]	WANG Gaiping, WANG Lianggui, WANG Xiaocong, ZHANG Chen, ZHANG Lei, LIU Bin. Dynamic characteristics of cutting rooting of Catalpa bungei with tender branches [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(6): 94-102.
[10]	JING Ruyue, WANG Peilan, HUANG Zhen, HE Linjun, LI Zhihui. Effects of different carbon sources on somatic embryo of Cinnamomum camphora L. [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(1): 54-62.
[11]	WEI Qianchun, JIANG Zeping, LIU Jianfeng, SHI Shengqing, ZHAO Xiulian, CHANG Ermei. Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2020, 44(1): 63-71.
[12]	ZHAO Xiang, LI Qingying, JIANG Qingbin, ZHONG Chonglu, ZHANG Jie . Effects of substrates and rooting regulators on rooting of cuttings in Manglietia conifera Dandy [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2019, 43(02): 23-30.
[13]	WU Lijun, GAO Nan, CHEN Da, CHEN Wenrong. Tissue culture and rapid propagation of sterile seedling of Camellia nitidissima C. W. Chi hybrid germplasm [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(05): 32-38.
[14]	WEI Dongshan, ZHANG Qiuliang, CHANG Jinbao. A study on mechanism of propagation and shoot cutting of Monarda didyma [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(05): 39-45.
[15]	DENG Xiaomei, WU Qiaona, LI Ruiping, ZHAO Mengqiu, LIN Jieying, XI Ruchun. Research progress on in vitro micropropagation of Fagaceae plants [J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2018, 42(04): 171-180.

Metrics

Comments

www.nldxb.njfu.edu.cn

Edited ＆ Published by Editorial Department of Nanjing Forestry University(Natural Sciences Edition)
Address： No.159 Longpan Road,Nanjing 210037 Jiangsu,P.R.China
E-mail ：xuebao@njfu.edu.cn , xuebao@njfu.com.cn
Telephone +86-25-85428247,85427076
Distributed by China International Book Trading Corporation P.O. Box 399, Beijing ,P.R. China

指标 index	RR	RA	TTC_RA	ARL	RAD	SA	RV	RT	RLD	RFW	SRL	Chl	P_n
RR	1
RA	0.139	1
TTC_RA	0.666	0.764^*	1
ARL	0.203	0.858^*	0.742	1
RAD	0.785^*	-0.389	0.218	-0.140	1
SA	0.630	0.673	0.920^**	0.851^*	0.335	1
RV	0.821^*	0.383	0.859^*	0.578	0.640	0.919^**	1
RT	0.183	0.791^*	0.529	0.684	-0.273	0.495	0.230	1
RLD	0.203	0.858^*	0.742	1.000^**	-0.140	0.851^*	0.578	0.684	1
RFW	0.794^*	0.630	0.975^**	0.633	0.395	0.903^**	0.913^**	0.488	0.633	1
SRL	-0.807*	-0.024	-0.480	0.165	-0.602	-0.273	-0.516	-0.130	0.165	-0.625	1
Chl	-0.183	-0.653	-0.625	-0.879^**	0.118	-0.758^*	-0.538	-0.384	-0.879^**	-0.514	-0.306	1
P_n	-0.352	-0.880^**	-0.753	-0.776^*	0.157	-0.676	-0.458	-0.602	-0.776^*	-0.634	0.099	0.672	1

处理 treatment	隶属函数值 membership function value													平均值 mean	排序 sort
处理 treatment	RR	RA	TTC_RA	ARL	RAD	SA	RV	RT	RLD	RFW	SRL	Chl	P_n	平均值 mean	排序 sort
CK	0	0	0	0	0	0.30	0	0	0	0	0.19	0.17	0	0.05	7
T1	0.51	0.18	0.13	0.11	0.47	0.74	0.36	0.30	0.42	0.47	0.13	0.79	0.45	0.39	5
T2	0.65	0.42	0.38	0.48	0.15	0.46	0.18	0.16	0.69	0.15	1.00	0.10	0.72	0.43	4
T3	0.44	0.10	0.06	0.02	0.09	0.88	0.16	0.20	0.09	0.09	0.49	0	0.47	0.24	6
T4	0.09	0.35	0.46	1.00	1.00	0	0.53	0.21	1.00	1.00	0	0.83	0.98	0.57	2
T5	1.00	1.00	1.00	0.74	0.88	1.00	1.00	1.00	0.52	0.88	0.91	1.00	1.00	0.92	1
T6	0.65	0.46	0.32	0.15	0.30	1.00	0.43	0.47	0.67	0.30	0.89	0.02	0.12	0.45	3

Effects of two plant growth regulators on rooting of Hibiscus syriacus cuttings

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 20

Related Articles 15

Recommended Articles

Metrics

Comments

Author

Reader

Reviewer