Association of SNP loci and candidate genes for growth and wood density in Eucalyptus urophylla × E. tereticornis

ZHU Xianliang, ZHOU Changpin, JIA Cuirong, WENG Qijie, LI Fagen

JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (4) : 143-150.

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南京林业大学学报（自然科学版）
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JOURNAL OF NANJING FORESTRY UNIVERSITY ›› 2021, Vol. 45 ›› Issue (4) : 143-150. DOI: 10.12302/j.issn.1000-2006.202004003

Association of SNP loci and candidate genes for growth and wood density in Eucalyptus urophylla × E. tereticornis

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Abstract

【Objective】Growth and basic wood density are important economic traits of Eucalyptus; thus, searching for candidate genes will facilitate the genetic improvement in this genus.【Method】The interspecific full-sib family of E. urophylla and E. tereticornis was employed in this study. In addition, the correlation between the growth traits (height, dia-meter) and basic wood density of eight-year-old trees was investigated. The extreme phenotypes for each trait were screened for genotyping by sequencing (GBS) and developing SNP loci. Subsequently, association analyses of phenotypes and SNP loci were performed. Finally, SNP loci associated with the growth traits and basic wood density of E. urophylla × E. tereticornis were identified; candidate genes for these related traits were also identified. 【Result】① The phenotypic coefficient of variation ranged from 7.51% to 26.19% among the values of height, diameter, and basic wood density, while a significant positive correlation between the growth traits and basic wood density was found. ② A total of 15 185 whole-genome SNP loci were developed, and a total of 111 SNPs were identified as being significantly associated with growth and basic wood density; among which, strong association signals for diameter were detected on chromosome No.2. ③ In addition, 40 candidate genes related to the growth and basic wood density were identified, which were enriched in 52 GO terms. Gene annotation indicated that the candidate genes were mainly involved in plant stress resis-tance, biotic and abiotic stress, and transcription factor families.【Conclusion】In this study, a new set of SNP loci rela-ted to the growth traits and wood properties of E. urophylla × E. tereticornis were obtained, and dozens of candidate genes were identified. Our results suggest that genes related to stress resistance may play an important role in tree growth and wood formation. This information provides valuable genetic resources for research on Eucalyptus breeding.

Key words

Eucalyptus urophylla×E. tereticornis / genotyping by sequencing / single nucleotide polymorphism(SNP) / phenotypic character / association analysis / candidate genes

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ZHU Xianliang , ZHOU Changpin , JIA Cuirong , et al . Association of SNP loci and candidate genes for growth and wood density in Eucalyptus urophylla × E. tereticornis[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2021, 45(4): 143-150 https://doi.org/10.12302/j.issn.1000-2006.202004003

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