Effects of AMF inoculation and nitrogen application on nitrogen mineralization of coastal saline soil

doi:10.12302/j.issn.1000-2006.202003087

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (2): 145-153.doi: 10.12302/j.issn.1000-2006.202003087

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Effects of AMF inoculation and nitrogen application on nitrogen mineralization of coastal saline soil

YANG Ruizhen(), ZHANG Huanchao^*(), HU Lihuang, FAN Zhixin

Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Received:2020-03-31 Accepted:2020-04-20 Online:2021-03-30 Published:2021-04-09
Contact: ZHANG Huanchao E-mail:yrzz01@163.com;hczhang@njfu.edu.cn

Abstract

Abstract:

【Objective】This research aimed to to study the effects of arbuscular mycorrhizal fungi (AMF) inoculation and nitrogen application on the nitrogen mineralization and explore a mechanism to improve the nitrogen supply capacity of coastal saline soils. 【Method】AMF and different nitrogen concentrations were applied to Jiangsu coastal saline soils to evaluate nitrogen mineralization by using laboratory incubation methods. 【Result】Nitrogen had a significant effect on the mycorrhizal infection rate (P <0.05). The mycorrhizal of the low nitrogen treatment (N1, 92 mg/kg) reached a 45.22% infection rate, which was significantly higher than that of the high nitrogen (N2, 184 mg/kg) and control treatments, with 32.44% and 16.00%, respectively. Under the AMF treatment, the content of ammonium nitrogen in the low nitrogen treatment was significantly lower than that in the high nitrogen and control treatments (P <0.05). The higher mycorrhizal infection rate was more conducive to plant absorption and assimilation of ammonium nitrogen. Nitrogen application and inoculation treatment significantly affected the ammoniation process of coastal saline soils (P < 0.05) but did not interact with it. The content of nitrate nitrogen increased with an increase in the nitrogen application. The nitrate nitrogen content in the high nitrogen treatment was significantly higher than that of the low nitrogen and control treatments (P < 0.05). The amount of mineralized nitrogen and the net mineralization rate were not significantly different after the nitrogen application and AMF inoculation (P > 0.05); however, the combined treatment had an interaction effect, which significantly promoted mineralization (P < 0.001). 【Conclusion】For coastal saline soils, excessive nitrogen addition inhibits the establishment of a symbiotic relationship between AMF and salt-tolerant plants; nitrogen application was beneficial to nitrogen mineralization; however, excessive nitrogen did not improve nitrogen supply capacity; the establishment of a symbiotic relationship between AMF and plants could effectively improve the efficiency of nitrogen fertilizer utilization. Nitrogen application and AMF inoculation had a certain interaction effect on the process of nitrogen mineralization in coastal saline soil.

Key words: nitrogen mineralization, nitrogen application, arbuscular mycorrhizal fungi (AMF), coastal saline soil, $\text{NO}_{3}^{-}-\text{N}$, $\text{NH}_{4}^{+}-\text{N}$, soil improvement

CLC Number:

YANG Ruizhen, ZHANG Huanchao, HU Lihuang, FAN Zhixin. Effects of AMF inoculation and nitrogen application on nitrogen mineralization of coastal saline soil[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY, 2021, 45(2): 145-153.

Figures/Tables 7

Table 1

Fig.1

Fig.2

Table 2

The content of nitrate nitrogen and ammonium nitrogen in saline soil inoculated with AMF and nitrogen treatments at different cultivation timemg/kg"

指标 index	处理 treatments	1 d	3 d	5 d	7 d	10 d	20 d	30 d
硝态氮 $NO 3 -$ -N	N0-	51.78±0.08 e	67.06±0.64 d	72.83±3.86 c	71.25±2.64 d	72.70±1.57 c	68.03±1.88 d	68.32±0.78 d
	N1-	66.31±1.92 d	84.12±4.27 c	93.45±4.88 b	83.41±4.99 c	92.54±1.42 b	88.62±2.97 c	87.37±1.04 c
	N2-	80.87±0.74 c	94.52±0.25 b	98.57±2.08 ab	88.94±5.69 bc	100.09±3.89 a	94.57±0.63 b	93.83±0.89 b
	N0+	44.21±1.36 f	58.04±0.52 e	67.56±1.70 c	59.10±2.49 e	55.39±2.04 d	62.36±2.81 e	62.05±0.70 e
	N1+	85.05±0.71 b	94.85±2.19 b	99.19±1.02 ab	94.92±0.31 b	98.73±0.85 a	92.27±0.59 bc	92.34±0.46 b
	N2+	86.72±2.90 a	103.60±1.28 a	101.88±1.69 a	106.87±1.88 a	100.13±2.86 a	103.85±1.16 a	103.38±0.25 a
铵态氮 N $H 4 +$ -N	N0-	4.37±0.85 a	5.19±0.67 a	5.84±0.80 a	5.64±1.21 a	3.56±1.03 a	1.86±0.16 a	0.12±0.04 c
	N1-	2.69±0.20 bc	3.12±0.58 c	3.63±0.49 c	3.19±0.17 b	4.17±0.17 a	1.74±0.62 a	0.34±0.20 c
	N2-	3.55±0.25 ab	4.30±0.20 ab	4.51±0.75 bc	5.28±0.63 a	4.51±0.80 a	1.58±0.16 ab	0.34±0.08 c
	N0+	2.85±0.17 bc	4.84±0.19 ab	4.89±0.13 b	4.85±0.17 a	4.72±0.17 a	1.02±0.09 b	0.87±0.04 b
	N1+	1.33±0.17 d	4.06±0.24 b	5.09±0.34 b	5.47±0.21 a	4.61±0.08 a	1.58±0.12 ab	0.93±0.12 ab
	N2+	2.61±0.35 c	4.65±0.34 ab	6.52±0.25 a	5.23±0.52 a	3.49±0.60 a	1.30±0.19 ab	1.18±0.15 a

Table 2

Table 3

Fig.3

Fig.4

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处理 treatments	全氮含量/ (g·kg^-1) total N content	有机质含量/ (g·kg^-1) soil organic matter content	含水率/% water content	电导率/ (μS·cm^-1) conductivity	pH
N0-	0.78±0.03	11.65±0.45	12.58±0.88	293.33±2.05	8.68±0.01
N1-	0.81±0.02	11.55±0.09	12.89±0.71	299.00±0.82	8.48±0.01
N2-	0.80±0.01	11.33±0.40	12.70±0.95	299.33±1.25	8.48±0.01
N0+	0.82±0.05	9.82±0.51	12.50±0.41	412.33±2.05	8.64±0.01
N1+	0.78±0.02	12.40±2.61	12.44±0.70	422.33±2.05	8.56±0.01
N2+	0.81±0.01	10.00±0.45	11.77±0.23	394.33±1.70	8.50±0.01

指标 index	施氮 N treatments		接种 inoculation		交互作用 interaction
指标 index	F	P	F	P	F	P
净硝化量 net nitrification	3.154	NS	9.473	0.010	31.926	<0.001
净氨化量 net ammoniation	17.239	<0.001	68.153	<0.001	0.353	NS
净氮矿化量 net nitrogen mineralization	0.755	NS	0.933	NS	26.995	<0.001
净硝化速率 net nitrification rate	3.134	NS	9.455	0.010	31.857	<0.001
净氨化速率 net ammoniation rate	17.437	<0.001	68.583	<0.001	0.357	NS
净氮矿化速率 net nitrogen mineralization rate	0.760	NS	0.946	NS	26.982	<0.001

Effects of AMF inoculation and nitrogen application on nitrogen mineralization of coastal saline soil

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